LOCUS       sequence-384069-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_D80A in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_D80A
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(161..749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(241..260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(920..1711,1712..1780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1543..1562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1781..1885)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1960..1979)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2151..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2736..2754
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2785..2809
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2952..2976
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6796..6820)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6867..6890
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6927..6950
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6981..7007
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6981..7007
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7008..7355,7356..7358,7359..7967)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8011..8028)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8017..8241
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8374..8393)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8525..8572
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8580..9602
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8580..9602
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9732..9853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9769..9788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9823..9842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9902..9918)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9902..9918)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9915..9937)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9926..9942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9950..9956,9957..9974,9975..9980))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9995..10016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(join(10133..10143,1..7))
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
       61 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      601 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1201 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1501 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     1921 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     1981 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     2041 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     2101 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     2161 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2761 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2821 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     2881 agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc
     2941 tgcagatatc aacaagtttg tacaaaaaag ttggcatgtt cgtgttcctg gtgctgctgc
     3001 cactggtgag ctcccagtgc gtgaacctga ccaccagaac ccagctgcca cctgcctaca
     3061 ccaactcctt caccaggggc gtgtactacc cagacaaggt gttcagatcc agcgtgctgc
     3121 actctaccca ggacctgttc ctgccattct tctctaacgt gacctggttc cacgccatcc
     3181 acgtgtctgg aaccaacggc accaagagat tcgccaaccc agtgctgcca ttcaacgacg
     3241 gcgtgtactt cgcttctacc gagaagtcta acatcatcag gggttggatc ttcggaacca
     3301 ccctggactc taagacccag tctctgctga tcgtgaacaa cgctaccaac gtggtgatca
     3361 aggtgtgcga gttccagttc tgcaacgacc cattcctggg agtgtactac cacaagaaca
     3421 acaagtcttg gatggaaagc gagttcaggg tgtacagctc tgctaacaac tgcaccttcg
     3481 agtacgtgtc tcagcctttc ctgatggacc tggaaggaaa gcagggaaac ttcaagaacc
     3541 tgagagagtt cgtgttcaag aacatcgacg gttacttcaa gatatacagc aagcacaccc
     3601 caatcaacct ggtgagggac ctgccacagg gtttctctgc tctggagccc ctggtggacc
     3661 tgccaatcgg catcaacatc accaggttcc agaccctgct ggctctgcac aggtcttacc
     3721 tgaccccagg cgacagcagc tctggatgga ccgccggcgc cgctgcttac tacgtgggat
     3781 acctgcagcc aaggaccttc ctgctgaagt acaacgaaaa cggcaccatc accgacgccg
     3841 tggactgcgc tctggaccca ctgtctgaga ccaagtgcac cctgaagtct ttcaccgtgg
     3901 aaaagggaat ctaccagacc agcaacttcc gcgtgcagcc caccgagtct atcgtgagat
     3961 tcccaaacat caccaacctg tgcccattcg gagaagtgtt caacgctacc aggttcgcca
     4021 gcgtgtacgc ttggaacagg aagagaatca gcaactgcgt ggccgactac tctgtgctgt
     4081 acaactctgc ctccttctcc accttcaagt gctacggcgt gtccccaacc aagctgaacg
     4141 acctgtgctt caccaacgtg tacgctgact ccttcgtgat cagaggagac gaagtgagac
     4201 agatcgctcc cggtcagacc ggaaagatcg ccgactacaa ctacaagctg ccagacgact
     4261 tcaccggttg cgtgatcgct tggaactcta acaacctgga ctctaaggtg ggaggcaact
     4321 acaactacct gtacaggctg ttccgcaaga gcaacctgaa gcctttcgaa agggacatca
     4381 gcaccgaaat ctaccaggct ggatctaccc catgcaacgg agtggaagga ttcaactgct
     4441 acttccctct gcagtcttac ggattccagc caaccaacgg agtgggatac cagccataca
     4501 gagtggtggt gctgtctttc gagctgctgc acgctcctgc taccgtgtgc ggaccaaaga
     4561 agtctaccaa cctggtgaag aacaagtgcg tgaacttcaa cttcaacgga ctgaccggaa
     4621 ccggagtgct gaccgaatcc aacaagaagt tcctgccttt ccagcagttc ggtagagaca
     4681 tcgccgacac caccgacgct gtgagagacc cacagaccct ggaaatcctg gacatcaccc
     4741 cctgctcttt cggaggagtg agcgtgatca ccccaggaac caacacctcc aaccaggtgg
     4801 ccgtgctgta ccaggacgtg aactgcaccg aagtgccagt ggccatccac gctgaccagc
     4861 tgaccccaac ctggagagtg tacagcaccg gatctaacgt gttccagacc cgtgccggct
     4921 gcctgatcgg agccgagcac gtgaacaact cctacgaatg cgacatccca atcggagctg
     4981 gcatctgcgc ttcttaccag acccagacca acagcccaag aagggccaga tccgtggcct
     5041 ctcagagcat catcgcctac accatgagcc tgggcgctga aaacagcgtg gcttacagca
     5101 acaactctat cgccatccca accaacttca ccatctctgt gaccaccgaa atcctgccag
