LOCUS       sequence-383938-        3921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC29A1_STOP
FEATURES             Location/Qualifiers
     source          1..3921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1478..1577)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1594..1613)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1595..1613)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1618..1634)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1618..1634)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1736..1755)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1747..2556
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1814..1833)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2703..3291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3192..3211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3445..3462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3621..3648)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3740..3826)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3875..3897
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3889..3906
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3890..3906
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgac cacatcccac
      121 cagcctcagg accggtacaa ggccgtgtgg ctgatcttct ttatgctggg cctgggcacc
      181 ctgctgccat ggaacttctt tatgaccgcc acacagtatt tcaccaacag gctggacatg
      241 agccagaacg tgagcctggt gacagccgag ctgagcaagg atgcacaggc atccgccgca
      301 ccagcagcac cactgcctga gcgcaactcc ctgtctgcca tctttaacaa tgtgatgacc
      361 ctgtgcgcca tgctgcctct gctgctgttc acatacctga attcttttct gcaccagcgg
      421 atcccacagt ctgtgagaat cctgggcagc ctggtggcca tcctgctggt gttcctgatc
      481 accgccatcc tggtgaaggt gcagctggac gccctgcctt tctttgtgat cacaatgatc
      541 aagatcgtgc tgatcaattc tttcggcgcc atcctgcagg gaagcctgtt tggactggca
      601 ggactgctgc ctgcaagcta caccgcacca atcatgtccg gacagggact ggcaggcttc
      661 tttgcaagcg tggccatgat ctgtgccatc gcaagcggat ccgagctgtc tgagagcgcc
      721 tttggctatt tcatcaccgc ctgcgccgtg atcatcctga caatcatctg ttacctgggc
      781 ctgcccaggc tggagttcta tcgctactat cagcagctga agctggaggg ccctggcgag
      841 caggagacca agctggatct gatctccaag ggagaggagc caagggcagg caaggaggag
      901 tctggcgtgt ccgtgtctaa cagccagccc acaaatgagt cccactctat caaggccatc
      961 ctgaagaaca tctctgtgct ggccttcagc gtgtgcttca tctttaccat cacaatcggc
     1021 atgtttcccg ccgtgaccgt ggaggtgaag agctccatcg ccggctctag cacatgggag
     1081 cggtatttca tccctgtgag ctgttttctg accttcaata tctttgactg gctgggcaga
     1141 tccctgacag ccgtgttcat gtggcccggc aaggattcca ggtggctgcc ttctctggtg
     1201 ctggcaaggc tggtgtttgt gccactgctg ctgctgtgca acatcaagcc ccggagatac
     1261 ctgaccgtgg tgttcgagca cgatgcctgg tttatcttct ttatggccgc cttcgccttt
     1321 tccaatggct atctggcctc tctgtgcatg tgctttggcc caaagaaggt gaagccagca
     1381 gaggcagaga cagcaggagc catcatggcc ttcttcctgt gcctgggact ggccctggga
     1441 gccgtgttca gctttctgtt cagagccatc gtgtaagacc cagctttctt gtacaaagtt
     1501 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1561 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1621 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1681 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1741 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1801 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1861 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1921 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1981 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2041 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2101 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2161 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2221 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2281 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2341 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2401 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2461 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2521 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2581 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2641 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2821 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3301 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3361 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3421 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3481 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3541 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3601 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3661 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3721 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3781 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3841 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3901 gccagtctta agctcgggcc c
//
