LOCUS       sequence-383932-       13240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Akt1 with a myristoylation signal in avian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK
  TITLE     The akt kinase: molecular determinants of oncogenicity.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5.
  PUBMED    9843996
REFERENCE   2  (bases 1 to 13240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1998-12-8"; volume: "95(25)"; pages: 
            "14950-5"
COMMENT     Sequence Label: RCAS.Sfi myr-Akt1
FEATURES             Location/Qualifiers
     source          1..13240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        502..763
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             7819..7839
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             9292..9318
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        9511..9772
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(10362..10384)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10373..10389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10397..10403,10404..10421,10422..10427))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(10470..10488)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10556..10660
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10661..10729,10730..11521)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10879..10898)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11692..12280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12181..12200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12434..12451
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12466..12606
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12532..12551)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12692..12714
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(12708..12899)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
       61 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
      121 cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg
      181 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
      241 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca
      301 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
      361 ggcggcgatg cgatgtacgg gccagatata cgcgtatctg aggggactag ggtgtgttta
      421 ggcgaaaagc ggggcttcgg ttgtacgcgg ttaggagtcc cctcaggata tagtagtttc
      481 gcttttgcat agggaggggg aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat
      541 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
      601 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
      661 gattggacga accactgaat tccgcattgc agagatattg tatttaagtg cctagctcga
      721 tacaataaac gccatttgac cattcaccac attggtgtgc acctgggttg atggccggac
      781 cgttgattcc ctgacgacta cgagcacctg catgaagcag aaggcttcat ttggtgaccc
      841 cgacgtgata gttagggaat agtggtcggc cacagacggc gtggcgatcc tgtctccatc
      901 cgtctcgtct atcgggaggc gagttcgatg accctggtgg agggggctgc ggcttaggga
      961 ggcagaagct gagtaccgtc ggagggagct ccagggcccg gagcgactga cccctgccga
     1021 gaactcagag ggtcgtcgga agacggagag tgagcccgac gaccacccca ggcacgtctt
     1081 tggtcggcct gcggatcaag catggaagcc gtcattaagg tgatttcgtc cgcgtgtaaa
     1141 acctattgcg ggaaaatctc tccttctaag aaggaaatag gggccatgtt gtccctgtta
     1201 caaaaggaag ggttgcttat gtctccctca gatttatatt ctccggggtc ctgggatccc
     1261 atcactgcgg cgctctccca gcgggcaatg gtacttggaa aatcgggaga gttaaaaacc
     1321 tggggattgg ttttgggggc attgaaggcg gctcgagagg aacaggttac atctgagcaa
     1381 gcaaagtttt ggttgggatt agggggaggg agggtctctc ccccaggtcc ggagtgcatc
