LOCUS       sequence-383966-       14094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBolux harboring the promoter region upstream of BT2818.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Modesto JL, Pearce VH, Townsend GE 2nd
  TITLE     Harnessing gut microbes for glycan detection and quantification.
  JOURNAL   Nat Commun. 2023 Jan 17;14(1):275. doi: 10.1038/s41467-022-35626-2.
  PUBMED    36650134
REFERENCE   2  (bases 1 to 14094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-01-17"; volume: "14(1):275. doi"; pages: " 
            10.1038/s41467-022-35626-2"
COMMENT     Sequence Label: P-BT2818
FEATURES             Location/Qualifiers
     source          1..14094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     568..586
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(624..646)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     746..765
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3667..3689
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3681..3698
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3682..3698
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4081..5523
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             7712..8791
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLL"
     primer_bind     complement(9825..9841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9825..9841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9838..9860)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9849..9865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9873..9879,9880..9897,9898..9903))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9918..9939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10056..10073)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10227..10815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10307..10326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10986..11777,11778..11846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11609..11628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11847..11951)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttaatacct cctcgtagca tatgagaact acaggattcc taactcgtat taatatgtat
       61 attattactg ccgcataacg attacaaaat tacacaaaaa aatatatcta acaaaagtag
      121 gaggattttt taacttttta ccatagacat tttattaggg aaacgtaggt tcaactggta
      181 agtacaaata agtagaaatg tttgaacaac actgttttag gaagttgaaa aatcaagttt
      241 ctctctcggt atagcgttta ttttggccaa tatcttcttt agtttggcat ttgagtattt
      301 cccattcgtg ttctatttag ctccttatta tgagtatgta gcaagttgct tatcaaattc
      361 agcctctttg agggtcaaat atggttttgc aaatggtgac tgacaagaca taaacaaggt
      421 cagcaggatt ttttacataa atggacatga atgtatataa cggggtttga gggccaatgg
      481 aacgaaaacg tacgttaagg agataattcg ttgtttatat ttaaatttag ctaagaaacc
      541 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
      601 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
      661 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
      721 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
      781 tccccgaccg atgattttta agtgactgat tttgtgctgt tttgggggta tattaagaat
      841 aaaagaaata gaataagtta agtacttgat acacaatata gggcattttc catattggaa
