LOCUS       sequence-384034-        8848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs1 from Escherichia coli NCTC9036.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 8848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: EcAvs1
FEATURES             Location/Qualifiers
     source          1..8848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(238..897)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     812..831
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(898..1000)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1392..1409)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1526..2071)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1606..1625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      join(8819..8848,1..18)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gtcttgaggg gttttttgct gaaacctcag gcatttgaga agcacacggt cacactgctt
       61 ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc
      121 cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat
      181 cacttattca ggcgtagcac caggcgttta agggcaccaa taactgcctt aaaaaaatta
      241 cgccccgccc tgccactcat cgcaatactg ttgtaattca tttaacattc tgccgacatg
      301 gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
      361 ttgcgtataa tatttgccca tagtgaaaac gggggcgaag aagttgtcca tattggccac
      421 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
      481 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
      541 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
      601 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
      661 gtctttcatc gccatacgga actctggatg agcattcatc aggcgggcaa gaatgtgaat
      721 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
      781 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
      841 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
      901 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
      961 ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa
     1021 gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg
     1081 atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg ccaacttact
     1141 gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc
     1201 cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc
     1261 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
     1321 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
     1381 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
     1441 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
     1501 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
     1561 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
     1621 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     1681 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
     1741 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
     1801 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
     1861 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
     1921 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
     1981 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
     2041 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
     2101 tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg
     2161 atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg
     2221 ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca
     2281 ttaattgcgt tgcgcagaaa tttcgcgaca gagatcctta acggtgcgtc gagcttcgac
     2341 ggaattcaga ataatgatgg tctggtgttc ggtgaatcgt gctttgcgca tggcgatctc
     2401 ctatcagaac aaaaccagta tgccggatga tctctaaaag tgaatggacc gatatgcagg
     2461 gatgcttaca gtgggtcttc gacctttata agcatagtaa agaatagaat atgccaatgt
     2521 acgataatct gtgcactcta ttacctgcgc aaaaaagtac accagaattg tttgtctggt
     2581 ttggcaaatt gagatcatta ggcggcatag cgaatgactt taaatgaaaa gcccgattca
     2641 tcaataaaga ttgttaaaac aaaaaccttg cccccagcag agggcgagcg ccgggcaatg
