LOCUS       sequence-383925-        4457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monomeric near-infrared fluorescent protein miRFP718nano in 
            bacterial plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Oliinyk OS, Ma C, Pletnev S, Baloban M, Toboada C, Sheng H, Yao J, 
            Verkhusha VV
  TITLE     Deep-tissue SWIR imaging using rationally designed small red-shifted
            near-infrared fluorescent protein.
  JOURNAL   Nat Methods. 2022 Dec 1. doi: 10.1038/s41592-022-01683-0.
  PUBMED    36456785
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 Dec 1. doi: 10.1038/s41592-022-01683-0."
COMMENT     Sequence Label: pBAD/His-miRFP718nano
FEATURES             Location/Qualifiers
     source          1..4457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        75..359
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     282..301
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             402..419
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(932..949)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1102..1188
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1280..1307
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1326..1417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1418..1486,1487..2278)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1636..1655)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2449..3037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2938..2957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3191..3208
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3223..3363
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3289..3308)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3449..3471
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(3627..4457,1..48))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
       61 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt
      121 cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta
      181 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag
      241 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
      301 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata
      361 cccgtttttt gggctaacag gaggaattaa ccatggggag ccaccatcac catcaccatg
      421 gcagatctgc agctggtacc atggcaaacc tggacaagat gctgaacacc atcgtgaccg
      481 aggtgcgcca gttcctgcaa gtggacagac tctgcgtgtt caagttcgag gaagattact
      541 ccggcaacat catctacgaa gccgtggacg acggatggtt gagcatcctg aagacccacg
      601 tgcgggactg ctacttcatg gaaaccagag gcgaggaata cctgcacggc agataccagg
      661 ccatcgccga catccaccag gccaatctgg cggagagcta tagagacttc ctgacccagt
      721 accaggtgcg ggccattgtg gctgtcccca tcctgaaggg caagaagctg tggggcttgt
      781 tcagcgccca ccaactggcc gcccctcgga gctggcaggc ctgggaaatc gagttcctga
      841 aacagcaagc cgtggtgatg ggcatcgcca tccagcagag ctagctgaat tcgaagcttg
      901 gctgttttgg tggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa
      961 gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca
     1021 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga
     1081 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1141 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     1201 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     1261 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
     1321 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1381 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1441 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1501 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     1561 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1621 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     1681 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1741 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1801 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1861 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1921 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1981 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2041 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2101 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2161 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2221 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2281 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2341 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2401 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2461 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2521 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2581 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2641 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2701 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2761 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2821 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2881 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2941 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3001 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3061 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3121 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3181 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     3241 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     3301 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     3361 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     3421 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     3481 tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa gcggcatgca
     3541 taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag
     3601 ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc attcactttt
     3661 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
     3721 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
     3781 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
     3841 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc
     3901 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa
     3961 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc
     4021 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct
     4081 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc
     4141 gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag
     4201 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg
     4261 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct
     4321 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc
     4381 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa
     4441 cccgccacca gatgggc
//