      901 attctcattt tccaatctag aaaataccga tttcctaata tgatactaaa taggaaaata
      961 atatttccct taatattgtt tttatggaaa ataatagttt actttgtgga gaataatatt
     1021 tcccaaaaac atatcaaaat ggaaaataaa aaagcagtta agttaaccga ttttcaaaag
     1081 aacgaagaaa atccttttat gaaacaagct atagaaggta ttgaaaatca tgttgttaaa
     1141 aagtataaga gtaatagtgg tggcgataag agagctgtag tagctttagc cgacactgaa
     1201 actggagaag tgtttaagac ttcgtttatc cgtcaaatag aagtagatga agaacaattc
     1261 actaaattgt atctttctaa ctttgctgca ttctttgacc tatcacaagc agctattcgg
     1321 gtttttggtt actttatgac ctgcatgaaa cccaaaaatg atttaatcat tttcaataga
     1381 aaaaaatgcc tagaatatac caaatacaaa acagacaaag ccgtttataa aggacttgca
     1441 gaacttgtaa aagctgaaat catagcccga ggaccagccg ataatctttg gtttattaat
     1501 cctctgatag tattcaatgg tgaccgagtg acatttgcta aaacatacgt tcggaaaaag
     1561 actttagctg cccaaaagaa agaagaagca gagaaacgac aattatcact tggctttgat
     1621 gaacagtaac actccattga gtgaagctgc cgtttggtcg ctcccccttt gggggcgggg
     1681 ggggatagat aaagttcctc tatgtaaagt tataatgggg gatgaaaggc aaggttcgct
     1741 aaccttaccc cgattgttta tgcttcccag cattcaatct atccccgttt atgggtttct
     1801 ctttcaccct acaaagataa cctcatgggg gaaaaatgtc caagaatatg gggtaaaaac
     1861 tatcaagtcg gtagaaaata agtatctttg aagtacattt ttagtagagg tactggtgtg
     1921 cctaggcgaa gcagataggc aaaaataaag ccgctggaga tgtcggatgt ccgtcaatta
     1981 actccagcgg cttgtatttt ggtaactctc ccgtatcggt cagctaccgt ttggtcagca
     2041 acaaaggtag tactttatta tcgcaaatcc aaatgttttt ccctttattt gtgcatttac
     2101 aaaacattca ctaacttcgc acctatgcag tatggtgata gcacactagc accctaaaga
     2161 gtgcgtgtgt aaggggacaa gcccccttaa ccccctgtca gccggctgtc accggctgtt
     2221 atcgattgtc acaaaaatgt taacttatgg gagcaacaag tattcatgta caagcagtga
     2281 agccggggag cgagattcac aactttaggg aaaaagagtt ggactatgtt cgtcccgaac
     2341 ttagtcattt gaatgaaagc tgggttggag atagcatttc ccatcggctg gagagtgcaa
     2401 aacaaagata tctcgatacg gttgggcaga agatgcaggc taaagccgca cccatacgag
     2461 agggagtaat agtaatcaaa caagaaacca ctatgcagga actccagcag tttgccacgg
     2521 tctgcaaaga acgtttcggt atcgaagcgt ttcaaatcca tatacacaag gacgaaggat
     2581 acatgaacgc aaagcagtgg acacctaacc ttcatgccca tgtagttttc gattggacgc
     2641 agccgaatgg gaaaagtgtg cgtttatcgc gtgatgacat ggcagaactc caaaccatag
     2701 catctgaaac actgggcatg gaacgtggcg tttcttctga ccgcaaacac ttatcggcta
     2761 tgcagtacaa gaccgaatgt gcgaaagaac agcttcagga actatccaac gatatatcca
     2821 gtgcattaga caaatacaag gacgcacaaa accagcttct tcagcttcag aaggaactac
     2881 gttccattga aacaaagaag aacgtccaaa aactcatttc taaggcttca gagaagtttt
     2941 acggcttgat tggaaaaaca gtcaacgata gggaaaagga tgccttaaaa gccaaagtaa
     3001 agactttaga gggggaaaat gaacaactat ccgatagact gggaaaggct atacttgaaa
     3061 aagagcgaaa cagcacaaag gcattcaaag ccgagaatga caaggagtat tatagacaac
     3121 aaatggacaa cgcaaggaca accagtaacc tattaaggac ggagaaccaa aaactaaaag
     3181 ccgaaaccaa ggagttgaaa aaagaacttg gtaagatgaa agacttgttc aattccgaac
     3241 aactggaggc tttaaggcat catttcccaa acatatcaaa agctatggaa gaggggaaag
     3301 acctattcaa gcaaatcacc agaagcagag gtttcggcat gggtatgtaa cccccccccg
     3361 cccccaaagg gggagcgacc aaacggcagc ctctctcaat ggagtgttac accgttcgga
     3421 ttcagaaaac attttgcagg gttcggaaaa cggttgtgtg cttattctaa aaaaagttta
