LOCUS       sequence-384035-        8627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Codon optimized KpAvs4 with C-terminal His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: pCDF-KpAvs4
FEATURES             Location/Qualifiers
     source          1..8627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(449..1531)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1493..1512
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1532..1609)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1655..1674
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1655..1673
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1674..1698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             6851..6868
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6900..6918)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6914..6961
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(7132..7923)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(7924..8015)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctacactgg aaggacagat ttggttgctg tgctctgcga aagccagtta ccacggttaa
       61 gcagttcccc aactgactta accttcgatc aaaccacctc cccaggtggt tttttcgaag
      121 gttaagtcag ttggggaact gcttaacctt cgatcaaacc acctccccag gtggtttttt
      181 cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      241 tttctactga accgctctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag
      301 tcagccccat acgatataag ttgtaattct catgttagtc atgccccgcg cccaccggaa
      361 ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt
      421 gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      481 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      541 ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct
      601 ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct
      661 gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca
      721 ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca
      781 gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt
      841 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct
      901 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag
      961 aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca
     1021 cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag
     1081 agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct
     1141 ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca
     1201 ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac
     1261 ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca
     1321 gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc
     1381 ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag
     1441 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact
     1501 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg
     1561 ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga
     1621 cgctctccct tatgcgactc ctgcattagg aaattaatac gactcactat aggggaattg
     1681 tgagcggata acaattcccc tgtagaaata attttgttta actttaataa ggagatatac
     1741 catgaacgaa aacctgaaga aacaaagcaa cccgttcagc accggtggcg gtggcgtgaa
     1801 ctttgagacc cgtgttcaag cggcgtttgc gctgagcatc ctgaccggta gcagcgtgcc
     1861 gtgcctgagc caaagcatgc gtgcgaagca gattcgtttt caaaacaaat acgacggcag
     1921 caacaccgac gatttcgtga tcgttgcgga agatagcgac ggtcaagagg gcaagctgtt
     1981 tgcgcagatt aaacacgaaa tcaccctgag cgagagcccg gatagcgtgt tcgcggaagt
     2041 gatcgcgagc gcgtggcagg attttaagag cgacaacttc aaaatggatc acgacgtgat
     2101 tgttctgatc accggtccgc tgcaaaagac cgacattcac aacaccctgc cgatcctgga
     2161 atgggcgaaa tttgcgctga gcgcggagga tttcatgaag aaagttcaga ccaagggctt
     2221 tagcagcgaa gcgaaactga agaaaatcga gaacttccgt ctgcacctgc gtcacgcgag
     2281 caacattagc gaaatcgacg ataccttcct gtggagcttt ctgaagagct tccacctgat
     2341 tagctacgat ctggacagca gcttcagcgt ggttgcgagc ctgctgtgca gccagatcca
