LOCUS       sequence-384001-        5501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5501)
  TITLE     SGC Toronto 2022-Q1 to Q2
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PATL2_G288-Y543_BV_his
FEATURES             Location/Qualifiers
     source          1..5501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  142..366
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(433..966)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1136..1141,1142..1158,1159..1164))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1516..1607
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1532..1549
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1666..1683
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1696..1716
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2622..2756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2659..2678)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2713..2732
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2785..2950
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3134..3588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3221..3240)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3430..3451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3614..3718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3719..3787,3788..4579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3937..3956)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4750..5338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5239..5258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(5492..5501,1..8)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
       61 tttcacaccg catagaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      121 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      181 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      241 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      301 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ccaagggcat ggtaaagact
      361 atattcgcgg cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc
      421 ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg
      481 tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta
      541 gatcacataa gcaccaagcg cgttggcctc atgcttgagc agattgatga gcgcggtggc
      601 aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg
      661 cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc
      721 gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc
      781 cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc
      841 agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact
      901 tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca
      961 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     1021 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     1081 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt
     1141 acgaaccgaa caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg
     1201 tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga
     1261 gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg
     1321 caaggtgctg tgcacggatc tgccctggct tcaggagatc ggaagacctc ggccgtcgcg
     1381 gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg
     1441 cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc tacgtatact
     1501 ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca aataaataag
     1561 tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt
     1621 cataccgtcc caccatcggg cgcggatctc ggtccgaaaa ccatgcatca tcatcatcat
     1681 cacagcagcg gcagagaaaa cttgtatttc cagggcggga ctcaagagca ggatattgaa
     1741 gccgccagtt cgcagcggtt aagagtgctc tatagaattg aaaagatgtt tctccagctt
     1801 ttagagattg aggagggctg gaaataccgc cctcccccac cgtgttttag cgaacaacag
     1861 tccaaccaag tcgaaaaact tttccaaacc ctaaaaacac aggagcagaa taacctggag
     1921 gaagcggctg acggtttctt acaggttcta agtgtcagga aaggtaaggc gctcgtagcg
     1981 cggctattgc cgtttcttcc acaagaccaa gcagtcacaa tactcttagc tatcactcac
     2041 cacctcccac tattggtgcg gagggacgtt gcagaccaag ctctgcaaat gttgtttaag
     2101 ccccttggta aatgcatttc ccacctcacg ctccatgagc ttctgcaagg actccaagga
     2161 ttgacacttt tgccccctgg atcctctgaa cgacccgtga cagtcgtttt gcagaatcag
     2221 tttggaatta gtctactgta cgcactttta tcacatgggg agcaattggt gagcttgcac
     2281 tcttcactgg aagagcccaa cagcgaccac acggcatgga ccgatatggt agtactgatc
     2341 gcttgggaga tagcgcaaat gcctacagct tcgcttgctg agcctctggc atttcctagt
     2401 aacctgttgc ccctattctg tcaccatgtc gataaacaat tagtccagca gctagaggca
     2461 cgaatggagt ttgcttggat ctattgatga cgaagcttgt cgagaagtac tagaggatca
     2521 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     2581 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     2641 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2701 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat
     2761 cactgatatc gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg
     2821 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     2881 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     2941 tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact
     3001 ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct
     3061 gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg
     3121 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3181 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3241 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3301 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3361 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3421 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     3481 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3541 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     3601 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3661 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3721 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3781 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3841 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3901 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3961 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4021 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4081 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4141 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4201 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4261 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4321 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4381 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4441 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4501 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4561 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4621 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4681 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4741 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4801 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4861 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4921 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4981 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5041 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5101 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5161 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5221 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5281 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5341 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5401 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     5461 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca g
//