     3481 tggtgtgtat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     3541 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     3601 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     3661 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacccggg
     3721 gatcctgttc cttacaaagc ctccttttcc attccgtcta taattttgta tcaaggtcta
     3781 cccatttgtt acagatagtg tttctagcta tttgtgcacc taatttggtg aatattgcac
     3841 gtccattttt cacatcaccc ctaattttgc atcatcatta aaagataaat gaagttaacc
     3901 ttattgcaca taagcaaagg atatatttag aaactgaaga gagaataaag agaataaaag
     3961 tcacccccaa cccggttgac ctaactataa actaaatata ttattaacct atcaaattaa
     4021 acagtactag tcactcccgc attttaaaat aaaataaatt atttatttaa ttaaacgaat
     4081 atgactaaaa aaatttcatt cattattaac ggccaggttg aaatctttcc cgaaggtgat
     4141 gatttagtgc aatccattaa ttttggtgat aatagtgttt acctgccaat attgaatgac
     4201 tctcatgtaa aaaacattat tgattgtaat ggaaataacg aattacggtt gcataacatt
     4261 gtcaattttc tctatacggt agggcaaaga tggaaaaatg aagaatactc aagacgcagg
     4321 acatacattc gtgacttaaa aaaatatatg ggatattcag aagaaatggc taagctagag
     4381 gccaattgga tatctatgat tttatgttct aaaggcggcc tttatgatgt tgtagaaaat
     4441 gaacttggtt ctcgccatat catggatgaa tggctacctc aggatgaaag ttatgttcgg
     4501 gcttttccga aaggtaaatc tgtacatctg ttggcaggta atgttccatt atctgggatc
     4561 atgtctatat tacgcgcaat tttaactaag aatcagtgta ttataaaaac atcgtcaacc
     4621 gatcctttta ccgctaatgc attagcgtta agttttattg atgtagaccc taatcatccg
     4681 ataacgcgct ctttatctgt tatatattgg ccccaccaag gtgatacatc actcgcaaaa
     4741 gaaattatgc aacatgcgga tgttattgtc gcttggggag ggccagatgc gattaattgg
     4801 gcggtagagc atgcgccatc ttatgctgat gtgattaaat ttggttctaa aaagagtctt
     4861 tgcattatcg ataatcctgt tgatttgacg tccgcagcga caggtgcggc tcatgatgtt
     4921 tgtttttacg atcagcgagc ttgtttttct gcccaaaaca tatattacat gggaaatcat
     4981 tatgaggaat ttaagttagc gttgatagaa aaacttaatc tatatgcgca tatattaccg
     5041 aatgccaaaa aagattttga tgaaaaggcg gcctattctt tagttcaaaa agaaagcttg
     5101 tttgctggat taaaagtaga ggtggatatt catcaacgtt ggatgattat tgagtcaaat
     5161 gcaggtgtgg aatttaatca accacttggc agatgtgtgt accttcatca cgtcgataat
     5221 attgagcaaa tattgcctta tgttcaaaaa aataagacgc aaaccatatc tatttttcct
     5281 tgggagtcat catttaaata tcgagatgcg ttagcattaa aaggtgcgga aaggattgta
     5341 gaagcaggaa tgaataacat atttcgagtt ggtggatctc atgacggaat gagaccgttg
     5401 caacgattag tgacatatat ttctcatgaa aggccatcta actatacggc taaggatgtt
     5461 gcggttgaaa tagaacagac tcgattcctg gaagaagata agttccttgt atttgtcccg
     5521 taatccgcat ggcaaattgt aaattgtaaa tcgtaaaata gtaatatatt aatggaaaat
     5581 aaatccaaat ataaaaccat cgaccacgtt atttgtgttg aaggaaataa aaaaattcat
     5641 gtttgggaaa cgctgccaga agaaaacagc ccaaagagaa agaatgccat tattattgcg
     5701 tctggttttg cccgcaggat ggatcatttt gctggtctgg cggaatattt atcgcggaat
     5761 ggatttcatg tgatccgcta tgattcgctt caccacgttg gattgagttc agggacaatt
     5821 gatgaattta caatgtctat aggaaagcag agcttgttag cagtggttga ttggttaact
     5881 acacgaaaaa taaataactt cggtatgttg gcttcaagct tatctgcgcg gatagcttat
     5941 gcaagcctat ctgaaatcaa tgcttcgttt ttaatcaccg cagtcggtgt tgttaactta