     2401 aaacaacagc gatgaggcgc cgccgctggt gctgagcaaa atcattacca ccattcagaa
     2461 ctttaaccaa aacgcgggta ttctgagcga ggaaaacatc ccgcacgatc tgaagaccct
     2521 gttcaacaag gttcacaaga aaaacatcaa ggacgacatc cgtaaactgc aggaacgtgg
     2581 tacctacatt tataccggca tcagcaaccg tatttgcggc attcacatca accgtaacga
     2641 acaactggcg gagattgaaa agaaatgcca ggagagccgt ttcgtgtttg ttaccggtat
     2701 ccgtggtgtg ggcaagagcg gcattgttaa agatttcatc agcaccaagt acaaagacgt
     2761 gccgttcttt tatctgcgta ccgaggatct ggacaagagc cacctgaacg acgtttttac
     2821 cgcgctgggt atgagctgca gcctggcgga aattgcgggc gagttcagcc tgctgaagga
     2881 aaaaatcctg atcattgaga gcgtggaaaa ggttctggag ctgacctacc aaaacgcgtt
     2941 tattgatctg ctgcacttca tcaaaaacca gaacggttgg accgtgattg cgaccggccg
     3001 tgactatgcg acccagcaac tgatcttcaa ctacctgcag ccgagcggta ttgattattg
     3061 caacgttgaa atcaaggcgc tggagtacca gcaactgagc gaaatctgcc acaaaattcc
     3121 ggagctggaa aagctgattg agaacaaacg tctgaccgat ctgctgaagg tgccgttctt
     3181 tatcgacgtg gcggttcgtg cgctgaaaaa cggtgcgcaa tttcacaccg agggcaccga
     3241 acgtgatttc cgtgaaatca tttggagcag cgttatcgcg aaagagaccg accgtcgtgg
     3301 tggcatgccg gcgaagcgta aacgtacctt tattgacatc gcgaaacagc gtgcgaagaa
     3361 aatggtggtt ggtatcctgg acgatgcgtt cgatccggaa atcattgcga agctggagga
     3421 agacaacctg attcaacgtc acgaacgtct gaacctgatc agcccgagcc acgatgtgct
     3481 ggaggactgg gcgctggata tttttatcga caacgagtac gtggaaaact tcctggttcc
     3541 gaacaaattc tttgaacaga ttggcgacga gccgagcatc aaccgtgcgt atcgtctgtg
     3601 gctgagccgt aacatcaagg agaacaagga aatcaacgat ttcatctaca aggttctgac
     3661 cgacgcgagc attgcgagct attggaaaga tgagaccatt accgcgatcc tgcaaagcga
     3721 cgcggtgaac atctttctga agatgttccg tacccagctg ctgagcaaca aaaacctgct
     3781 gctgattcgt gttctgttca tcctgcgtat gacctgccag caaccgaaga ccctgtacaa
     3841 ccaagagctg gaaaaagagg agcaggaaat tagcaaaacc ctgtacctgc aaccgtatgg
     3901 tatctgctgg gacgcgctga ttaacttcat ctttgaaaac cagaacgagc tggtgaacag
     3961 caccagcatc cacatcattg agatgctgaa cagctgggtt agcattctga acatcagcga
     4021 taactatccg gacattagcc gtaaagtggg cctgctggcg atctggctgc tggaaccgct
     4081 gaaagatagc tacgcgcgtg aggacgaata taagcgtgag aaagttatta gcctgctgct
     4141 gaagctgagc ccgaccatcg tggatgaatt tgacctgctg atggataaag aggttttcat
     4201 cccggacacc agccaccgtc gtctgcacta cgtggagtat ctgattaacc agagcctgat
     4261 cggtctgaac gttccgattc tgtgcaagta ccgtccggat ttcatcgaac gtctggcgcg
     4321 tcacgagtgg ctgcaagaca acattgaaga cgatgacagc atcttttaca aacaaaccac
     4381 cggtgtggat cagtatttcg gcctgaaccg tgagagcgac ttctttccgg cgagcggtgc
     4441 gaagggcccg tttaaatacc tgctgcactt ccacccgcgt aaggcgctgg attttatcat
     4501 tgacctgtgc aacaaaaccg cgaagaaata tagcaccagc gaatttgcga cccgtagcag
     4561 cggtttcatg agcgatgaaa ccctggtgaa gaacgttgag ctgaccctga acgacggcac
     4621 cgtggttacc cagttcagca gcccgcacct gtggaaaggt taccgtggcc acagcaccct
     4681 gccgtatatt ctgcaatgcg cgctgatggc gctggagaac tggctgatcg atagcctgga
     4741 cagcctggag aagaacgaca ccattgagtg gctgtttaac tacatcctgc tgagcagcaa
     4801 cagcgttatg ccgaccagcg tgctggcgag cgttgcgacc ggtttcccgg acaagattag
     4861 caaagcggcg tttccgatcc tgcgttgccc ggagttctac aacctggaca tccaacgtct
     4921 ggtgaacgaa cgtggtggca gcgagcagaa ctggtttagc aacatgttcc acaacgatat
     4981 tatgaacgac acctatgttg aggagcgtcg tattgcggcg ctgcgtccgt ggcgtaagga
     5041 gaccctggaa agcctgctga cccgtctgca gctgagcggc aagaacaaag cggataccct
     5101 gaaaattgtg gacagcctga tcgcgctgag cctggaaagc aacgacgaga acctgcgttt
     5161 tctggttcac cgtgcggata cccgtacctg gagcttcacc gcggacaagg aaaacagccg
     5221 tatcattgcg cagagcaacc cggagctgcc ggaagatctg cagcaaattc acgacgagca
     5281 cagcgcgacc cacacccaaa acagcctgat caacaagctg tttctgtgga gccgtaaaag
     5341 cttcgaggaa aacatcaaca acacctactt taacagccac accgaagcgc tgaccgcggc
     5401 gaagtacctg ttcgataaat ttcagaacaa cgaggtggac aacccggtga tggttattgg
