LOCUS       sequence-383957-       10648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-ON 3rd generation expression vector with inducible expression of
            MDM4 and a puromycin selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Girish V, Lakhani AA, Thompson SL, Scaduto CM, Brown LM, Hagenson 
            RA, Sausville EL, Mendelson BE, Kandikuppa PK, Lukow DA, Yuan ML, 
            Stevens EC, Lee SN, Schukken KM, Akalu SM, Vasudevan A, Zou C, 
            Salovska B, Li W, Smith JC, Taylor AM, Martienssen RA, Liu Y, Sun R,
            Sheltzer JM
  TITLE     Oncogene-like addiction to aneuploidy in human cancers.
  JOURNAL   Science. 2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521.
  PUBMED    37410869
REFERENCE   2  (bases 1 to 10648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521."
COMMENT     Sequence Label: pLVX-TetOne-Puro-MDM4
FEATURES             Location/Qualifiers
     source          1..10648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..378
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    19..37
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    55..73
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    91..109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    127..145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    163..181
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    199..217
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    235..253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    2053..2187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2231..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(2609..2653)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2838..3071
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3568..3693
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3740..4373
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4541..4675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4723..4827
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4828..4896,4897..5688)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5046..5065)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5859..6447
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6348..6367
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6601..6618
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6735..6756
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6771..6776,6777..6794,6795..6801)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6809..6825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6814..6836
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6833..6849
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6833..6849
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             6977..7610
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7817..8405
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7923..7934
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8332..8352
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8419..9018)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8502..8522)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8999..9018
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(9027..9356)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9041..9176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(9367..10113)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(10132..10642)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10195..10213)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ttatcacctc gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta
       61 tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga
      121 gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag
      181 aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta
      241 tcagtgatag agaacgtata agctttgctt atgtaaacca gggcgcctat aaaagagtgc
      301 tgattttttg agtaaacttc aattccacaa cacttttgtc ttataccaac tttccgtacc
      361 acttcctacc ctcgtaaaga attcggcatg acatcatttt ccacctctgc tcagtgttca
      421 acatctgaca gtgcttgcag gatctctcct ggacaaatca atcaggtacg accaaaactg
      481 ccgcttttga agattttgca tgcagcaggt gcgcaaggtg aaatgttcac tgttaaagag
      541 gtcatgcact atttaggtca gtacataatg gtgaagcaac tttatgatca gcaggagcag
      601 catatggtat attgtggtgg agatcttttg ggagaactac tgggacgtca gagcttctcc
      661 gtgaaagacc caagccctct ctatgatatg ctaagaaaga atcttgtcac tttagccact
      721 gctactacag atgctgctca gactctcgct ctcgcacagg atcacagtat ggatattcca
      781 agtcaagacc aactgaagca aagtgcagag gaaagttcca cttccagaaa aagaactaca
      841 gaagacgata tccccacact gcctacctca gagcataaat gcatacattc tagagaagat
      901 gaagacttaa ttgaaaattt agcccaagat gaaacatcta ggctggacct tggatttgag
      961 gagtgggatg tagctggcct gccttggtgg tttttaggaa acttgagaag caactataca
     1021 cctagaagta atggctcaac tgatttacag acaaatcagg atgtgggtac tgccattgtt
     1081 tcagatacta cagatgactt gtggtttttg aatgagtcag tatcagagca gttaggtgtt
     1141 ggaataaaag ttgaagctgc tgatactgaa caaacaagtg aagaagtagg gaaagtaagt
     1201 gacaaaaagg tgattgaagt gggaaaaaat gatgacctgg aggactctaa gtccttaagt
     1261 gatgataccg atgtagaggt tacctctgag gatgagtggc agtgtactga atgcaagaaa
     1321 tttaactctc caagcaagag gtactgtttt cgttgttggg ccttgaggaa ggattggtat
