LOCUS       sequence-383960-       10382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of mouse Gata2 without the 
            N-terminal zinc finger (amino acids 287-342), in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Silverio-Alves R, Kurochkin I, Rydstrom A, Vazquez Echegaray C, 
            Haider J, Nicholls M, Rode C, Thelaus L, Lindgren AY, Ferreira AG, 
            Brandao R, Larsson J, de Bruijn MFTR, Martin-Gonzalez J, Pereira CF
  TITLE     GATA2 mitotic bookmarking is required for definitive haematopoiesis.
  JOURNAL   Nat Commun. 2023 Aug 14;14(1):4645. doi: 10.1038/s41467-023-40391-x.
  PUBMED    37580379
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-14"; volume: "14(1):4645. doi"; pages: " 
            10.1038/s41467-023-40391-x"
COMMENT     Sequence Label: pFUW-tetO-mCh-Gata2-del-N-ZF(287-342)
FEATURES             Location/Qualifiers
     source          1..10382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2724
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2721..2745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2842..3543
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2975..2993)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3243..3262
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3437..3460
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4843..5431
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4896..4916)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5314..5325)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5434..5450)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5435..5451)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5956..6136
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6162..6179)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6168..6392
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6438..6866
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6525..6544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6735..6756
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6875..6895)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6880..7209
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7060..7195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7122..7141
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7257..7304
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7323..7697
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7827..7948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7864..7883)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7918..7937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7997..8013)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7997..8013)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8010..8032)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8021..8037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8045..8051,8052..8069,8070..8075))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8090..8111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8228..8245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8399..8987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8479..8498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9158..9949,9950..10018))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9781..9800
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10019..10123)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10198..10217)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
     2701 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2761 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa
     2821 ttcaccggtt ctagagctag catggtgagc aagggcgagg aggtcatcaa agagttcatg
     2881 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     2941 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     3001 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
     3061 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
     3121 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
     3181 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
     3241 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     3301 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
     3361 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
     3421 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     3481 cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac
     3541 aaggaggtgg cgcctgagca gcctcgctgg atggcgcacc ccgccgtatt gaatgcgcag
     3601 caccccgact cgcaccatcc gggcctggcg cataactaca tggagccagc acagctgctg
     3661 cctcccgacg aggtggatgt cttcttcaac catctcgact cgcagggcaa cccttactac
     3721 gccaacccgg cccacgcgcg cgcgcgcgtt tcctacagcc cggcgcatgc ccgtctcacc
     3781 ggaggccaga tgtgccgacc acacttgttg cacagcccag gcttgccgtg gctggacggg
     3841 ggcaaagcag ctctctctgc cgccgctgcc catcaccaca gtccctggac cgtcagcccg
     3901 ttctccaaga ccccgctgca cccctcagct gctggagcac ccggagggcc tctgtctgtt
     3961 tacccagggg ctgcgggtgg gagcggggga ggcagtggga gctccgtggc ctccctcacc
     4021 cccactgcag cccactcggg ctcccatctc ttcggcttcc cacccacgcc acccaaagaa
     4081 gtgtctccag accccagcac aacaggagct gcttccccgg cctcttcttc tgcagggggt
     4141 agtgtagccc ggggtgagga caaggatggc gtcaagtacc aagtgtcact ctccgagagc
     4201 atgaagatgg aaggcggcag tcccctgcgc ccgggcctag ctaccatggg cacccagcct
     4261 gcaacacacc acccgatacc cacctatccc tcctatgtgc ccgccgcaac tcatgactat
     4321 ggcagcagtc tcttccatcc aggaggcttc ctgggtggcc ccgcctccag cttcacccct
     4381 aagcagagaa gcaaggctcg cagaagagcg ggcacctgtt gtgcaaattg tcagacgaca
     4441 accaccacct tatggcgccg gaacgccaac ggggaccctg tgtgcaacgc ctgtggcctc
     4501 tactacaagc tgcacaatgt taacaggcca ctgaccatga agaaggaagg gatccagacc
     4561 cggaatcgga agatgtccag caaatccaag aaaagcaaga aaggggctga atgtttcgag
     4621 gagctctcca agtgcatgca agagaagtca ccgcccttca gtgcggctgc cctggctgga
     4681 cacatggcac ctgtgggaca cctcccacct tttagtcact ctggacacat cctacccacg
     4741 cccacgccta tccacccttc ctccagtctc tcttttggcc acccccaccc gtccagcatg
     4801 gtgactgcca tgggctagtt cgaagatatc aagcttatcg ataatcaacc tctggattac
     4861 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4921 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4981 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5041 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5101 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5161 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5221 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     5281 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5341 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5401 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa
     5461 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     5521 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     5581 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5641 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     5701 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
     5761 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa
     5821 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     5881 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
     5941 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6001 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6061 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6121 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     6181 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6241 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6301 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6361 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6421 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6481 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6541 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6601 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6661 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6721 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6781 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6841 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     6901 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6961 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7021 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7081 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7141 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7201 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     7261 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     7321 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     7381 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     7441 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     7501 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     7561 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     7621 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     7681 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     7741 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     7801 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     7861 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7921 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     7981 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8041 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8101 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8161 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8221 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8281 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8821 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8881 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9061 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9121 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9181 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9241 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9301 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9361 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9421 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9481 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9541 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9601 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9661 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9721 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9781 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9841 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9901 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9961 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10021 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10081 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10141 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
    10201 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
    10261 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
    10321 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
    10381 ta
//