     1441 gagaaaccag ctacggagcg gcgaatcgac aaaggggagg aggtgggaga aacaactgtg
     1501 cagcgagatg cgaagatggc gccagaggaa gcggccacac ctaaaaccgt tggcacatcc
     1561 tgctatcatt gcggaacagc tgttggctgc aattgcgcca ccgccacagc ctcggcccct
     1621 cctccccctt atgtggggag tggtttgtat ccttccctgg cgggggtggg agagcagcag
     1681 ggccagggag ataacacgtc tcggggggcg gagcagccaa gggaggagcc agggcacgcg
     1741 ggtcaggccc ctgggccggc cctgactgac tgggcaaggg taagggagga gcttgcgagt
     1801 actggtccgc ccgtggtggc catgcctgta gtgattaaga cagagggacc cgcctggacc
     1861 cctctggagc caaaattgat cacaagactg gctgatacgg tcaggaccaa gggcttacga
     1921 tccccgatca ctatggcaga agtggaagcg ctcatgtcct ccccgttgct gccgcatgac
     1981 gtcacgaatc taatgagagt gattttagga cctgccccat atgccttatg gatggacgct
     2041 tggggagtcc aactccagac ggttatagcg gcagccactc gcgacccccg acacccagcg
     2101 aacggtcaag ggcgggggga acggactaac ttggatcgat taaagggctt agctgatggg
     2161 atggtgggca acccacaggg tcaggccgca ttattaagac cgggggaatt ggttgctatt
     2221 acggcgtcgg ctctccaggc gtttagagaa gttgcccggc tggcggaacc tgcaggtcca
     2281 tgggcggaca tcacgcaggg accatctgag tcctttgttg attttgccaa tcggcttata
     2341 aaggcggttg aggggtcaga tctcccgcct tccgcgcggg ctccggtgat cattgactgc
     2401 tttaggcaga agtcacagcc agatattcag cagcttatac gggcagcacc ctccacgctg
     2461 accaccccag gagagataat caaatatgtg ctagacaggc agaagattgc ccctcttacg
     2521 gatcaaggca tagccgcggc catgtcgtct gctatccagc ccttagttat ggcagtagtc
     2581 aatagagaga gggatggaca aactgggtcg ggtggtcgtg cccgagggct ctgctacact
     2641 tgtggatccc cgggacatta tcaggcacag tgcccgaaaa aacgaaagtc aggaaacagc
     2701 cgtgagcgat gtcagctgtg tgacgggatg ggacacaacg ctaaacagtg taggaagcgg
     2761 gatggcaacc agggccaacg cccaggaaga ggtctctctt cggggccgtg gcccggccct
     2821 gagcagcctg ccgtctcgtt agcgatgaca atggaacata aagatcgccc cttggttagg
     2881 gtcattctga ctaacactgg gagtcatcca gtcaaacaac gttcggtgta tatcaccgcg
     2941 ctgttggact ccggagcgga catcactatt atttcggagg aggattggcc tactgattgg
     3001 ccggtggtgg acaccgcgaa cccacagatc catggcatag gagggggaat tcccatgcga
     3061 aaatcccggg atatgataga ggtgggggtt attaaccgag acgggtcgtt ggagcgaccc
     3121 ctgctcctct tccccgcagt cgctatggtt agagggagta tcctaggaag agattgtctg
     3181 cagggcctag ggctccgctt gacaaattta tagggagggc cactgttctt actgttgcgc
     3241 tacatctggc tattccgctc aaatggaagt cagaccgcac gcctgtgtgg attgaccagt
     3301 ggccccttcc tgaaggtaaa cttgtagcgc taacgcaatt agtggaaaaa gaattacagt
     3361 taggacatat agagccctca cttagttgtt ggaacacacc tgtttttgtg atccggaagg
     3421 cttccgggtc ttatcgctta ttgcatgatt tgcgcgctgt taacgccaag cttgttcctt
     3481 ttggggccgt ccaacagggg gcgccagttc tctccgcgct cccgcgtggc tggcccctga
     3541 tggtcctaga cctcaaggat tgcttctttt ctatccctct tgcggaacaa gatcgcgaag
     3601 cttttgcatt tacgctcccc tctgtgaata accaggcccc cgctcgaaga ttccaatgga
     3661 aggtcttgcc ccaagggatg acctgttctc ccactatctg tcagttggta gtgggtcagg
     3721 tgctcgagcc cttgcgactc aagcacccag ctctgcgcat gttgcattat atggacgatc
     3781 ttttgctagc cgcctcaagt catgatgggt tggaagcggc agggaaggag gttatcggta
     3841 cattggaaag agccgggttc actatttcgc cggataagat ccagagggag cccggagtac
     3901 aatatcttgg gtacaagtta ggcagtacgt atgtagcacc cgtaggcttg gtagcagaac
     3961 ccaggatagc caccttgtgg gatgttcaaa agctggtggg gtcacttcag tggcttcgcc
     4021 cagcgttagg gatcccgcca cgactgatgg gtccctttta tgagcagtta cgagggtcag
     4081 atcctaacga ggcgagggaa tggaatctag acatgaaaat ggcctggaga gagatcgtac
     4141 agcttagcac tactgctgcc ttggaacgat gggaccctgc ccagcctctg gaaggagcgg
     4201 tcgctagatg tgaacagggg gcaatagggg tcctgggaca gggactgtcc acacacccaa
