LOCUS       sequence-383943-        8564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UCK2 overexpression vector with blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Girish V, Lakhani AA, Thompson SL, Scaduto CM, Brown LM, Hagenson 
            RA, Sausville EL, Mendelson BE, Kandikuppa PK, Lukow DA, Yuan ML, 
            Stevens EC, Lee SN, Schukken KM, Akalu SM, Vasudevan A, Zou C, 
            Salovska B, Li W, Smith JC, Taylor AM, Martienssen RA, Liu Y, Sun R,
            Sheltzer JM
  TITLE     Oncogene-like addiction to aneuploidy in human cancers.
  JOURNAL   Science. 2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521.
  PUBMED    37410869
REFERENCE   2  (bases 1 to 8564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521."
COMMENT     Sequence Label: pLV-Bsd-CMV-hUCK
FEATURES             Location/Qualifiers
     source          1..8564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      763..772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(773..797)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(818..1021)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(847..867)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1022..1325
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(1386..1406)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    1424..1541
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1807..1851)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2036..2269
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2762..2887
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2934..3114
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3115..3341)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(3369..3389)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3369..3387)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3408..3424)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3408..3424)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3421..3443)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3432..3448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3456..3462,3463..3480,3481..3486))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3501..3522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3639..3656)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3810..4398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3890..3909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4569..5360,5361..5429))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5192..5211
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5430..5534)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5560..6015)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5697..5718)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5909..5928
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6142..6164
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6156..6173
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6157..6173
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6183..6202
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6183..6201
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6222..6357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6397..6518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6408..6427)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6462..6481
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             6590..6823
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(6910..7308)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(7327..7374)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7384..7883)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7432..7451)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7825..7848
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    7914..8502
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8020..8031
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8429..8449
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    8520..8544
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actccgatgc ctgcctcttg cgtgaagggg tgtagccgtt gagacagcca ttggtctgcc
       61 gtttggaggg ccctccattc aggatgtcct ggatgtgctg cacgatgagg ttgatggcca
      121 ccagattatc tgcacctcta gggatgatca catcagcata cttctttgtt ggcaagcaga
      181 attcctcaaa ggcaggcttg acgaacgtaa tgtactgaga taaaatctgc tcaagatccc
      241 tgcctctctc gctgatgtcc cttaatactc tgcgtgagag ccgggtgtcc gcatctgtat
      301 ccacaaaaag cttcatctgg aacaggtctc gtacctcctg ggagtagaag gccaggatcc
      361 cttcaaagag caccacgtct gcgggataga cagtaactgt ctcctccttc cgggaatggg
      421 agacaaagtc atacacgggg atctggactg ttttcccttc agtgatttct ttgagtgttt
      481 tgagaatgag ttcattgtca aaggcatccg ggtggtcaaa gttgaactgg cccttcaggg
      541 ctttggcctt ctgctccgag gtaaggacac ggtagaagct atcctggctc aggatgacca
      601 cctgcttctg gcgatagtcc acctcattct gccccaggag ctgcacgatc ttagcacaca
      661 cggaagactt gccgctagct gttcccccgc tgacgcctat aaggaagggc tcgccgccgt
      721 tgggctgctg gtggttctgc agggtctgct cgctgtcccc ggccatggtg gcagcctgct
      781 tttttgtaca aacttggatc tgacggttca ctaaaccagc tctgcttata tagacctccc
      841 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
      901 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
      961 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     1021 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     1081 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     1141 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     1201 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     1261 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     1321 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     1381 aactacaact tttctataca aagttgatcc gataatcact agtaaaattt tgaatttttg
     1441 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
     1501 cccctgcact gtacccccca atcccccctt ttcttttaaa agctagcgat accgtcgaga
     1561 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
     1621 ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
     1681 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
     1741 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
     1801 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
     1861 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
     1921 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
     1981 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
     2041 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
     2101 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
     2161 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
     2221 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
     2281 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
     2341 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
     2401 tccaggtctg aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt
     2461 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
     2521 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
     2581 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa
     2641 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
     2701 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
     2761 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
     2821 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
     2881 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
     2941 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     3001 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     3061 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccgtttat
     3121 gtatcgagct aggcacttaa atacaatatc tctgcaatgc ggcaattcag tggttcgtcc
     3181 aatccatgtc agacccgtct gttgccttcc taataaggca cgatcgtacc accttacttc
     3241 caccaatcgg catgcacggt gctttttctc tccttgtaag gcatgttgct aactcatcgt
     3301 taccatgttg caagactaca agagtattgc ataagactac attaagcttg cagctccagc
     3361 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     3421 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     3481 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     3541 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3601 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     3661 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3721 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3781 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3841 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3901 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3961 tacctgtccg cctttctctc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4021 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4081 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4141 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4201 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4261 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4321 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4381 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4441 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4501 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4561 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4621 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4681 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4741 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4801 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4861 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4921 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4981 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5041 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     5101 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     5161 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5221 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5281 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5341 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5401 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5461 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5521 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     5581 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     5641 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     5701 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     5761 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     5821 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     5881 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     5941 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6001 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     6061 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     6121 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
     6181 cgtaatacga ctcactatag ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc
     6241 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     6301 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     6361 cggagttagg ggcgggatag ctagagccag acatgataag atacattgat gagtttggac
     6421 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     6481 ctttatttgt aaccattata agctgcaata aacaagttcc tctcactctc tgatattcat
     6541 ttctttgcaa gttataaata ctgaataata agatgacatg aactactact gctagagatt
     6601 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     6661 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     6721 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca
     6781 aaaagcagat cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt
     6841 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg taccgagctc
     6901 gaattgtgct tagccctccc acacataacc agagggcagc aattcacgaa tcccaactgc
     6961 cgtcggctgt ccatcactgt ccttcactat ggctttgatc ccaggatgca gatcgagaag
     7021 cacctgtcgg caccgtccgc aggggctcaa gatgcccctg ttctcatttc cgatcgcgac
     7081 gatacaagtc aggttgccag ctgccgcagc agcagcagtg cccagcacca cgagttctgc
     7141 acaaggtccc ccagtaaaat gatatacatt gacaccagtg aagatgcggc cgtcgctaga
     7201 gagagctgcg ctggcgacgc tgtagtcttc agagatgggg atgctgttga ttgtagccgt
     7261 tgctctttca atgagggtgg attcttcttg agacaaaggc ttggccatgg tttagttcct
     7321 caccttgtcg tattatacta tgccgatata ctatgccgat gattaattgt caacacgtga
     7381 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
     7441 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
     7501 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
     7561 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
     7621 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
     7681 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
     7741 accaaagaac ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag
     7801 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
     7861 tgggaaaagc gcctccccta cccggtagaa ttcgaaccgc gggaattccc gatgcgggga
     7921 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg
     7981 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
     8041 gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc atggaaagga
     8101 cgtcagcttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
     8161 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
     8221 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
     8281 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
     8341 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
     8401 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
     8461 accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttatcggaat tcgattatca
     8521 ccactttgta caagaaagct gggttcaatg cggcctgctg ctgg
//