     6001 agatattctc ttgaaagagc tttagggttt gattatctca gtctacccat taatgaattg
     6061 ccgaataatc tagattttga aggccataaa ttgggtgctg aagtctttgc gagagattgt
     6121 cttgattttg gttgggaaga tttagcttct acaattaata acatgatgta tcttgatata
     6181 ccgtttattg cttttactgc aaataacgat aattgggtca agcaagatga agttatcaca
     6241 ttgttatcaa atattcgtag taatcgatgc aagatatatt ctttgttagg aagttcgcat
     6301 gacttgagtg aaaatttagt ggtcctgcgc aatttttatc aatcggttac gaaagccgct
     6361 atcgcgatgg ataatgatca tctggatatt gatgttgata ttactgaacc gtcatttgaa
     6421 catttaacta ttgcgacagt caatgaacgc cgaatgagaa ttgagattga aaatcaagcg
     6481 atttcgctgt cttaataagg aggaggataa agaatatgac ttcatatgtt gataaacaag
     6541 aaattacagc aagctcagaa attgatgatt tgattttttc gagcgatcca ttagtgtggt
     6601 cttacgacga gcaggaaaaa atcagaaaga aacttgtgct tgatgcattt cgtaatcatt
     6661 ataaacattg tcgagaatat cgtcactact gtcaggcaca caaagtagat gacaatatta
     6721 cggaaattga tgacatacct gtattcccaa catcggtttt taagtttact cgcttattaa
     6781 cttctcagga aaacgagatt gaaagttggt ttaccagtag cggcacgaat ggtttaaaaa
     6841 gtcaggtggc gcgtgacaga ttaagtattg agagactctt aggctctgtg agttatggca
     6901 tgaaatatgt tggtagttgg tttgatcatc aaatagaatt agtcaatttg ggaccagata
     6961 gatttaatgc tcataatatt tggtttaaat atgttatgag tttggtggaa ttgttatatc
     7021 ctacgacatt taccgtaaca gaagaacgaa tagattttgt taaaacattg aatagtcttg
     7081 aacgaataaa aaatcaaggg aaagatcttt gtcttattgg ttcgccatac tttatttatt
     7141 tactctgcca ttatatgaaa gataaaaaaa tctcattttc tggagataaa agcctttata
     7201 tcataaccgg aggcggctgg aaaagttacg aaaaagaatc tctgaaacgt gatgatttca
     7261 atcatctttt atttgatact ttcaatctca gtgatattag tcagatccga gatatattta
     7321 atcaagttga actcaacact tgtttctttg aggatgaaat gcagcgtaaa catgttccgc
     7381 cgtgggtata tgcgcgagcg cttgatcctg aaacgttgaa acctgtacct gatggaacgc
     7441 cggggttgat gagttatatg gatgcgtcag caaccagtta tccagcattt attgttaccg
     7501 atgatgtcgg gataattagc agagaatatg gtaagtatcc cggcgtgctc gttgaaattt
     7561 tacgtcgcgt caatacgagg acgcagaaag ggtgtgcttt aagcttaaac caagcattta
     7621 atagttgata taaatgaagg gtgaagaagg atgaatgaag gtaacactca taaactcgaa
     7681 attcttcatt cttaattttt aattaaaata tatgaaattt ggaaactttt tgcttacata
     7741 ccaacctccc caattttctc aaacagaggt aatgaaacgt ttggttaaat taggtcgcat
     7801 ctctgaggag tgtggttttg ataccgtatg gttactggag catcatttca cggagtttgg
     7861 tttgcttggt aacccttatg tcgctgctgc atatttactt ggcgcgacta aaaaattgaa
     7921 tgtaggaact gccgctattg ttcttcccac agcccatcca gtacgccaac ttgaagatgt
     7981 gaatttattg gatcaaatgt caaaaggacg atttcggttt ggtatttgcc gagggcttta
     8041 caacaaggac tttcgcgtat tcggcacaga tatgaataac agtcgcgcct tagcggaatg
     8101 ctggtacggg ctgataaaga atggcatgac agagggatat atggaagctg ataatgaaca
     8161 tatcaagttc cataaggtaa aagtaaaccc cgcggcgtat agcagaggtg gcgcaccggt
     8221 ttatgtggtg gctgaatcag cttcgacgac tgagtgggct gctcaatttg gcctaccgat
     8281 gatattaagt tggattataa atactaacga aaagaaagca caacttgagc tttataatga
     8341 agtggctcaa gaatatgggc acgatattca taatatcgac cattgcttat catatataac
     8401 atctgtagat catgactcaa ttaaagcgaa agagatttgc cggaaatttc tggggcattg
     8461 gtatgattct tatgtgaatg ctacgactat ttttgatgat tcagaccaaa caagaggtta
     8521 tgatttcaat aaagggcagt ggcgtgactt tgtattaaaa ggacataaag atactaatcg