     5461 taccatcacc aagaccgcgg cggtttgcac ccgtgacctg ttcgaaaacc tgagcgagga
     5521 cgagaagaac tggtgcaaga aaatcattat cgagtgcatt atcatgaacg cggactgcat
     5581 caacggcctg agcctgcacg ataaaaccga cagctatggt agcggtgcgt gcgcgttcgt
     5641 gctgccgaag ctgttccgtt atgttaaaaa cgacgaggaa ctgaacgaac tgaagttcac
     5701 cctggcgatc gcgctgaccc acgaaaacat tgacatcggc gagtacgcgg cgaaaggcgt
     5761 gcgtgagttt ctgtggacca cccacccgac cctggcgttc cagtgcattt atggtgcgac
     5821 catgttcgcg cgttttaaga aagaaaacat caacagccaa cgtttttacc tgtatagcga
     5881 taccgagctg gcgcaggcga aggcgcactg gagctacctg atcaccagct tccgtgagga
     5941 attctttaac accaccccgc cgctgattga gaacatcagc ctgaaaaccc acagcagctg
     6001 gcacatccac ctgccgattc tgatgatccc gttctgcgtg gacaacccgc tgctgattaa
     6061 cctgtacaag aaaatcgttc acttcacctt tgatgaggaa tataagcgtc acaaaagcaa
     6121 ccacgatgac gaaaccatcc aacacgacat tcgtaagcag atcaccaact gctttgcgga
     6181 gcacttcatc cacacccgta agaccggttt cgtgccgttt aaaaccctgc tgctggaagg
     6241 ttgcagcacc gcgccgcgta ttgtttatat gaccaagctg ctgtttcaca tcgcgatgga
     6301 gaaagcggaa gacctggaga gcctgtggga attctggctg atgctggcgc cggaggcgca
     6361 caagattgcg ctgaaagatg ttaacgagca gtacagcggt atccaaagcg acctgaacca
     6421 gctgctgcgt ggtatgctgt atgcggatac ccgttggcag ggccacgaga acgaagcgcg
     6481 tgacatcgcg tacggtagcg tgcacattct ggactttgcg aagatcagcg gcaacaacag
     6541 ccacgttttt atggcgctga gcagcctgat ttatcacttc caccacatct tctttgacga
     6601 aggcattcac atcctggcgg agaaactgat catgaacagc aagctgattg ataaacaaat
     6661 caacaccgcg ttctacctgg aaatggcgat tggtcgttat ctgcagattg agaaccgtgg
     6721 catcctgagc cgtaaaatgt acaacagctg catgaccctg ctgagcggta ttgtggaaac
     6781 cggcagcgcg cgtgcgtact atctgcgtga gaacctgatc cgtagccgtc gtgttttcac
     6841 cggcggaagc catcaccatc atcaccacag cagcggctaa ttaacctagg ctgctgccac
     6901 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     6961 gctgaaacct caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc
     7021 ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg
     7081 gtcatcgtgg ccggatcttg cggcccctcg gcttgaacga attgttagac attatttgcc
     7141 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg
     7201 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg
     7261 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat
     7321 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc
     7381 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag
     7441 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct
     7501 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa
     7561 gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg
     7621 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat
     7681 ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt
     7741 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag
     7801 gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg
     7861 ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct
     7921 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7981 atacatattt gaatgtattt agaaaaataa acaaatagct agctcactcg gtcgctacgc
     8041 tccgggcgtg agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt
     8101 ggataagcag gggactaaca tgtgaggcaa aacagcaggg ccgcgccggt ggcgtttttc
     8161 cataggctcc gccctcctgc cagagttcac ataaacagac gcttttccgg tgcatctgtg
     8221 ggagccgtga ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag
     8281 atccccaccg tttccggcgg gtcgctccct cttgcgctct cctgttccga ccctgccgtt
     8341 taccggatac ctgttccgcc tttctccctt acgggaagtg tggcgctttc tcatagctca
     8401 cacactggta tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac
     8461 tccccgttca gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg
     8521 aaaagcacgg taaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt
     8581 tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca aagtccg
//
