LOCUS       sequence-384030-        5129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage Lambda.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_Lambda
FEATURES             Location/Qualifiers
     source          1..5129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(59..78)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     219..241
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(397..1275)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1302..1586
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1509..1528
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3179..3206
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3225..3316
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3317..3385,3386..4177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3535..3554)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4348..4936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4837..4856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5090..5107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    join(5122..5129,1..133)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
       61 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
      121 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      181 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
      241 gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa
      301 gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta
      361 ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc
      421 attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt
      481 aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
      541 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca
      601 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa
      661 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
      721 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
      781 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
      841 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
      901 cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc
      961 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
     1021 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
     1081 aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
     1141 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
     1201 cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt
     1261 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
     1321 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt
     1381 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
     1441 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
     1501 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
     1561 atcgcaactc tctactgttt ctccataccc gttttttggg ctaacaggag gaattaacca
     1621 tgggttatca cggcggtggc agcggatttg gagggcagtt gcggtcgtgg aacccaccga
     1681 gtgaaagtgt ggatgcagcc ctgttgccca actttacccg tggcaatgcc cgcgcagacg
     1741 atctggtacg caataacggc tatgccgcca acgccatcca gctgcatcag gatcatatcg
     1801 tcgggtcttt tttccggctc agtcatcgcc caagctggcg ctatctgggc atcggggagg
     1861 aagaagcccg tgccttttcc cgcgaggttg aagcggcatg gaaagagttt gccgaggatg
     1921 actgctgctg cattgacgtt gagcgaaaac gcacgtttac catgatgatt cgggaaggtg
     1981 tggccatgca cgcctttaac ggtgaactgt tcgttcaggc cacctgggat accagttcgt
     2041 cgcggctttt ccggacacag ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca
     2101 ataccggcga cagccggaac tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc
     2161 tgggatatta cgtcagcgag gacgggtatc ctggctggat gccgcagaaa tggacatgga
     2221 taccccgtga gttacccggc gggcgcgcct cgttcattca cgtttttgaa cccgtggagg
     2281 acgggcagac tcgcggtgca aatgtgtttt acagcgtgat ggagcagatg aagatgctcg
     2341 acacgctgca gaacacgcag ctgcagagcg ccattgtgaa ggcgatgtat gccgccacca
     2401 ttgagagtga gctggatacg cagtcagcga tggattttat tctgggcgcg aacagtcagg
     2461 agcagcggga aaggctgacc ggctggattg gtgaaattgc cgcgtattac gccgcagcgc
     2521 cggtccggct gggaggcgca aaagtaccgc acctgatgcc gggtgactca ctgaacctgc
     2581 agacggctca ggatacggat aacggctact ccgtgtttga gcagtcactg ctgcggtata
     2641 tcgctgccgg gctgggtgtc tcgtatgagc agctttcccg gaattacgcc cagatgagct
     2701 actccacggc acgggccagt gcgaacgagt cgtgggcgta ctttatgggg cggcgaaaat
     2761 tcgtcgcatc ccgtcaggcg agccagatgt ttctgtgctg gctggaagag gccatcgttc
     2821 gccgcgtggt gacgttacct tcaaaagcgc gcttcagttt tcaggaagcc cgcagtgcct
     2881 gggggaactg cgactggata ggctccggtc gtatggccat cgatggtctg aaagaagttc
     2941 aggaagcggt gatgctgata gaagccggac tgagtaccta cgagaaagag tgcgcaaaac
     3001 gcggtgacga ctatcaggaa atttttgccc agcaggtccg tgaaacgatg gagcgccgtg
     3061 cagccggtct taaaccgccc gcctgggcgg ctgcagcatt tgaatccggg ctgcgacaat
     3121 caacagagga ggagaagagt gacagcagag ctgcgtaatg ccaggcatca aattaagcag
     3181 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta
     3241 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3301 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3361 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3421 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3481 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3541 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3601 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3661 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3721 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3781 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3841 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3901 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3961 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4021 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4081 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4141 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4201 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4261 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     4501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5101 gagcgaggaa gcggaagagc gcctgatgc
//