     8581 ccgtattgat tacagttacg aaatcaatcc cgtgggaacg ccgcaggaat gtattgacat
     8641 aattcaaaaa gacattgatg ctacaggaat atcaaatatt tgttgtggat ttgaagctaa
     8701 tggaacagta gacgaaatta ttgcttccat gaagctcttc cagtctgatg tcatgccatt
     8761 tcttaaagaa aaacaacgtt cgctattata aagaaaggaa taagacaatg gatatgaaat
     8821 ttggattgtt cttccttaac ttcatcaatt caacaactgt tcaagaacaa agtatagttc
     8881 gcatgcagga aataacggag tatgttgata agttgaattt tgaacagatt ttagtgtatg
     8941 aaaatcattt ttcagataat ggtgttgtcg gcgctcctct gactgtttct ggttttctgc
     9001 tcggtttaac agagaaaatt aaaattggtt cattaaatca catcattaca actcatcatc
     9061 ctgtccgcat agcggaggaa gcttgcttat tggatcagtt aagtgaaggg agatttattt
     9121 tagggtttag tgattgcgaa aaaaaagatg aaatgcattt ttttaatcgc ccggttgaat
     9181 atcaacagca actatttgaa gagtgttatg aaatcattaa cgatgcttta acaacaggct
     9241 attgtaatcc agataacgat ttttatagct tccctaaaat atctgtaaat ccccatgctt
     9301 atacgccagg cggacctcgg aaatatgtaa cagcaaccag tcatcatatt gttgagtggg
     9361 cggccaaaaa aggtattcct ctcatcttta agtgggatga ttctaatgat gttagatatg
     9421 aatatgctga aagatataaa gccgttgcgg ataaatatga cgttgaccta tcagagatag
     9481 accatcagtt aatgatatta gttaactata acgaagatag taataaagct aaacaagaga
     9541 cgcgtgcatt tattagtgat tatgttcttg aaatgcaccc taatgaaaat ttcgaaaata
     9601 aacttgaaga aataattgca gaaaacgctg tcggaaatta tacggagtgt ataactgcgg
     9661 ctaagttggc aattgaaaag tgtggtgcga aaagtgtatt gctgtccttt gaaccaatga
     9721 atgatttgat gagccaaaaa aatgtaatca atattgttga tgataatatt aaaaagtacc
     9781 acatgtaagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     9841 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9901 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9961 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    10021 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    10081 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    10141 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10201 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10261 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10321 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10381 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10441 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10501 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10561 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10621 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10681 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10741 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10801 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10861 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10921 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10981 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    11041 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    11101 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    11161 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    11221 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    11281 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11341 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11401 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11461 