LOCUS       sequence-383995-       14058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad host range IncQ promoter probe using lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14058)
  AUTHORS   Spaink HP, Okker RJ, Wijffelman CA, Pees E, Lugtenberg BJ
  TITLE     Promoters in the nodulation region of the Rhizobium leguminosarum 
            Sym plasmid pRL1JI.
  JOURNAL   Plant Mol Biol. 1987 Jan;9(1):27-39. doi: 10.1007/BF00017984.
  PUBMED    24276795
REFERENCE   2  (bases 1 to 14058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Mol Biol"; date: "1987-01"; volume: "9"; issue: "1"; pages: 
            "27-39"
COMMENT     Sequence Label: pMP190
FEATURES             Location/Qualifiers
     source          1..14058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1937..1993
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2125..2144)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2236..5283
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(2238..2260)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(5801..6652)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6639..7478)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(7989..8960)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            10199..10286
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      10628..11022
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
ORIGIN
        1 tcagaatggc gtgttccggt gacttctgtt ttcgtgtgct gcatccggaa gtgtcaggcc
       61 cgccggatat gaaaaaggcc tccggagagt accgtgactt tattttcggc acaaatacag
      121 gggtcgatgg ataaatacgg cgatagtttc ctgacggatg atccgtatgt accggcggaa
      181 gacaagctgc aaacctgtca gatggagatt gatttaatgg cggatgtgct gagagcaccg
      241 ccccgtgaat ccgcagaact gatccgctat gtgtttgcgg atgattggcc ggaataaata
      301 aagccgggct taatacagat taagcccgta tagggtatta ttactgaata ccaaacagct
      361 tacggaggac ggaatgttac ccattgagac aaccagactg ccttctgatt attaatattt
      421 ttcactatta atcagaagga ataaccatga attttacccg gattgacctg aatacctgga
      481 atcgcaggga acactttgcc ctttatcgtc agcagattaa atgcggattc agcctgacca
      541 ccaaactcga tattaccgct ttgcgtaccg cactggcgga gacaggttat aagttttatc
      601 cgctgatgat ttacctgatc tcccgggctg ttaatcagtt tccggagttc cggatggcac
      661 tgaaagacaa tgaacttatt tactgggacc agtcagaccc ggtctttact gtctttcata
      721 aagaaaccga aacattctct gcactgtcct gccgttattt tccggatctc agtgagttta
      781 tggcaggtta taatgcggta acggcagaat atcagcatga taccagattg tttccgcagg
      841 gaaatttacc ggagaatcac ctgaatatat catcattacc gtgggtgagt tttgacggat
      901 ttaacctgaa catcaccgga aatgataatt attttgcccc ggtttttacg atggcaaagt
      961 ttcagcagga aggtgaccgc gtattattac ctgtttctgt acaggttcat catgcagtct
     1021 gtgatggctt tcatgcagca cggtttatta atacacttca gctgatgtgt gataacatac
     1081 tgaaataaat taattaattc tgtatttaag ccaccgtatc cggcaggaat ggtggctttt
     1141 ttttatattt taaccgtaat ctgtaatttc gtttcagact ggttcaggat cactgtacga
     1201 taatgccccc gcagtttggt aataccctta ataaaaaaga aacagcaaag actgacagca
     1261 ataataataa agtaagcagt aacaataata ttaacaacac cagatgcagt tataataata
     1321 gtatttaaga caccagaaag actgctgcga cagtcatttt gaacaacacc aaaatgccgt
     1381 aaaggcagta gtaacaacac cagtgaaaac atcacgatag catagtgata tgcctgagtg
     1441 tgtgtaatta aacaataaat aaaccgccat atataacaga agatagtatt ctgaatggca
     1501 tgcttttctg ttcagtataa acatatcatc ccggttggta taaggatgat atataataag
     1561 ttaagctgaa cacatattta ttttggtttt attttacaaa taaagtaaga cgatccgtta
     1621 agtcaaagcg gggtatattt attataccct gcttttttat ttgtccgccg ggcgcggata
     1681 atggatcaga ttatgcagtg tcacaatggc cttaccggga ttggcgtaag cgtgcgggat
     1741 atccgcatgg aagcgcaggg attccccggc agaaacggtg tgccactcat cccccagccg
     1801 cagttgtaat gcgccttcca gtacaatgac atgttctctg gttctgaaat ccatccctgt