     2701 cgtggctata tgggccaata tgaaagagcc ggtgcagcca tttatgctga attagagcgt
     2761 gggcaattgg agtggatagg cgtagcggac cgcagtgcgg gtatcgttga tgatttagta
     2821 cttggattta atggccttat cgttgggcac cagttcaaaa cgtcccgttt ccctggtaca
     2881 tttacagtac agacactctt agtagggtct gatggtctgc ttaagccatt agtttgcgcc
     2941 tggcaaaatc tttgtagtgc taacccaacg tctcaggtag aaattcgttt agttgtcaac
     3001 gattatccat cagttaacga cgctcccgga atggaagctc cagctcatag cgctgccttc
     3061 cttgatgagt ttgaacatta tcccaaacgc acgcttgagg aatggcgcta cagtaactgg
     3121 ggccgtttag tcgaaatatt atttcaacat tcctgcctag gtgacgatga tttcgagaga
     3181 ttttttcatg cgttgcgcat aattcatggt tctgcagcag attttataca attccataaa
     3241 ctcagtgcag aacaagcgag actggcgtct gatatagcaa aaatattacc tcgactggtc
     3301 tccgataaac gagataggga tcgatggtcc tgtgaagaac tattatatga actagggtgg
     3361 aaagatccca ccaaaacacg ccacttacat cgttttccca tcggtgctca cgtccaacgc
     3421 aaccgcgata cggaactaca acttctccag acgatacgca acacaatcca gggctatgtg
     3481 gcattgattg ggcctccagg ttcggggaaa tcgaccttgc tacagacaac cctagctacc
     3541 gagtataaca ctcgggtcgt gcgctatctg gctttcatac cgggcgctgc gcaaggtgta
     3601 gggcgcgggg aagctgatga tttcttcgaa gacatttctg cccagttacg cagcagcggg
     3661 ctgcctggac ttcgccttcg agacagcagc caatttgaaa ggcgcgaaca attcggtgaa
     3721 ctgctcaaac aagctggcga gcgttatcaa cgtgatacag taagaaccat cattattgtt
     3781 gatgggctgg atcatatccc ccgcgaagaa ctaccagccc attcgctgtt aggggaattg
     3841 ccgctgcctg cagccatccc tttgggcgtg acatttatac ttggcaccca gcgactggaa
     3901 ctcaggcatc tcaaacccgc agtacaggaa caggctgggc atccggatcg tctcgtaaca
     3961 atgcatccac ttgagagagt ggcggtcgcc aggatggcag acgttttagg tcttgattca
     4021 accatttcgc gtgtaaaact ttatgaactt agccgcggtc atccgctggc ggccaattat
     4081 ctcattaagg cactgttatc ggctgatgaa caggacatat catgcatcct cgccggaggg
     4141 atggaattta atggcgatat tgaatcagtt tacgcatctg cctggagaga aatcgcaaac
     4201 gaccctgatg ttatgcatgt actgggtttc attgcccgtg tcgaagctcc gatgccgctg
     4261 aaattgctgg caacaatcgt agatgctcag gcgatagagc gtaccttaaa gaccgtccgg
     4321 catttactca aggaaacctc aaaggggtgg actgtattcc ataacagctt ccgtctattt
     4381 gtgctctcca aaccaaagat aacactgggc agtatagatg aaacctattc acaacatatt
     4441 tatcgtgaat tagctaaact atctcgtcat gcaccagaac attcattaca gtcctggcta
     4501 acactgcgct atctcgcccg gtcaggagag cgtgatgaac ttctggcact cgcaactcca
     4561 gcatattttc gacaccagtt tgcacatgga cgttcctgtt cagagattga tgcggacatt
     4621 cacttggctc tgattgctgc gcgttccacg tatgatggtg taattgccac acggttatta
     4681 ctttgccgtg atgagatatc cagacgaact caagcactgg agtatgccaa tgaacttccg
     4741 cgcgcgatgt taaaagttgg cgatattgat gcggcgatct ctttcgtcca ggactttccc
     4801 aatgcgggct atgaagttgt tgaccttctt ttggaacagg gtgattttga ccgcgcgaaa
     4861 gaactgtttg agcaccttga gccattatct caattgcata cccccagatt cgagcactat
     4921 ggggattcgc ataatctaca agaattcaaa aaatgggcaa aacgagttgt tcacttccgc
     4981 gacgctgagc aaattaagca ggcaatagac tatttgaccg ttgaggggtt taaacacgcc
     5041 acaagtgtat caaccgatga aaatatttcc tctattcgcg aacagttaaa gtggacagtg
     5101 gtcgaggcaa ttgttaactg gcaatcagac gttaatattc aggatacctg caatcagtat
     5161 ggcattcatg tgcaagagat accggttttg atgactcagg ctggatttat tgctagagac
     5221 agaggaaata acaccttagc atcggaatta tttaagactg ccatggcatt gtctgatttt
     5281 aatgatgttt ctaatggggg gcgaagatcg attgcattat tttatgccac atcaggctgc
     5341 accgatctgg cttcaaaatt attcgaaaac ctttttgcgc ctgcaatttc gatgggagac
     5401 aatgaattag aatcaacaaa agcactgacg cttgcagcca tggaacatgc gcaactttgc
     5461 gttttgctcg gcaaatcctt gcccgacgta gtcacctcaa cacacgctat cttacgaccg
     5521 ctgcagacac atgcttcaga aacgggacgc ttgttggggc tgtccataat aaatgcctca
     5581 tgtattcctt ctggaaatat taaaatggtc tgtcgcatgg tgatgagata tgtaatgcaa
     5641 ctcaatagct attctggaaa cgatacctat caggctcaat tggcattgac agctacatca
     5701 ccactgattt gtacattaat taaaatttct gcgctgtgtg gtaaggttga atattattca