     4261 ggccatgttt gtggttattc tccacccaac ccaccaaggc gtttactgct tggttagaag
     4321 tgctcaccct tttgattact aagctacgcg cttcggcagt gcgaaccttt ggcaaggagg
     4381 ttgatatcct cctgttgcct gcatgcttcc gggaggacct tccgctcccg gaggggatcc
     4441 tgttagcact tagggggttt gcaggaaaaa tcaggagtag tgacacgcca tctatttttg
     4501 acattgcgcg tccactgcat gtttctctga aagtgagggt taccgaccac cctgtgccgg
     4561 gacccactgt ctttaccgac gcctcctcaa gcacccataa aggggtggta gtctggaggg
     4621 agggcccaag gtgggagata aaagaaatag ttgatttggg ggcaagtgta caacaactgg
     4681 aggcacgcgc tgtggccatg gcacttctgc tgtggccgac aacgcccact aatgtagtga
     4741 ctgactctgc gtttgttgcg aaaatgttac tcaagatggg acaggaggga gtcccgtcta
     4801 cagcggcagc ttttatttta gaggatgcgt taagccaaag gtcagccatg gccgccgttc
     4861 tccacgtgcg gagtcattct gaagtgccag ggtttttcac agaaggaaat gacgtggcag
     4921 atagccaagc cacctttcaa gcgtatccct tgagagaggc taaagatctt cataccgctc
     4981 tccatattgg accccgcgcg ctatccaaag cgtgtaatat atctatgcag caggctaggg
     5041 aggttgttca gacctgcccg cattgtaatt cagcccctgc gttggaggcc ggggtaaacc
     5101 ctaggggttt gggaccccta cagatatggc agacagactt tacgcttgag cctagaatgg
     5161 ctccccgttc ctggctcgct gttactgtgg acaccgcctc atcagcgata gtcgtaactc
     5221 agcatggccg tgttacatcg gttgctgcac aacatcattg ggccacggct atcgccgttt
     5281 tgggaagacc aaaggccata aaaacagata acgggtcctg cttcacgtct aaatccacgc
     5341 gggagtggct cgcgagatgg gggatagcac acaccaccgg gattccggga aattcccagg
     5401 gtcaagctat ggtagagcgg gccaaccggc tcctgaaaga taagatccgt gtgcttgcgg
     5461 aaggggacgg ctttatgaaa agaatccccg ccagcaaaca gggggaacta ctagccaaag
     5521 caatgtatgc cctcaatcac tttgagcgtg gtgaaaacac aaaaacaccg gtacaaaaac
     5581 actggagacc taccgttctt acagaaggac ccccggttaa aatacgaata gagacagggg
     5641 agtgggaaaa aggatggaac gtgctggtct ggggacgagg ttatgccgct gtgaaaaaca
     5701 gggacactga taaggttatt tgggtaccct ctcggaaagt taaaccggat gtcacccaaa
     5761 aggatgaggt gactaagaaa gatgaggcga gccctctttt tgcaggcatt tctgactgga
     5821 taccctggga agacgagcaa gaaggactcc aaggagaaac cgctagcaac aagcaagaaa
     5881 gacccggaga agacaccctt gctgccaacg agagttaatt atattctcat tattggtgtc
     5941 ctggtcttgt gtgaggttac gggggtaaga gctgatgtcc acttactcga gcagccaggg
     6001 aacctttgga ttacatgggc caaccgtaca ggccaaacgg atttttgcct ctctacacag
     6061 tcagccacct ccccttttca aacatgtttg ataggtatcc cgtcccctat ttccgagggt
     6121 gattttaagg gatatgtttc tgatacaaat tgcaccacct tgggaactga tcggttagtc
     6181 tcgtcagccg actttactgg cggacctgac aacagtacca ccctcactta tcggaaggtc
     6241 tcatgcttgt tgttaaagct gaatgtctct atgtgggatg agccacctga actacagctg
     6301 ttaggttccc agtctctccc taacattact aatattgctc agatttccgg tataaccggg
     6361 ggatgcgtag gcttcagacc acaaggggtt ccttggtatc taggttggtc tagacaggag
     6421 gccacgcggt ttctccttag acacccctct ttctctaaat ccacggaacc gtttacggtg
     6481 gtgacagcgg ataggcacaa tctttttatg gggagtgagt actgcggtgc atatggctac
     6541 agattttgga acatgtataa ctgctcacag gtggggcggc agtaccgctg tggtaatgcg
     6601 cgcagccccc gcccgggtct tcctgaaatc cagtgtacaa ggagaggagg caaatgggtt
     6661 aatcaatcac aggaaattaa tgagtcggag ccgttcagct ttacggtgaa ctgtacagct
     6721 agtagtttgg gtaatgccag tgggtgttgc ggaaaagcag gcacgattct cccgggaaag
     6781 tgggtcgaca gcacacaagg tagtttcacc aaaccaaaag cgctaccacc cgcaattttc
     6841 ctcatttgtg gggatcgcgc atggcaagga attcccagtc gtccggtagg gggcccctgc
     6901 tatttaggca agcttaccat gttagcacct aagcatacag atattctcaa ggtgcttgtc
     6961 aattcatcgc ggacaggtat aagacgtaaa cgaagcacct cacacctgga tgatacatgc
     7021 tcagatgaag tgcagctttg gggtcctaca gcaagaatct ttgcatctat cctagccccg
