LOCUS       sequence-38407-e         306 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: pAAV-minCMV-mCherry
FEATURES             Location/Qualifiers
     source          1..306
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      122..131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(145..169)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(166..204)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(222..241)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(223..241)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg
       61 tgcaccttga agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc
      121 accatggtgg cggtacctcc aggcgatctg acggttcact aaacgagctc tgcttatata
      181 ggcctcccac cgtacacgcc tacctcgaca tacgtggatc cccctatagt gagtcgtatt
      241 atctagaacg cgtgcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
      301 gcgctc
//