     1381 tcagattgtt caaagttaac ccattctctc tccacgtctg atatcactgc catacctgaa
     1441 aaggaaaatg aaggaaatga tgtccctgat tgtcgaagaa ccatttcggc tcctgtcgtt
     1501 agacctaaag atgcgtatat aaagaaagaa aactccaaac tttttgatcc ctgcaactca
     1561 gtggaattct tggatttggc tcacagttct gaaagccaag agaccatctc aagcatggga
     1621 gaacagttag ataacctttc tgaacagaga acagatacag aaaacatgga ggattgccag
     1681 aatctcttga agccatgtag cttatgtgag aaaagaccac gagacgggaa cattattcat
     1741 ggaaggacgg gccatcttgt cacttgtttt cactgtgcca gaagactaaa gaaggctggg
     1801 gcttcatgcc ctatttgcaa gaaagagatt cagctggtta ttaaggtttt tatagcataa
     1861 accggtgccg gcggatccag accacctccc ctgcgagcta agctggacag ccaatgacgg
     1921 gtaagagagt gacatttttc actaacctaa gacaggaggg ccgtcagagc tactgcctaa
     1981 tccaaagacg ggtaaaagtg ataaaaatgt atcactccaa cctaagacag gcgcagcttc
     2041 cgagggattt gagatccaga catgataaga tacattgatg agtttggaca aaccaaaact
     2101 agaatgcagt gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc cttatttgta
     2161 accattataa gctgcaataa acaagttaag tcgataaact ggatctctgc tgtccctgta
     2221 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
     2281 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
     2341 tttaaaggcg ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct
     2401 ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg
     2461 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     2521 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     2581 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     2641 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     2701 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     2761 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     2821 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     2881 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     2941 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
     3001 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     3061 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     3121 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     3181 aattgtccct catatctcct cctccaggtc tgaagatcag cggccggccg cttgctgtgc
     3241 ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
     3301 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
     3361 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
     3421 tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac
     3481 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
     3541 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
     3601 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
     3661 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
     3721 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
     3781 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     3841 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     3901 tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag
     3961 ggctcgccac tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag
     4021 tcccttgtag caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc
     4081 cacgtgatga aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc
     4141 atccatccca tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc
     4201 ttctacctta tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata
     4261 tctgacccct ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta
     4321 gatccacaga tcaaggatat cttgtcttct ttgggagtga attagccctt ccaactacta
     4381 agtttgtagt acatatttaa caaatacaat ttctttaaaa tgaaaataat tcagaggaat
     4441 cacaggttta gagtaaatga aaccacaggt aattggcagt ggtaataggg tatggggtgg
     4501 gaagtttggg atgattttgg ttagcttgag ttatccagtt gatccagaca tgataagata
     4561 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     4621 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttgatct
     4681 cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4741 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4801 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4861 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4921 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4981 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5041 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5101 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5161 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5221 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5281 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5341 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5401 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5461 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5521 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5581 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5641 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5701 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5761 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5821 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5881 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5941 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6001 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6061 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6121 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6181 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6241 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6301 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6361 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6421 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6481 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6541 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6601 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6661 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6721 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6781 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6841 agctatgacc atgattacgc caagctctag ctagaggtcg acggtaaacg ctagcaatgt