     7081 ggggtagcag ctgcgcaagc cttaagagaa attgagagac tagcctgttg gtccgttaaa
     7141 caggctaact tgacaacatc actcctcggg gacttattgg atgatgtcac gagtattcga
     7201 cacgcggtcc tgcagaaccg agcggctatt gacttcttgc tcctagctca cggccatggc
     7261 tgtgaggacg ttgccggaat gtgctgtttc aatttgagtg atcacagtga gtctatacag
     7321 aagaagttcc agctaatgaa ggaacatgtc aataagatcg gcgtggacag cgacccaatt
     7381 ggaagttggc tgcgaggact attcggggga ataggagaat gggccgttca tttgctgaaa
     7441 ggactgcttt tggggcttgt agttattttg ttgctagtag tgtgcctgcc ttgccttttg
     7501 caaatcgtat gcggtaacat cagaaagatg attaataact ccatcagcta ccacacggaa
     7561 tataagaagc tgcaaaaggc ctgtgggcag cctgaaagca gaatagtata aggcagtaca
     7621 tgggtggtgg tatagcgctt gcgagtccat cgaggaaggc aggaaagaca gctattggta
     7681 attgtgaaat acgcttttgt ctgtgtgctg caggagctga gctgactctg ctggtggcct
     7741 cgcgtaccac tgtggcatcg ggccattacg gccgcggccg cctctagagg atctggtacc
     7801 acgcgtatcg ataagcttat ggggagcagc aagagcaagc ccaagtctag aatgaacgac
     7861 gtagccattg tgaaggaggg ctggctgcac aaacgagggg aatatattaa aacctggcgg
     7921 ccacgctact tcctcctcaa gaacgatggc acctttattg gctacaagga acggcctcag
     7981 gatgtggatc agcgagagtc cccactcaac aacttctcag tggcacaatg ccagctgatg
     8041 aagacagagc ggccaaggcc caacaccttt atcatccgct gcctgcagtg gaccacagtc
     8101 attgagcgca ccttccatgt ggaaacgcct gaggagcggg aagaatgggc caccgccatt
     8161 cagactgtgg ccgatggact caagaggcag gaagaagaga cgatggactt ccgatcaggc
     8221 tcacccagtg acaactcagg ggctgaagag atggaggtgt ccctggccaa gcccaagcac
     8281 cgtgtgacca tgaacgagtt tgagtacctg aaactactgg gcaagggcac ctttgggaaa
     8341 gtgattctgg tgaaagagaa ggccacaggc cgctactatg ccatgaagat cctcaagaag
     8401 gaggtcatcg tcgccaagga tgaggttgcc cacacgctta ctgagaaccg tgtcctgcag
     8461 aactctaggc atcccttcct tacggccctc aagtactcat tccagaccca cgaccgcctc
     8521 tgctttgtca tggagtatgc caacgggggc gagctcttct tccacctgtc tcgagagcgc
     8581 gtgttctccg aggaccgggc ccgcttctat ggtgcggaga ttgtgtctgc cctggactac
     8641 ttgcactccg agaagaacgt ggtgtaccgg gacctgaagc tggagaacct catgctggac
     8701 aaggacgggc acatcaagat aacggacttc gggctgtgca aggaggggat caaggatggt
     8761 gccactatga agacattctg cggaacgccg gagtacctgg cccctgaggt gctggaggac
     8821 aacgactacg gccgtgcagt ggactggtgg gggctgggcg tggtcatgta tgagatgatg
     8881 tgtggccgcc tgcccttcta caaccaggac cacgagaagc tgttcgagct gatcctcatg
     8941 gaggagatcc gcttcccgcg cacactcggc cctgaggcca agtccctgct ctccgggctg
     9001 ctcaagaagg accctacaca gaggctcggt gggggctctg aggatgccaa ggagatcatg
     9061 cagcaccggt tctttgccaa catcgtgtgg caggatgtgt atgagaagaa gctgagccca
     9121 cctttcaagc cccaggtcac ctctgagact gacaccaggt atttcgatga ggagttcaca
     9181 gctcagatga tcaccatcac gccgcctgat caagatgaca gcatggagtg tgtggacagt
     9241 gagcggaggc cgcacttccc ccagttctcc tactcagcca gtggcacagc ctacccatac
     9301 gatgttccag attacgcttc tagatgaatt cacgcgtaag cttatcgatg tcgacgggcc
     9361 cggccgcctc ggcccgatgc gatgtacggg ccagatatac gcgtatctga ggggactagg
     9421 gtgtgtttag gcgaaaagcg gggcttcggt tgtacgcggt taggagtccc ctcaggatat
     9481 agtagtttcg cttttgcata gggaggggga aatgtagtct tatgcaatac tcttgtagtc
     9541 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     9601 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     9661 tctgacatgg attggacgaa ccactgaatt ccgcattgca gagatattgt atttaagtgc
     9721 ctagctcgat acaataaacg ccatttgacc attcaccaca ttggtgtgca cctgggttga
     9781 tggccggacc gttgattccc tgacgactac gagcacctgc atgaagcaga aggcttcatt
     9841 tggtgacccc gacgtgatag ttagggaata gtggtcggcc acagacggcg tggcgatcct
     9901 gtctccatcc gtctcgtcta tcgggaggcg agttcgatga ccctggtgga gggggctgcg
     9961 gcttagggag gcagaagctg agtaccgtcg gaggggatcc acaggacggg tgtggtcgcc
    10021 atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca
    10081 aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc
    10141 gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg
    10201 cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg
    10261 atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac
    10321 tgtgataaac taccgcatta aagctccaag cttggctgtt tcctgtgtga aattgttatc
    10381 cgctcacaat tccacacatt atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    10441 aatgagtgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
    10501 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
    10561 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    10621 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    10681 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    10741 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    10801 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    10861 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
    10921 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    10981 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    11041 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    11101 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    11161 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
    11221 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    11281 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    11341 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    11401 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    11461 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    11521 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    11581 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    11641 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    11701 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    11761 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    11821 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    11881 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    11941 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    12001 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    12061 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    12121 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    12181 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    12241 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    12301 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    12361 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    12421 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
    12481 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
    12541 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
    12601 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
    12661 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
    12721 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
    12781 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
    12841 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
    12901 gatgcctccg tgtaaggagg atttctgttc atgggggtaa tgataccgat gaaacgagag
    12961 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag
    13021 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc
    13081 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg
    13141 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca
    13201 cggaaaccga agaccattca tgttgttgct caggtcgcag
//