     5161 tgagcatgac caagaccagc gtggactgca ccatgtacat ctgcggagac tctaccgaat
     5221 gctctaacct gctgctgcag tacggcagct tctgcaccca gctgaaccgc gctctgaccg
     5281 gaatcgctgt ggagcaggac aagaacaccc aggaagtgtt cgctcaggtg aagcagatat
     5341 acaagacccc acctatcaag gacttcggag gattcaactt ctctcagatc ctgcccgacc
     5401 catctaagcc ctctaagagg tctttcatcg aagacctgct gttcaacaag gtgaccctgg
     5461 ccgacgccgg tttcatcaag cagtacggtg actgcctggg agacatcgcc gctagagacc
     5521 tgatctgcgc ccagaagttc aacggtctga ccgtgctgcc accactgctg accgacgaga
     5581 tgatcgccca gtacacctct gccctgctgg ctggaaccat cacctctggt tggaccttcg
     5641 gagctggcgc cgccctgcag atcccattcg ctatgcagat ggcctaccgt ttcaacggta
     5701 tcggagtgac ccagaacgtg ctgtacgaaa accagaagct gatcgctaac cagttcaact
     5761 ctgctatcgg aaagatccag gactccctgt cctctaccgc ttctgccctg ggaaagctgc
     5821 aggacgtggt gaaccagaac gcccaggccc tgaacaccct ggtgaagcag ctgtcttcta
     5881 acttcggagc catctcctct gtgctgaacg acatcctgtc taggctggac aaggtggaag
     5941 ccgaggtgca gatcgacaga ctgatcaccg gaagactgca gtctctgcag acctacgtga
     6001 cccagcagct gatcagggcc gctgaaatca gagcttctgc caacctggct gccaccaaga
     6061 tgtccgaatg cgtgctgggc cagtctaaga gggtggactt ctgcggaaag ggataccacc
     6121 tgatgtcctt cccacagtct gctccacacg gagtggtgtt cctgcacgtg acctacgtgc
     6181 ccgcccagga aaagaacttc accaccgccc cagctatctg ccacgacgga aaggctcact
     6241 tccccaggga gggagtgttc gtgtctaacg gaacccactg gttcgtgacc cagaggaact
     6301 tctacgagcc acagatcatc accaccgaca acaccttcgt gtctggaaac tgcgacgtgg
     6361 tgatcggaat cgtgaacaac accgtgtacg acccactgca gccagaactg gactctttca
     6421 aggaggaact ggacaagtac ttcaagaacc acacctctcc agacgtggac ctgggagaca
     6481 tctccggaat caacgcctct gtggtgaaca tccagaagga aatcgacaga ctgaacgagg
     6541 tggccaagaa cctgaacgaa tctctgatcg acctgcagga actgggaaag tacgagcagt
     6601 acatcaagtg gccctggtac atctggctgg gtttcatcgc tggtctgatc gctatcgtga
     6661 tggtgaccat catgctgtgc tgcatgacct cttgctgctc ttgcctgaag ggatgctgct
     6721 cttgcggatc ttgctgcaag ttcgacgagg acgactccga gcctgtgctg aagggtgtga
     6781 agctgcacta cacctaccca actttcttgt acaaagtggt tgatatcaag gccgccgaca
     6841 tcaccagcct gtacaagaaa gtggggtgga gccatcccca gttcgagaag ggcggaggat
     6901 ctggcggcgg aagcggcgga ggaagctggt cccaccctca gtttgagaaa ggcaccgagc
     6961 tgggcagcac catggccagc tacccctacg acgtgcccga ctacgccaag gacaacaccg
     7021 tgcccctgaa gctgatcgcc ctgctggcca acggcgagtt ccactctggc gagcagctgg
     7081 gcgagacact gggcatgagc agagccgcca tcaacaagca catccagacc ctgcgggact
     7141 ggggcgtgga cgtgttcaca gtgcccggca agggctacag cctgcccgag cccatccagc
     7201 tgctgaacgc caagcagatc ctgggccagc tggatggcgg atctgtggcc gtgctgcccg
     7261 tgatcgacag caccaaccag tatctgctcg accggatcgg cgagctgaag tccggcgacg
     7321 cctgtatcgc cgagtaccag caggccggca gaggcggcag gggcagaaag tggttcagcc
     7381 ccttcggcgc caacctgtac ctgagcatgt tctggagact ggaacagggc ccagccgccg
     7441 ctattggcct gagcctggtc atcggcatcg tgatggccga ggtgctgcgg aagctgggcg
     7501 ccgacaaagt gcgcgtgaag tggcccaacg acctgtatct gcaggaccgg aagctggccg
     7561 gcatcctggt ggaactgacc ggcaaaacag gcgacgccgc ccagatcgtg atcggcgctg
     7621 gcatcaacat ggccatgcgg cgggtggaag agagcgtggt caaccagggc tggatcaccc
     7681 tgcaggaagc cggcatcaac ctggaccgga acaccctggc cgccatgctg atcagagagc
     7741 tgagagccgc cctggaactg ttcgagcagg aaggcctggc cccctacctg agcagatggg
     7801 agaagctgga caacttcatc aaccggcccg tgaaactgat catcggcgac aaagagatct
     7861 tcggcatcag ccggggcatc gacaagcagg gggccctgct cctggaacag gacggcatca
     7921 tcaagccctg gatgggcggc gagatcagcc tgcggagcgc cgagaagtga tgactcgagt
     7981 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     8041 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     8101 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     8161 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     8221 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     8281 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8341 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8401 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8461 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8521 cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact tccttggcca
     8581 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     8641 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     8701 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     8761 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     8821 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     8881 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     8941 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     9001 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     9061 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     9121 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     9181 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     9241 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     9301 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     9361 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     9421 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     9481 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     9541 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     9601 agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     9661 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     9721 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9781 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9841 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     9901 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9961 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    10021 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    10081 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    10141 ctg
//