     6901 caaggcctct cactctctga tattcatttc tttgcaagtt ataaatactg aataataaga
     6961 tgacatgaac tactactgct agagattttc cacactgact aaaagggtct gagggatctc
     7021 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     7081 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     7141 tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc
     7201 tcgccactcc ccagtcccgc ccaggccacg cctccctgga aagtccccag cggaaagtcc
     7261 cttgtagcaa gctcgatatc agcagttctt gaagtactcc ggatgcagct ctcgggccac
     7321 gtgatgaaat gctaggcggc tgtcaaacct ccactctaac acttctctct ccgggtcatc
     7381 catcccatgc aggctcacag ggtgtaacaa gctggtgttc tctcctttat tggcctcttc
     7441 taccttatct ggctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct
     7501 gacccctggc cctggtgtgt agttctgcta atcagggaag tagccttgtg tgtggtagat
     7561 ccacagatca aggatatctt gtcttcgttg ggagtgaatt agcccttcca gtcccctctt
     7621 ttcttttaaa aagtggctaa gatctacagc tgccttgtaa gtcattggtc ttaaaggtac
     7681 ctgaggtgtg actggaaaac ccacctcctc ctcctcttgt gcttctagcc aggcacaatc
     7741 agcattggta gctgctgtat tgctacttgt gattgctcca tgtttttcta ggtctcgact
     7801 gcagaattaa ttccaggcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga
     7861 aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct
     7921 ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca
     7981 caggcgagca gccatggaaa ggacgtcagc ttccccgaca acaccacgga attgtcagtg
     8041 cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg
     8101 gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc
     8161 caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg
     8221 ggccacaact cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa
     8281 gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta
     8341 aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta atccagaggt
     8401 tgattgttcc agacgcgttc aggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc
     8461 ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga aggggaggtt
     8521 gcggggcgcg gaggtctcca ggaaggcggg caccccggcg cgctcggccg cctccactcc
     8581 ggggagcacg acggcgctgc ccagaccctt gccctggtgg tcgggcgaga cgccgacggt
     8641 ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg
     8701 ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc gggccgatct cggcgaacac
     8761 cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc
     8821 gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt gcagctcggt
     8881 gacccgctcg atgtggcggt ccgggtcgac ggtgtggcgc gtggcggggt agtcggcgaa
     8941 cgcggcggcg agggtgcgta cggcccgggg gacgtcgtcg cgggtggcga ggcgcaccgt
     9001 gggcttgtac tcggtcatgg tcgcgttttg caaaagccta ggcctccaaa aaagcctcct
     9061 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     9121 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     9181 ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg
     9241 cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt
     9301 tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc
     9361 gcgcgtttac ccggggagca tgtcaaggtc aaaatcgtca agagcgtcag caggcagcat
     9421 atcaaggtca aagtcgtcaa gggcatcggc tgggagcatg tctaagtcaa aatcgtcaag
     9481 ggcgtcggtc ggcccgccgc tttcgcactt tagctgtttc tccaggccac atatgattag
     9541 ttccaggccg aaaaggaagg caggttcggc tccctgccgg tcgaacagct caattgcttg
     9601 tttcagaagt gggggcatag aatcggtggt aggtgtctct ctttcctctt ttgctacttg
     9661 atgctcctgt tcctccaata cgcagcccag tgtaaagtgg cccacggcgg acagagcgta
     9721 cagtgcgttc tccagggaga agccttgctg acacaggaac gcgagctgat tttccagggt
     9781 ttcgtactgt ttctctgttg ggcgggtgcc gagatgcact ttagccccgt cgcgatgtga
     9841 gaggagagca cagcggtatg acttggcgtt gttccgcaga aagtcttgcc atgactcgcc
     9901 ttccaggggg caggagtggg tatgatgcct gtccagcatc tcgattggca gggcatcgag
     9961 cagggcccgc ttgttcttca cgtgccagta cagggtaggc tgctcaactc ccagcttttg
    10021 agcgagtttc cttgtcgtca ggccttcgat accgactcca ttgagtaatt ccagagcaga
    10081 gtttatgact ttgctcttgt ccagtctaga catggtaatt cgatgatccc cctggggaga
    10141 gaggtcggtg attcggtcaa cgagggagcc gactgccgac gtgcgctccg gaggcttgca
    10201 gaatgcggaa caccgcgcgg gcaggaacag ggcccacact accgccccac accccgcctc
    10261 ccgcaccgcc ccttcccggc cgctgctctc ggcgcgccct gctgagcagc cgctattggc
    10321 cacagcccat cgcggtcggc gcgctgccat tgctccctgg cgctgtccgt ctgcgagggt
    10381 actagtgaga cgtgcggctt ccgtttgtca cgtccggcac gccgcgaacc gcaaggaacc
    10441 ttcccgactt aggggcggag caggaagcgt cgccgggggg cccacaaggg tagcggcgaa
    10501 gatccgggtg acgctgcgaa cggacgtgaa gaatgtgcga gacccagggt cggcgccgct
    10561 gcgtttcccg gaaccacgcc cagagcagcc gcgtccctgc gcaaacccag ggctgccttg
    10621 gaaaaggcgc aaccccaacc ccgtggaa
//