     1861 cggtgttgct tatgcagtct ggtcgggact cggcgtcgtc ataattacag ccattgcctg
     1921 gttgcttcat gggcaaaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac
     1981 cgagctcgaa ttcatcgata tctagatctc gagctcgcga aagcttssay gmswktttca
     2041 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     2101 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     2161 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     2221 ttttatccgg atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     2281 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     2341 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt
     2401 ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact
     2461 gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg
     2521 acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac
     2581 tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt
     2641 gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag
     2701 gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc
     2761 ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg
     2821 cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc
     2881 gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa
     2941 gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt
     3001 gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt
     3061 ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc
     3121 gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt
     3181 gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg
     3241 ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat
     3301 ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac
     3361 tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac
     3421 cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg
     3481 aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg
     3541 gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc
     3601 cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg
     3661 gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac
     3721 gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg
     3781 ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt
     3841 aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag
     3901 ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac
     3961 ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg
     4021 aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag
     4081 cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg
     4141 ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg
     4201 gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct
     4261 gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg
     4321 aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg
     4381 gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc
     4441 gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc
     4501 tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag
     4561 ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct
     4621 aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg
     4681 cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag
     4741 catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa
     4801 atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc
     4861 ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa
     4921 gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca
     4981 gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa
     5041 ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt
     5101 caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg
     5161 ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta
     5221 tcggcggaat tacagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa
     5281 taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc cattatgtac
     5341 tatttatcta gatcagacgt cgtggatgtc ggaccggccg ccagccatcc ggacgcgagg
     5401 cctgtatcgc cggccgatga gatcagacgt attgcgccgc tcttagacgc cctgtccgat
     5461 cagatgcacc gtgtttcaat cgacagcttc caaccggaaa cccagcgcta tgcgctcaag
     5521 cgcggcgtgg gctacctgaa cgatatccaa ggatttcctg accctgcgct ctatcccgat
     5581 attgctgagg cggactgcag gagcagaaga gcatacatct ggaagcaaag ccaggaaagc
     5641 ggcctatgga gctgtgcggc agcgctcagt aggcaatttt tcaaaatatt gttaagcctt
     5701 ttctgagcat ggtatttttc atggtattac caattagcag gaaaataagc cattgaatat
     5761 aaaagataaa aatgtcttgt ttacaataga gtgggggggg tcagcctgcc gccttgggcc
     5821 gggtgatgtc gtacttgccc gccgcgaact cggttaccgt ccagcccagc gcgaccagct
     5881 ccggcaacgc ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca ctggcctctg
     5941 acggccagac atagccgcac aaggtatcta tggaagcctt gccggttttg ccggggtcga
     6001 tccagccaca cagccgctgg tgcagcaggc gggcggtttc gctgtccagc gcccgcacct
     6061 cgtccatgct gatgcgcaca tgctggccgc cacccatgac ggcctgcgcg atcaaggggt
     6121 tcagggccac gtacaggcgc ccgtccgcct cgtcgctggc gtactccgac agcagccgaa
     6181 acccctgccg cttgcggcca ttctgggcga tgatggatac cttccaaagg cgctcgatgc
     6241 agtcctgtat gtgcttgagc gccccaccac tatcgacctc tgccccgatt tcctttgcca
     6301 gcgcccgata gctacctttg accaccatgg catcagcggt gacggcctcc cacttgggtt
     6361 ccaggaacag ccggagctgc cgtccgcctt cggtcttggg ttccgggcca agcactaggc
     6421 cattaggccc agccatggcc accagccctt gcaggatgcg cagatcatca gcgcccagcg
     6481 gctccgggcc gctgaactcg atccgcttgc cgtcgccgta gtcatacgtc acgtccagct
     6541 tgctgcgctt gcgctcgccc cgcttgaggg cacggaacag gccgggggcc agacagtgcg
     6601 ccgggtcgtg ccggacgtgg ctgaggctgt gcttgttctt aggcttcacc acggggcacc
     6661 cccttgctct tgcgctgcct ctccagcacg gcgggcttga gcaccccgcc gtcatgccgc
     6721 ctgaaccacc gatcagcgaa cggtgcgcca tagttggcct tgctcacacc gaagcggacg
     6781 aagaaccggc gctggtcgtc gtccacaccc cattcctcgg cctcggcgct ggtcatgctc
     6841 gacaggtagg actgccagcg gatgttatcg accagtaccg agctgccccg gctggcctgc
     6901 tgctggtcgc ctgcgcccat catggccgcg cccttgctgg catggtgcag gaacacgata
     6961 gagcacccgg tatcggcggc gatggcctcc atgcgaccga tgacctgggc catggggccg
     7021 ctggcgtttt cttcctcgat gtggaaccgg cgcagcgtgt ccagcaccat caggcggcgg
     7081 ccctcggcgg cgcgcttgag gccgtcgaac cactccgggg ccatgatgtt gggcaggctg
     7141 ccgatcagcg gctggatcag caggccgtca gccacggctt gccgttcctc ggcgctgagg
     7201 tgcgccccaa gggcgtgcag gcggtgatga atggcggtgg gcgggtcttc ggcgggcagg
     7261 tagatcaccg ggccggtggg cagttcgccc acctccagca gatccggccc gcctgcaatc
     7321 tgtgcggcca gttgcagggc cagcatggat ttaccggcac caccgggcga caccagcgcc
     7381 ccgaccgtac cggccaccat gttgggcaaa acgtagtcca gcggtggcgg cgctgctgcg
     7441 aacgcctcca gaatattgat aggcttatgg gtagccattg attgcctcct ttgcaggcag
     7501 ttggtggtta ggcgctggcg gggtcactac ccccgccctg cgccgctctg agttcttcca
     7561 ggcactcgcg cagcgcctcg tattcgtcgt cggtcagcca gaacttgcgc tgacgcatcc
     7621 ctttggcctt catgcgctcg gcatatcgcg cttggcgtac agcgtcaggg ctggccagca
     7681 ggtcgccggt ctgcttgtcc ttttggtctt tcatatcagt caccgagaaa cttgccgggg
     7741 ccgaaaggct tgtcttcgcg gaacaaggac aaggtgcagc cgtcaaggtt aaggctggcc
     7801 atatcagcga ctgaaaagcg gccagcctcg gccttgtttg acgtataacc aaagccaccg
     7861 ggcaaccaat agcccttgtc acttttgatc aggtagaccg accctgaagc gcttttttcg
     7921 tattccataa aacccccttc tgtgcgtgag tactcatagt ataacaggcg tgagtaccaa
     7981 cgcaagcact acatgctgaa atctggcccg cccctgtcca tgcctcgctg gcggggtgcc
     8041 ggtgcccgtg ccagctcggc ccgcgcaagc tggacgctgg gcagacccat gaccttgctg
     8101 acggtgcgct cgatgtaatc cgcttcgtgg ccgggcttgc gctctgccag cgctgggctg
     8161 gcctcggcca tggccttgcc gatttcctcg gcactgcggc cccggctggc cagcttctgc
