LOCUS       sequence-384059-        8736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9-mediated generic protein tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Seibert M, Kurrle N, Stolp V, Nurnberger H, Tzschentke S, Borner L, 
            Wempe F, Serve H, Schnutgen F
  TITLE     Selection-free endogenous tagging of cell lines by bicistronic 
            co-expression of the surface antigen NGFR.
  JOURNAL   MethodsX. 2022 Nov 18;9:101929. doi: 10.1016/j.mex.2022.101929. 
            eCollection 2022.
  PUBMED    36444289
REFERENCE   2  (bases 1 to 8736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MethodsX.";
            date: "2022-11-18"; volume: "9:101929. doi"; pages: " 
            10.1016/j.mex.2022.101929. eCollection 2022"
COMMENT     Sequence Label: pGTag NGFR EGFP nLAP -2
FEATURES             Location/Qualifiers
     source          1..8736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    314..335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(350..355,356..373,374..380)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    388..404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     393..415
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     412..428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     412..428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     447..467
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        449..467
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(559..582)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(598..618)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(631..675)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(715..738)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     1157..1176
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(1483..1539)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_RNA        complement(2480..2555)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(2634..2653)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(2863..2882)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2864..2882)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2892..2909)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2892..2908)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2901..2923)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3049..3504)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3186..3207)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3398..3417
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3531..3635
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3636..3704,3705..4496)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3854..3873)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4667..5255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5156..5175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(5507..5526)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(5548..5877)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5562..5697
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5862..5882
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     6018..6040
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     LTR             6143..6568
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     CDS             complement(7115..8134)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             complement(7115..8134)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    8173..8635
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8333..8352)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     8562..8579
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
       61 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      121 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
      181 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      241 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      301 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      361 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      421 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
      481 gtaccgggcc ccccctcgag acatcaggta tgtcgggaac ctctccagga acgaggccct
      541 gggcaggtcg tcggtcagct tgtcgtcgtc gtccttatag tcccctccgc ctccgcctcc
      601 ctgaaagtac aggttctcgc ctcctcctcc gctgtccatg tgctgccgct cgaatttagc
      661 agcagctgtt tctttgaact tggcggcgct gccagagggg ccagagctgc tcagggggcc
      721 ctggaacagc acttccaggc tgctgctgcc gctggggccg ctgctcttgt acagctcgtc
      781 catgcccagg gtgattccgg cggctgtcac aaattctttc agcaccatgt ggtcccgctt
      841 ctcgttgggg tccttgctga gagcgctctg ggtgctcagg tagtggttgt cgggcagcag
      901 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggccagct gcacgctgcc
      961 gtcctcgatg ttgtgccgga tcttgaagtt gaccttgatg ccgttcttct gcttgtcggc
     1021 cataatgtac acgttgtggc tgttgtagtt gtattccagc ttgtggccca ggatgttgcc
     1081 gtcctctttg aagtcgatgc ccttcagctc gatcctgttc accagtgtgt cgccctcgaa
     1141 cttcacctcg gccctggtct tgtagttgcc gtcgtccttg aaaaagatgg tgcgctcctg
     1201 gacgtagccc tcgggcatgg cgctcttgaa gaaatcgtgc tgcttcatgt ggtcggggta
     1261 tctgctgaag cactgcacgc cgtaggtcag ggtggtcacc agggtgggcc agggcacggg
     1321 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg cgtcgccttc
     1381 gccctcgccg gacacgctga acttgtggcc gttcacgtcg ccgtccagct ccaccaggat
     1441 gggcaccacg ccggtgaaca gttcctcgcc cttggacacc atgggcccgg ggttttcttc
     1501 aacatctcct gcttgcttta acagagagaa gttcgtggct ccgcggagga tccccctgtt
     1561 ccacctcttg aaggctatgt aggccacaag gcccacaacc acagcagcca ggatggagca
     1621 atagacaggg atgaggttgt cggtggtgcc tcgggtcacc acgggctggg agctgcccat
     1681 cactgtggtc accacacctg ccaccgtgct ggctatgagg tcttgttctg gaggtgcctc
     1741 aggctcctgg gtgctggggg ctgtgctgtc cgagccctct gggggtgtgg accgtgtaat
     1801 ccaacggcca gggatctcct cgcactcggc gtcggcccag cgtgtgcact cgcggagctg
     1861 gcgctcggtg tcctcgcaca cggtgcaggg caggcacggg tccacgtggt tggcctcgtc
     1921 ggaatacgtg ccgtcggggc actcctcgca cacggtgttc tgcttgtcct ggcaggagaa
     1981 cacgaggccc gagcccgcct cgcacacgcg gcacgcctcg cagcgcccag tcgtctcatc
     2041 ctggtagtag ccgtaggcgc agcggcacac ggcgtcgtcg gcctccacgc acggcgccga
     2101 catgctctgg agccccacgc actcggtgca cggcttgcac ggctcggtcg cgctcaccac
     2161 gtcggagaac gtcacgctgt ccaggcaggg ctcacacacg gtctggttgg ctccacaagg
     2221 ctgggccaca ccctcgccca ggttgcaggc tttgcagcac tcaccgctgt gtgtgtacag
     2281 gcctgtgggg catgcctcct tggcacctcc aagggacacc cccagaagca gcaacagcag
     2341 caggcgcggc ccgtccatgg cgcggccggt ggcacctgcc cccatggtgg tacttaggta
     2401 tgtcgggaac ctctccaggg agtagtcgac ggtatcgata agctagatgt cctgaaatgt
     2461 aagatctgaa ttcaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta
     2521 gccttatttt aacttgctat ttctagctct aaaacggaga ggttcccgac ataccggtgt