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11521 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11581 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    11641 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    11701 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11761 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11821 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11881 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11941 ggggttccgc gcacatttcc ccgaaaagtg ccacctgcgt ctattttttt attgccaagt
    12001 tctaatgctt ctatctcctg ccattcatag aggctattat atgccataca tccgataaga
    12061 atggagataa gtatatatcc caactgtatt gccttataga aatttcctga ccgctccatt
    12121 attttgatga cattgatttt tggaacatga ataaaagttt atctttttcg ttcatgcgga
    12181 tattatcaga ataaccgcct tttgttattt gatacccgca tggcttaacc ataaaaatgc
    12241 ctaagccctt agtgtatgaa cttacttctg atgcatagtc tttacttgta tttaatggaa
    12301 ctttcccttt aatatgacac cactcattat tgcaactgat gtcttcaatc tcagacatca
    12361 ttttttgcaa atctgtaata gctttggaac ttgccgcttg gggtatctgc aactcaaaat
    12421 agagcatcgg ttcgagaatg tccacacctg actgttgcaa ggccagcctg aagacataag
    12481 gggtcagctg tctgaaatca gcaggtgtac ttaccgggct ataatactcg gcttgagtaa
    12541 aagttacttt cagatcagtc acttcccatc catgtaaccc ggattggcaa gacatacgaa
    12601 tcccttcaaa aacggcattt tgaaaagaat ggttcagata accataggag atgtcacttt
    12661 cgatttgcaa ccctgtccct aacggtaagg gttcaagagt cagccctatt gtggcccaat
    12721 aagggttggg cggcacttcg atctgaataa tcttattgac cttttttaca ggtcgttctt
    12781 tgtatatagt cttgatctca tcaaaatgga cctttacgga aaatcgttct tccagcaatg
    12841 tctgtatgat ttccttttgg gttaaaccat ataacgagat ttccaattca tcactatatg
    12901 agtttatgga aaaggacaaa gacgggtctt caatccacaa tgtattcaga gcggatatca
    12961 ccttgcttct ctcttcgggc ctgtctggcc ggacggagga tttgagagcg ggatgctgat
    13021 gcgataatcc ttgaatcaaa caaggttcag cacctaaata atttccgatt cgaaaatcat
    13081 ccatatcctc tacaatcgcg atatcattgg cgcccacttc atcaacattt atctctctgc
    13141 cctgattgat agtttttaga tttttaatct tgatgaattt ttccgaatcg ttgattctta
    13201 caacgtctcg aagtctcaga cttccgtcaa ttatttttag aaaacttctt ttatgtcctt
    13261 tggggtcatg ctctatctta taaagataag atgaaagtct gtttgagacc gatgccggag
    13321 gaagtataaa agaagtgatg gcgtccaaca actcattgat accgatattg aacattgctg
    13381 atccatgtag caccggatag actttggctt ttgccacaag agcgattatc gtattccaat
    13441 aatcagccgg tgaaatttcg ctatccgcca aatatcgttc taatatattg tcgtcatggt
    13501 tgcatacaaa ttctttgtat tcttccttta tatatgtttg ggagcaaacc ggataaaccg
    13561 atccatcgac aacattttgc ataaacagga catcttgaga cagatttgct tttatatcca
    13621 gatacaaacg ctccaaattc acaccggctc ggtcaatctt attgataaat ataattgtcg
    13681 ggatttgcag cttctgtaaa gtattgaaca gcaactttgt ctgcgcttgt atgccttcct
    13741 ttgcggataa gatgaggact gctccatcaa gcattttgaa tgtccgctcc acttccgcaa
    13801 taaaatccat gtgtcccgga gtgtcaatga tattgcattt cacaccattc cagataatag
    13861 atgtcgtaga agcccgaaca gtaattcctc tacgtttctc tatatccata gagtccgtta
    13921 tggtgtcacc attatccaca cagccgcact tttccgttgc tccactggca aacagcagat
    13981 tctcggttac ggaagttttt cctgcatcaa tgtgagcaag aattcctaaa tttataatat
    14041 tcatttggat taagcaataa tatactacaa tagatgcatt gtcgaaacgc acct
//
