LOCUS       sequence-383923-        6023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LC-Myosin labelled with near-infrared fluorescent protein 
            miRFP718nano.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Oliinyk OS, Ma C, Pletnev S, Baloban M, Toboada C, Sheng H, Yao J, 
            Verkhusha VV
  TITLE     Deep-tissue SWIR imaging using rationally designed small red-shifted
            near-infrared fluorescent protein.
  JOURNAL   Nat Methods. 2022 Dec 1. doi: 10.1038/s41592-022-01683-0.
  PUBMED    36456785
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 Dec 1. doi: 10.1038/s41592-022-01683-0."
COMMENT     Sequence Label: pMyosin-miRFP718nano
FEATURES             Location/Qualifiers
     source          1..6023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(90..110)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        95..424
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      275..410
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     337..356
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        472..519
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             538..912
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1042..1163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1079..1098)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1133..1152
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1212..1228)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1212..1228)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1225..1247)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1236..1252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1260..1266,1267..1284,1285..1290))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1305..1326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1443..1460)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1614..2202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1694..1713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2373..3164,3165..3233))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2996..3015
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3234..3338)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3413..3432)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3604..3983
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3984..4187
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4138..4158
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4232..4251
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4232..4250
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5315..5344
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5360..5377
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5400..5417)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5406..5630
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(5676..6023,1..81)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5763..5782)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5973..5994
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
       61 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
      121 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
      181 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      241 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      301 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
      361 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      421 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
      481 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
      541 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
      601 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
      661 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
      721 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
      781 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
      841 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
      901 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
      961 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     1021 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     1081 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1141 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     1201 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     1261 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     1321 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1381 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     1441 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1501 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1561 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1621 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1681 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1741 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1801 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1861 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1921 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1981 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2041 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2101 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2161 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2221 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     2281 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     2341 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2401 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2461 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2521 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2581 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2641 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2701 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2761 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2821 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2881 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2941 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3001 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     3061 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     3121 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     3181 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     3241 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     3301 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3361 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     3421 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     3481 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     3541 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     3601 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     3661 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3721 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3781 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3841 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3901 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3961 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     4021 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4081 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4141 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
     4201 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
     4261 ctggctagtt aagcttggta ccatggcaaa cctggacaag atgctgaaca ccatcgtgac
     4321 cgaggtgcgc cagttcctgc aagtggacag actctgcgtg ttcaagttcg aggaagatta
     4381 ctccggcaac atcatctacg aagccgtgga cgacggatgg ttgagcatcc tgaagaccca
     4441 cgtgcgggac tgctacttca tggaaaccag aggcgaggaa tacctgcacg gcagatacca
     4501 ggccatcgcc gacatccacc aggccaatct ggcggagagc tatagagact tcctgaccca
     4561 gtaccaggtg cgggccattg tggctgtccc catcctgaag ggcaagaagc tgtggggctt
     4621 gttcagcgcc caccaactgg ccgcccctcg gagctggcag gcctgggaaa tcgagttcct
     4681 gaaacagcaa gccgtggtga tgggcatcgc catccagcag agcggaggtg gtggatcagg
     4741 aggaggtggt tctggaggtg gtggatcagg aggaagatct atgtcgagca agagagccaa
     4801 ggccaagacc accaagaaga ggccccagag ggctacgtcc aatgtcttcg ccatgtttga
     4861 ccagtcccag atccaggagt ttaaggaggc cttcaacatg attgatcaga accgcgatgg
     4921 cttcattgat aaggaggacc tgcacgacat gttggcctct ctggggaaga accccacaga
     4981 cgagtacctg gagggcatga tgaacgaggc gccagggccc atcaacttca ccatgttcct
     5041 cacaatgttt ggggagaagc tgaacggcac agaccccgag gatgtgatcc gcaatgcctt
     5101 tgcctgcttt gatgaggagg cctcaggctt catccacgag gaccacctga gggagctgct
     5161 caccaccatg ggcgaccgat tcacggatga ggaggtggac gagatgtacc gcgaggcacc
     5221 cattgataag aagggcaact tcaactacgt ggagttcact cgcatcctca aacacggcgc
     5281 caaggacaag gacgactaat ctagagggcc cttcgaacaa aaactcatct cagaagagga
     5341 tctgaatatg cataccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
     5401 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     5461 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     5521 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5581 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     5641 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     5701 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5761 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5821 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5881 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5941 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6001 actcaaccct atctcggtct att
//