     5761 gtaataaatg aaattgataa tgcaatgcct gctttaatat taaaaggcaa tacactactc
     5821 cggcgtgaaa tagcattggc aatgtatcag gctgacggtg accgtgaaag ggcggccgcc
     5881 agatttgagc ctatggtaaa cgagttggta gaaaatacac ctagcgagca actcgagact
     5941 ctgtcagttc tggcaaacag ctttgctgca attggcgatg ttgaccgggc actaaactta
     6001 cttgcttcga tacatgacca ctgtttaggc tacgctctgg cagcgcgtaa ggacccttta
     6061 tactctgttt ggaaagacat attgattttg gccaatgcgg cagacccaga acaccgtgct
     6121 caacgaatag gtcagttgat acgacaggtt gatggtatga aggaaaccga gggagcatct
     6181 gccgcatatc gtttgacaga agtgttaatc aatgaagcaa tgcgtatgaa tgcgcacagt
     6241 ggttataccg tggcacagaa actcagcaac tgggggctga ttccatggcc aaatcaggta
     6301 aatgaactgg taattggtat gctagatcgc cgtcctgaaa tggtgtttct ctgtacacaa
     6361 atttggtgcg ggctatgcct tccattctac attgaaccct attatcgtga ccctacacat
     6421 gtaggcaatt atattgacgt tgctgcaaat gcagcggggc cttcatcaat tgccaaactg
     6481 gtatcaattc tattaccggc aatccaggtt catagtcgag ctcacgagcg actcacgcta
     6541 ataaatcgcc tgagcaaggc ggcattaaga cacggttata ccgataacca acttgataat
     6601 gccattactc gatggacttc agaggccccc gaagcccgcc gctcctacac gccacaaacg
     6661 tacgacgaag cttcaaccct tgacgaactt caacaggcat ttgaatcaaa tgattccgaa
     6721 cctgagtatc atgcgcctta tcgtttttgt gagcttgcag agtccgccgc attagacaag
     6781 gtggtgaaaa tgtatgagtg ctggcattgc ctgcagtcgg atgcacgttg tcgttttttg
     6841 gttgcagagc ggctagttaa tgcgggggac acgacgttag ccagaaaatt agttgatgat
     6901 tacgatacca gtagtgaccg ggagatgtca tggagccaat ggttaggagg aaatcgattc
     6961 cgtctcttcc acgcgcgtaa gctactcgat ggagcagcaa ttcatcatga agcatatgaa
     7021 gacttcatca gttcaattgt ggctgggaaa gagagcacca tgtcgttgct aacagatatg
     7081 gcagacattc ttcctgtgat ctgtgagtcg ccagactggc ccgccgtctg gtctatcctg
     7141 gcagagcaga tgtctttcac tcgcgaacac cgtattggtg aacttttcga atttggaaat
     7201 gaaaatatga ccgacgaaga gttacttgcg gaattgctcc atttttcatt acgattgcct
     7261 atcaccgaag ctcgacgaca cgcagagaaa actgcactaa ttctggcggt acattcaaca
     7321 ggagggcaaa tcgtatttga gaacaccata acacgactcc tgaacggcac ccttgatgaa
     7381 ccattccagg cattgcaaat tttgcttttg ctaaaacaga accactttgc tgctaaattt
     7441 ggtgatttag tctctggcct tacgaatcat cgtgatgtag ctgttgctga agctgcgtgc
     7501 ttgttagcac aatattggca gctacctgta tcgattgatt ttcatccgtt gccgttgacc
     7561 tatcgattgg cactcgacgg agaccctgat catgaaaatg ctctgttaga tcctgtgagt
     7621 ggggcaatgc gtattgaagt cgacttagga tggacacaaa tgcttcgtcc cgttgcacgg
     7681 agacttgcag agtttgctga ttgtgacgaa atgaacatac gccagcgtgc cgcaacgttt
     7741 attcagcaat ggggagggct ggcagccttt ggccctggag caacaaaaaa aatcgaatct
     7801 cagttacgca cactctcaat gcaaatcacc tatcttaagc cccatgctta cattggcata
     7861 ctggcacttc gtcatgtcgc tggagagctg agcttggcag gcttgctctc gccaagggat
     7921 aaaccatcgc tactggaaca aatggatgca gtacttccgc caactcctcg ccctgaaatg
     7981 caaatccggc caactggcat taggcgaccg cttaaagtca aggatgcccc gtggagtgaa
     8041 gctgaagaaa tgtggacaaa tttggttgac gaggatgtta aaccctggat aggtcgtgcc
     8101 gacgaattcg taatagccga ggtttcacaa ttcaaaatgc atgatacccg gcgtgctgaa
     8161 tatcaggtct atcgtattag cgcacctcaa attcatattt ctgatgccaa attcatggca
     8221 tggtatcaaa gtttgcccgc tgtcgtttgg ctgggaaaaa tgatcccact tgacgaagac
     8281 ctcgcaccga caatagtcag gcgtgtagta agctccatcg ggacaatgtc ttcgccggga
     8341 tatgccattg cattatgtcc taatatccag atgcatctgg gatggcatga atgctgcgag
     8401 atgcctaata tttataccga ccagaactca acaatcgtag caagattagt gaactggcga
     8461 gacgccgggc cagtggatat tgatgatgat tatatatggg gggaaggttg ctatctgacg
     8521 ctttccaatg caggcctgat acaagtcaag actctgttcg gcgaattcac cgtgcgtaat
     8581 ttcgcaagca gggctgttcg gcaattgcga caaggcgaag cgcaaatgat aaagacagct
     8641 cagaatcagt tcccgatact gtagcgagac gatttcacaa cacggttcga ttacctgact
     8701 tctccaacca tggtctgaag aagtcaggga gtgtagatca tgccggcatt ctgtttctga
     8761 atggcgcagg atttcgggtc agggtcacca caacaggctt gtccttttct cgcaataact
     8821 agcataaccc cttggggcct ctaaacgg
//