     8221 gcggcgataa agtcgcactt gctgaggtca tcaccgaagc gcttgaccag cccggccatc
     8281 tcgctgcggt actcgtccag cgccgtgcgc cggtggcggc taagctgccg ctcgggcagt
     8341 tcgaggctgg ccagcctgcg ggccttctcc tgctgccgct gggcctgctc gatctgctgg
     8401 ccagcctgct gcaccagcgc cgggccagcg gtggcggtct tgcccttgga ttcacgcagc
     8461 agcacccacg gctgataacc ggcgcgggtg gtgtgcttgt ccttgcggtt ggtgaagccc
     8521 gccaagcggc catagtggcg gctgtcggcg ctggccgggt cggcgtcgta ctcgctggcc
     8581 agcgtccggg caatctgccc ccgaagttca ccgcctgcgg cgtcggccac cttgacccat
     8641 gcctgatagt tcttcgggct ggtttccact accagggcag gctcccggcc ctcggctttc
     8701 atgtcatcca ggtcaaactc gctgaggtcg tccaccagca ccagaccatg ccgctcctgc
     8761 tcggcgggcc tgatatacac gtcattgccc tgggcattca tccgcttgag ccatggcgtg
     8821 ttctggagca cttcggcggc tgaccattcc cggttcatca tctggccggt ggtggcgtcc
     8881 ctgacgccga tatcgaagcg ctcacagccc atggccttga gctgtcggcc tatggcctgc
     8941 aaagtcctgt cgttcttcat cgggccacca agcgcagcca gatcgagccg tcctcggttg
     9001 tcagtggcgt caggtcgagc aagagcaacg atgcgatcag cagcaccacc gtaggcatca
     9061 tggaagccag catcacggtt agccatagct tccagtgcca cccccgcgac gcgctccggg
     9121 cgctctgcgc ggcgctgctc acctcggcgg ctacctcccg caactctttg gccagctcca
     9181 cccatgccgc ccctgtctgg cgctgggctt tcagccactc cgccgcctgc gcctcgctgg
     9241 cctgctgggt ctggctcatg acctgccggg cttcgtcggc cagtgtcgcc atgctctggg
     9301 ccagcggttc gatctgctcc gctaactcgt tgatgcctct ggatttcttc actctgtcga
     9361 ttgcgttcat ggtctattgc ctcccggtat tcctgtaagt cgatgatctg ggcgttggcg
     9421 gtgtcgatgt tcagggccac gtctgcccgg tcggtgcgga tgccccggcc ttccatctcc
     9481 accacgttcg gccccaggtg aacaccgggc aggcgctcga tgccctgcgc ctcaagtgtt
     9541 ctgtggtcaa tgcgggcgtc gtggccagcc cgctctaatg cccggttggc atggtcggcc
     9601 catgcctcgc gggtctgctc aagccatgcc ttgggcttga gcgcttcggt cttctgtgcc
     9661 ccgcccttct ccggggtctt gccgttgtac cgcttgaacc actgagcggc gggccgctcg
     9721 atgccgtcat tgatccgctc ggagatcatc aggtggcagt gcgggttctc gccgccaccg
     9781 gcatggatgg ccagcgtata cggcaggcgc tcggcaccgg tcaggtgctg ggcgaactcg
     9841 gacgccagcg ccttctgctg gtcgagggtc agctcgaccg gcagggcaaa ttcgacctcc
     9901 ttgaacagcc gcccattggc gcgttcatac aggtcggcag catcccagta gtcggcgggc
     9961 cgctcgacga actccggcat gtgcccggat tcggcgtgca agacttcatc catgtcgcgg
    10021 gcatacttgc cttcgcgctg gatgtagtcg gccttggccc tggccgattg gccgcccgac
    10081 ctgctgccgg ttttcgccgt aaggtgataa atcgccatgc tgcctcgctg ttgcttttgc
    10141 ttttcggctc catgcaatgg ccctcggaga gcgcaccgcc cgaagggtgg ccgttaggcc
    10201 agtttctcga agagaaaccg gtaagtgcgc cctcccctac aaagtagggt cgggattgcc
    10261 gccgctgtgc ctccatgata gcctacgaga cagcacatta acaatggggt gtcaagatgg
    10321 ttaaggggag caacaaggcg gcggatcggc tggccaagct cgaagaacaa cgagcgcgaa
    10381 tcaatgccga aattcagcgg gtgcgggcaa gggaacagca gcaagagcgc aagaacgaaa
    10441 caaggcgcaa ggtgctggtg ggggccatga ttttggccaa ggtgaacagc agcgagtggc
    10501 cggaggatcg gctcatggcg gcaatggatg cgtaccttga acgcgaccac gaccgcgcct
    10561 tgttcggtct gccgccacgc cagaaggatg agccgggctg aatgatcgac cgagacaggc
    10621 cctgcggggc tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc
    10681 taaaagcgct ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc
    10741 ctttccccct gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga atagaccagc
    10801 tatccggcct ctggccgggc atattgggca agggcagcag cgccccacaa gggcgctgat
    10861 aaccgcgcct agtggattat tcttagataa tcatggatgg atttttccaa caccccgcca
    10921 gcccccgccc ctgctgggtt tgcaggtttg ggggcgtgac agttattgca ggggttcgtg
    10981 acagttattg caggggggcg tgacagttat tgcaggggtt cgtgacagtt agtacgggag
    11041 tgacgggcac tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa
    11101 ctttccgcta agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct
    11161 catgtggcgg ccaggacggc cagccgggat cgggatactg gtcgttacca gagccaccga
    11221 cccgagcaaa cccttctcta tcagatcgtt gacgagtatt acccggcatt cgctgcgctt
    11281 atggcagagc agggaaagga attgccgggc tatgtgcaac gggaatttga agaatttctc
    11341 caatgcgggc ggctggagca tggctttcta cgggttcgct gcgagtcttg ccacgccgag
    11401 cacctggtcg ctttcagctg taagcgtcgc ggtttctgcc cgagctgtgg ggcgcggcgg
    11461 atggccgaaa gtgccgcctt gctggttgat gaagtactgc ctgaacaacc catgcgtcag
    11521 tgggtgttga gcttcccgtt tcagctgcgt ttcctgtttg gggtcgtttg cgggaagggg
    11581 cggaatccta cgctaaggct ttggccagcg atattctccg gtgagattga tgtgttccca
    11641 ggggatagga gaagtcgctt gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg
    11701 ccgcgatagc tagatcgcgt tgctcctctt ctccatccgc gttccaagct gcggaaaggc
    11761 acccataagc gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc gcacgagaga
    11821 atgcgtccgc catctgtgca atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg
    11881 gatcgtagaa catattggcg gcgccaaagc ccacttcacc gaccagaccg acgggatcta
    11941 tcaccagcca gccgcgactg gagaacatga tgttttcatg atgcagatcg ccatgtagcc
    12001 cacgcagttc cgaggcattg ctcatcattt gatcggctat aatcgccgcg tggacgtagt
    12061 cagtttgaca acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat
    12121 cccggatcgg gagaagggca gaaggcaggg gttcctcaga tgcggcatac agcttcgcca
    12181 ttagttccgc tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga
    12241 gcattcgctc cccggcatat tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga
    12301 ctgctcccct cccattgcgc cataccagat agtcggcccc gcgcagttca tcagcaatgt
    12361 cttctatagg tttcaatccc ttgacgattg caggagtccc gtctggcaat gaaactttcc
    12421 aaacgaggct ggaaaaggtg tccgcaatga gaacaggttg cgaaacgtgc caatgagcag
    12481 gaaaaacagg cggcatgaac atcaacccca agtcagaggg tccaatcgca gatagaaggc
    12541 aaggcgttcg cggtcggggg cttcgatccc caatacattg aataggacag cgaaggcgcg
    12601 ctctgcttca tctggcgctg cccagttctc ttcggcgtta gcaatcatga gtgccaaatc
    12661 ggcatagcga tctgctgttc cgagccgccc aaggtcgatc agacccgtgc attgaagagt
    12721 tttagggtcc accatgaagt tcggcatgca gggatcacca tggcaaacaa ccatatcggt
    12781 gcgctcttgg tcgagccgca ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg
    12841 cggcgtactc ttgtcctcgt ccggtaagaa gtcgggattg acggcattgc gggacaccac
    12901 atcaacggcg cgtccgaaca ttcgcgacag cctgcgctca aacggacatt gatcaaccga
    12961 taggctgtga acagcgccaa gttgctgccc cattgacggc cacgctttga gcaaatccgc
    13021 tccagacaga tcagccgccg gtactcccgg aattgccgtt atcaccaagc atgcaccctc
    13081 ctgttcctcc tgccagttga tgacctcggg gcaagccaca cctcgacctt tgagccaaat
    13141 gaggcggtca cgctctccag cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa
    13201 ggcatgcccg tcaccacgtc gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa
    13261 ccagtcagaa tgcgattcac caaaaaaaat attagttcga ttcaatggag gttccttcag
    13321 ttttctgatg aagcgcgaat atagagaaat atcccgaatg tgcagttaac gaattcttgc
    13381 ggtttctttc agcgccgcca ataccgccag cccgtcgcgc aaggggcgcg gctcgtgtgt
    13441 gcggatgaag tcagctccac ctgcggcggc ggcaagctct gcagagcgcc accgcgccat
    13501 gacctggatg caacgcctca agcgagtctt caatattgat attgaagtct gcgaacactg
    13561 cggcggtcac gtcaaagtga ttgccagcat cgaagatccg aaggtcattg agcagattct
    13621 caagcatctg aaacagaaaa cagccaaggc gaatgccgcc aagcagcgtg agctgccacc
    13681 agaacgagcg ccgccactga ctcccagcct gttcgatcca tcacagagtc gtctctttga
    13741 ctgacgaccc caaatccaac actgctcaac actgccaact tttaaacggg gcggtggggc
    13801 agtttgtatc tctcgagcta tcaggctaga gattttaccg ccaaatcgaa ccttattaga
    13861 gcggtttagg ctggaccggc agttaaaatt ggggcttgag cggtaaacga gtgagggaat
    13921 ttcaggtaag atacttcgga tgaggagcaa aaaggtggtt tatacttcct atacccaaaa
    13981 aggtggttta tacttcctat accccggtta tccggtggtt ttctgtttgc ggaataaaac
    14041 agattagtga cagttttc
//
