LOCUS       sequence-384048-        9332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ATP measurement, mitochondria outer membrane, lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Yaginuma H,  Okada Y
  TITLE     Live cell imaging of metabolic heterogeneity by quantitative 
            fluorescent ATP indicator protein, QUEEN-37C
  JOURNAL   bioRxiv 2021.10.08.463131
REFERENCE   2  (bases 1 to 9332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.10.08.463131"
COMMENT     Sequence Label: pLBS-CAG-TOMM-QUE37C
FEATURES             Location/Qualifiers
     source          1..9332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..236
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             237..417
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    464..589
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1082..1315
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1500..1544
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1811..1927
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2006..2309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2311..2586
                     /label=chicken beta-actin promoter
     intron          2587..3603
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3527..3549
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3611..3630
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     3659..3675
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3660..3676
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      3697..3706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4162..4171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4528..4779
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     4716..4737
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4843..4864)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5104..5123)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    5350..5938
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5403..5423)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5821..5832)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6029..6262
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6334..6415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6371..6390)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6425..6444
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(6493..6511)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6532..6548)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6532..6548)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6545..6567)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6556..6572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6580..6586,6587..6604,6605..6610))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6625..6646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6763..6780)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6934..7522)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7014..7033)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7693..8484,8485..8553))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8316..8335
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8554..8658)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8684..9139)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8821..8842)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9033..9052
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9266..9288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9280..9297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9281..9297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9304..9323
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9304..9322
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taccacgcgt gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
       61 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
      121 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
      181 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
      241 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      301 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      361 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
      421 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
      481 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
      541 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
      601 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
      661 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
      721 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
      781 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
      841 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
      901 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
      961 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1021 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1081 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1141 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1201 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1261 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1321 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1381 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1441 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1501 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1561 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1621 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     1681 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     1741 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     1801 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     1861 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca
     1921 aaattttggg cccttccaac cttccaaccg gtacctagta atcaattacg gggtcattag
     1981 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     2041 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2101 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2161 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2221 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2281 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
     2341 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
     2401 gtgcagcgat gggggcgggg gggggggggg ggcgcgccag gcggggcggg gcggggcgag
     2461 gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
     2521 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
     2581 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
     2641 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
     2701 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
     2761 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
     2821 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
     2881 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     2941 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     3001 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
     3061 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     3121 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     3181 ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc
     3241 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     3301 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
     3361 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     3421 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     3481 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     3541 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     3601 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa aggattcctc
     3661 gaggtcgacg gtatcgataa gcttgatatc gaattcgcca ccatggtggg tcggaacagc
     3721 gccatcgccg ccggtgtatg cggggccctt ttcattgggt actgcatcta cttcgaccgc
     3781 aaaagacgaa gtgaccccaa cttcaagaac aggcttcgag aacgaagaaa gaaacagaag
     3841 cttgccaagg agagagctgg gctttccaag ttacctgacc ttaaagatgc tgaagctgtt
     3901 cagaagttct tccttgaaga aatacagctt ggtgaagagt tactagctca aggtgaatat
     3961 gagaagggcg tagaccatct gacaaatgca attgctgtgt gtggacagcc acagcagtta
     4021 ctgcaggtct tacagcaaac tcttccacca ccagtgttcc agatgcttct gactaagctc
     4081 ccaacaatta gtcagagaat tgtaagtgct cagagcttgg ctgaagatga tgtggaaggc
     4141 ggtagcgggg atccaccggt cgccaccatg gtgaaaactg tgaaagtgaa tataacaacc
     4201 cctgatgggc cagtctacga cgctgatatc gagatggtgt ccgtgcgggc cgagagtggt
     4261 gatctcggca tcctccccgg tcacattccc acaaaggccc cactgaagat cggagctgtg
     4321 cggctgaaga aggacggcca aacagaggat gtcgcagtct caggcggcac tgttgaagtg
     4381 cggcctgacc acgttaccat taatgctcaa gccgctgaac gggcggagga cattgacgtc
     4441 ctccgcgcca aagcggcgaa agagcgggcg gagcgccgcc tgcaaagcca gacccgttac
     4501 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     4561 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     4621 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     4681 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     4741 gccgccggga tcactctcgg catggacgag ctgtacaagg gtggcaccgg agggagcatg
     4801 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     4861 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     4921 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4981 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     5041 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     5101 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     5161 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     5221 ctggagtaca acctgggtga catcgacttc aaacgggccg aactggcgtt aaaacgcgcc
     5281 atgaaccgtt tgagcgttgc ggaagataag tgagcggccg cgactctaga tcactagtct
     5341 cgactcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5401 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5461 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5521 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5581 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5641 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5701 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     5761 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5821 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5881 ccgcatcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     5941 ggaattaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6001 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa
     6061 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6121 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6181 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6241 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6301 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     6361 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6421 agttgtggtt tgtccaaact catcaatgta tctagagcgg ccgccaccgc ggtggagctc
     6481 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
     6541 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6601 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6661 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6721 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6781 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6841 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6901 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6961 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7021 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7081 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7141 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7201 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7261 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7321 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7381 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7441 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7501 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7561 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7621 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7681 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7741 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7801 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7861 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7921 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7981 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8041 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8101 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8161 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8221 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8281 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8341 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8401 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8461 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8521 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8581 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8641 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     8701 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     8761 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     8821 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     8881 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     8941 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     9001 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     9061 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     9121 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     9181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     9241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     9301 ttgtaatacg actcactata gggcgaattg gg
//