     2581 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
     2641 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
     2701 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa
     2761 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaatagtc
     2821 ggatcttagg gataacaggg taatgcgggt agctccaatt cgccctatag tgagtcgtat
     2881 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2941 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     3001 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg
     3061 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3121 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3181 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3241 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     3301 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3361 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3421 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3481 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3541 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3601 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3661 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3721 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3781 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3841 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     3901 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3961 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4021 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4081 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4141 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4201 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4261 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4321 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4381 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4441 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4501 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4561 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4621 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4681 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4741 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4801 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4861 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4921 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4981 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5041 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5101 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5161 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5221 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5281 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5341 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     5401 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     5461 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     5521 atgccgcata gttaagccag taagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     5581 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     5641 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     5701 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     5761 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     5821 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     5881 ccacagccgg atctacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     5941 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6001 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6061 acgcgcgagg cagccagctt acctcccggt ggtgggtcgg tggtccctgg gcaggggtct
     6121 cccgatcccg gacgagcccc caaatgaaag acccccgctg acgggtagtc aatcactcag
     6181 aggagaccct cccaaggaac agcgagacca caagtcggat gcaactgcaa gagggtttat
     6241 tggatacacg ggtacccggg cgactcagtc aatcggagga ctggcgcccc gagtgagggg
     6301 ttgtgggctc ttttattgag ctcggggagc agaagcgcgc gaacagaagc gagaagcgaa
     6361 ctgattggtt agttcaaata aggcacaggg tcatttcagg tccttggggc accctggaaa
     6421 catctgatgg ttctctaggg tcgaccctgt tccatctgtt cctgaccttg atctgaactt
     6481 ctctattctc agttatgtat ttttccatgc cttgcaaaat ggcgttactt aagctagctt
     6541 gccaaaccta caggtggggt ctttcattcc cccctttttc tggagactaa ataaaatctt
     6601 ttattttatc tatggctcgt actctatagg cttcagctgg tgatattgtt gagtcaaaac
     6661 tagagcctgg accactgata tcctgtcttt aacaaattgg actaatcgat aagcttatct
     6721 ctacactggc tcgatggagt cagcctagtg tccctcggaa agccttgagg tccctttgga
     6781 gcagttctgc actcggcctc tgcagcgctg cagccctcga tagagatctg cacccaatcg
     6841 gcaggcacgg gcggcgatct ccaatctgcg ggatcagtca gatcacccga gtgcgtgggc
     6901 atgacaatcg tgccctgggg accaacacaa tccagaaggg cctgaatcac tgcgaccggc
     6961 cctcccgcga cccagccgag cgagcttagc gaactgtgga cgagaactgt gccaccaagc
     7021 gtaaggccgt tctctcgcat ttgccttgct aggctcgcgc gagttgctgg ctgaggcgtt
     7081 ctcgaaatca gctcttgttc ggtcggcatc tactctattc ctttgccctc ggacgagtgc
     7141 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
     7201 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
     7261 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
     7321 gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
     7381 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     7441 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     7501 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     7561 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     7621 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
     7681 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
     7741 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg
     7801 gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
     7861 ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa
     7921 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
     7981 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
     8041 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
     8101 acttctcgac agacgtcgcg gtgagttcag gctttccgga tctatccatc tttggtacgg
     8161 gttgtggcaa gcttatcatc gtgtttttca aaggaaaacc acgtccccgt ggttcggggg
     8221 gcctagacgt ttttttaacc tcgactaaac acatgtaaag catgtgcacc gaggccccag
     8281 atcagatccc atacaatggg gtaccttctg ggcatccttc agccccttgt tgaatacgct
     8341 tgaggagagc catttgactc tttccacaac tatccaactc acaacgtggc actggggttg
     8401 tgccgccttt gcaggtgtat cttatacacg tggcttttgg ccgcagaggc acctgtcgcc
     8461 aggtgggggg ttccgctgcc tgcaaagggt cgctacagac gttgtttgtc ttcaagaagc
     8521 ttccagagga actgcttcct tcacgacatt caacagacct tgcattcctt tggcgagagg
     8581 ggaaagaccc ctaggaatgc tcgtcaagaa gacagggcca ggtttccggg ccctcacatt
     8641 gccaaaagac ggcaatatgg tggaaaataa catatagaca aacgcacacc ggccttattc
     8701 caagcggctt cggccagtaa cgttaggggg ggggag
//
