LOCUS       sequence-383940-        5001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF280C transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF280C
FEATURES             Location/Qualifiers
     source          1..5001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    782..793
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(2880..2979)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2996..3015)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2997..3015)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3020..3036)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3020..3036)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3467..4258
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4351..4939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4840..4859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gatgacgaca aaccttttca accaaaaaac atttcaaaaa tggcagaact
      721 ctttatggaa tgtgaagaag aagagctaga gccatggcag aagaaagtag aagaaactca
      781 ggatgaggat gacgatgaac tgatctttgt tggagagata tcaagttcaa aaccagccat
      841 ttcaaatatt ttgaacagag gccactccag ctcatcttca aaaggaataa agagtgagcc
      901 acacagtcca ggtattcctg aaatattcag gactgcaagt caacgctgca gagacccacc
      961 atcaaatcca gtggctgcct cgcctagatt tcatcttgta tctaaatctt cacaaagctc
     1021 tgttactgtt gagaatgcgt ctaaacctga ttttacaaag aattcacaag ttggatcgga
     1081 taattcttca attttactgt ttgactcgac ccaggaatca ctaccaccat cccaagacat
     1141 accagcaatt tttagagaag gtatgaaaaa tacttcatat gtgttgaaac atccttctac
     1201 ttctaaagta aacagtgtta ctccaaaaaa accaaagacc agtgaagatg ttcctcagat
     1261 aaatccctcc acttcattgc ctttaattgg ctctcctcca gtgacatcct cccaagttat
     1321 gctgtcaaaa ggtacaaata cctcatctcc atatgatgct ggagcagatt acctaagagc
     1381 ttgtccaaag tgcaatgttc agttcaatct tttggatcct ttgaaatacc acatgaagca
     1441 ttgttgtcca gacatgataa ctaaattttt gggagtaatt gttaaatcag aacgtccatg
     1501 tgatgaagac aagactgatt cagagacagg aaagttgatc atgttagtca atgagtttta
     1561 ttatggaagg catgaaggag tcactgagaa agagccaaag acttacacaa cctttaaatg
     1621 cttcagttgc tcgaaagttc ttaaaaataa tattaggttt atgaaccaca tgaaacatca
     1681 cttggaactt gagaagcaga acaatgaaag ctgggaaaac cacaccacct gccagcactg
     1741 ttaccggcaa tatcccacac ccttccaact gcagtgccac attgagagta cacacactcc
     1801 ccatgagttt tctactattt gcaaaatctg tgaattatca tttgaaacag agcatattct
     1861 tttacaacat atgaaggaca cccataaacc tggtgaaatg ccatatgttt gccaggtttg
     1921 ccagtttaga tcatcaacat tttctgatgt agaagctcat tttagagcag cccatgaaaa
     1981 cactaagaac ttgctatgtc cattttgcct caaagttagt aaaatggcaa ccccctacat
     2041 gaatcattac atgaagcatc agaaaaaagg agttcatcgt tgcccaaaat gcagactaca
     2101 atttttgacc agcaaggaga aagctgaaca taaggcgcag catcgtacat ttataaagcc
     2161 taaagaacta gaaggattgc ctcctggagc aaaagttact attcgagctt cacttggacc
     2221 tctccagtca aaattaccaa ctgcaccttt cggttgcgct ccaggcactt cttttcttca
     2281 ggtcacacct ccgacatctc aaaatacaac tgctagaaat cctagaaaat ctaatgccag
     2341 tagatctaag acaagtaagc ttcatgcaac tacatccact gcaagtaaag ttaatacaag
     2401 taagccaagg ggacgtatag ctaagtccaa agcaaaaccc tcttacaagc aaaagcgaca
     2461 gcgcaacaga aaaaataaaa tgagccttgc tttgaagaac ataaggtgtc gtcggggcat
     2521 tcacaagtgc attgaatgtc attccaaaat aaaagatttt gcaagccact tttctatata
     2581 catccactgc agtttttgca agtacaatac taactgtaac aaagcctttg taaatcatat
     2641 gatgagctct catagcaacc atccaggtaa acggttttgt attttcaaga agcattcagg
     2701 aactctcagg ggcattactc tagtgtgcct taaatgtgat ttcctagctg attcttccgg
     2761 cttagatcgt atggctaaac acttaagtca acgtaaaact catacttgcc aagttataat
     2821 agagaatgtt tccaaaagta cctcaacttc tgaacccact actggctgct cattgaaata
     2881 cccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     2941 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     3001 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     3061 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca
     3121 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
     3181 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     3241 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     3301 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     3361 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     3421 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     3481 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     3541 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     3601 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     3661 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     3721 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     3781 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     3841 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     3901 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     3961 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     4021 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     4081 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     4141 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     4201 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     4261 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     4321 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4381 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4441 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4501 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4561 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4621 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4681 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4741 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4801 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4861 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4921 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4981 ctggcctttt gctcacatgt t
//
