LOCUS       sequence-383972-        9140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GPR3 and mCherry in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   McNeal AS, Belote RL, Zeng H, Urquijo M, Barker K, Torres R, Curtin 
            M, Shain AH, Andtbacka RH, Holmen S, Lum DH, McCalmont TH, 
            VanBrocklin MW, Grossman D, Wei ML, Lang UE, Judson-Torres RL
  TITLE     BRAF(V600E) induces reversible mitotic arrest in human melanocytes 
            via microrna-mediated suppression of AURKB.
  JOURNAL   Elife. 2021 Nov 23;10. pii: 70385. doi: 10.7554/eLife.70385.
  PUBMED    34812139
REFERENCE   2  (bases 1 to 9140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-11-23"; volume: "10. pii: 70385. doi"; pages: " 
            10.7554/eLife.70385"
COMMENT     Sequence Label: pLVX-Phi3-GPR3-PGK-mCherry
FEATURES             Location/Qualifiers
     source          1..9140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    204..319
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        377..680
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        681..884
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     835..855
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     881..905
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2036..2535
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2071..2094)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2468..2487
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2554..2563
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2560..3270
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2705..2723)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2973..2992
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3284..3872
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3337..3357)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3755..3766)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4079..4712
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4841..4857)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4841..4857)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4854..4876)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4865..4881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4889..4895,4896..4913,4914..4919))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4934..4955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5072..5089)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5243..5831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5323..5342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6002..6793,6794..6862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6625..6644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6863..6967)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7015..7149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7052..7071)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7106..7125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7317..7950
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7997..8122
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8619..8852
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9037..9081
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
       61 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
      121 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
      181 gaacggatct cgacggtatc gcctttaaaa gaaaaggggg gattgggggg tacagtgcag
      241 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
      301 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttatcgat
      361 aagcttggga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      421 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      481 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      541 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      601 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      661 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      721 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      781 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      841 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      901 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctact
      961 agaggatcgc tagcgctgaa gcttagatct cgagaattcg cggccgctcc ggacgcgtat
     1021 gatgtggggg gctgggtctc cactggcctg gctgtctgct ggtagcggaa acgtgaacgt
     1081 tagttccgtg ggacccgctg aggggccaac gggcccagca gcgcctctcc ctagtcccaa
     1141 agcttgggac gtcgtgttgt gcatttcagg caccctggtt tcctgtgaga acgcccttgt
     1201 ggtggccatt atcgttggca caccagcgtt tagggcccct atgttcctgc ttgtgggctc
     1261 cctggccgtt gcggatttgc tggctgggct cggtctcgtg ttgcactttg ctgcagtatt
     1321 ttgtatcgga agcgccgaga tgtccctcgt gctcgtgggc gtgcttgcta tggctttcac
     1381 agcttcaatc ggcagcttgc tcgccattac tgtagaccgc tacctgtccc tgtacaacgc
     1441 attgacctac tactcagaaa ccacagtcac aagaacctac gtaatgctgg cactggtttg
     1501 gggtggcgca ctgggccttg gacttcttcc cgtgttggcc tggaactgtc ttgatgggct
     1561 cactacctgt ggagttgtct accctttgag caagaatcat ctggtagtgc tcgccatagc
     1621 cttttttatg gtgttcggga ttatgcttca gctgtatgcc caaatttgca ggatcgtttg
     1681 taggcacgca cagcagattg ctttgcagag gcacctgctt ccagctagcc actatgtggc
     1741 gactcggaaa ggcatcgcaa ccttggccgt cgtgttgggg gcttttgccg catgctggct
     1801 gccctttacg gtgtattgcc tgctgggaga tgcccatagt ccccctcttt acacttatct
     1861 gacgctcctg cctgcgacgt ataactccat gattaatcct atcatctacg ccttccggaa
     1921 tcaggatgta cagaaggtgc tctgggcagt ctgttgctgc tgtagctctt caaaaatccc
     1981 cttcaggtcc cggagcccct ccgatgtcta gttaattaat ctagataatt ctaccgggta
     2041 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     2101 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     2161 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     2221 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     2281 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     2341 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     2401 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     2461 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     2521 tctccgggcc tttcgacctg cctcgaggcc cgggccacca tggtgagcaa gggcgaggag
     2581 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     2641 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     2701 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     2761 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     2821 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     2881 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     2941 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     3001 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     3061 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     3121 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     3181 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     3241 accggcggca tggacgagct gtacaagtaa ccgggtctgg aacaatcaac ctctggatta
     3301 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3361 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3421 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3481 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3541 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3601 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3661 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     3721 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3781 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3841 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta
     3901 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     3961 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     4021 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg
     4081 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     4141 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     4201 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     4261 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     4321 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     4381 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     4441 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     4501 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     4561 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     4621 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     4681 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     4741 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg
     4801 ttttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4861 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4921 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4981 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5041 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5101 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5161 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5221 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5281 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5341 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5401 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5461 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5521 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5581 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5641 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5701 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5761 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5821 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5881 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5941 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6001 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6061 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6121 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6181 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6241 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6301 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6361 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6421 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6481 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6541 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6601 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6661 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6721 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6781 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6841 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6901 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6961 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg
     7021 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7081 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7141 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     7201 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
     7261 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     7321 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     7381 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     7441 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     7501 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     7561 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     7621 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     7681 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     7741 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     7801 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7861 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7921 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     7981 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     8041 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     8101 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     8161 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     8221 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     8281 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     8341 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     8401 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     8461 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     8521 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     8581 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     8641 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     8701 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     8761 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     8821 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     8881 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     8941 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9001 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9061 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9121 aaattattca taatgatagt
//
