LOCUS       sequence-384072-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_H655Y in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_H655Y
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(114..131)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(285..873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(365..384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1044..1835,1836..1904))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1667..1686
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1905..2009)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2084..2103)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2275..2654
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2655..2858
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2809..2829
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2860..2878
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2881..2899
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2909..2933
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3076..3100
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6920..6944)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6991..7014
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7051..7074
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7105..7131
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7105..7131
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7132..7479,7480..7482,7483..8091)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8135..8152)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8141..8365
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8498..8517)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8649..8696
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8704..9726
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8704..9726
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9856..9977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9893..9912)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9947..9966
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10026..10042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10026..10042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10039..10061)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10050..10066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10074..10080,10081..10098,10099..10104))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10119..10140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
       61 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
      121 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      181 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      241 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      721 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
      961 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1021 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     1081 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     1141 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     1201 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     1261 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     1321 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     1381 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1441 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1501 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1561 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1621 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     1681 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1741 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1801 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     1861 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     1921 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1981 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     2041 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     2101 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     2161 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     2221 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     2281 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     2341 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2401 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2461 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2521 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2581 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2641 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2701 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2761 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2821 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
     2881 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
     2941 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
     3001 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     3061 attctgcaga tatcaacaag tttgtacaaa aaagttggca tgttcgtgtt cctggtgctg
     3121 ctgccactgg tgagctccca gtgcgtgaac ctgaccacca gaacccagct gccacctgcc
     3181 tacaccaact ccttcaccag gggcgtgtac tacccagaca aggtgttcag atccagcgtg
     3241 ctgcactcta cccaggacct gttcctgcca ttcttctcta acgtgacctg gttccacgcc
     3301 atccacgtgt ctggaaccaa cggcaccaag agattcgaca acccagtgct gccattcaac
     3361 gacggcgtgt acttcgcttc taccgagaag tctaacatca tcaggggttg gatcttcgga
     3421 accaccctgg actctaagac ccagtctctg ctgatcgtga acaacgctac caacgtggtg
     3481 atcaaggtgt gcgagttcca gttctgcaac gacccattcc tgggagtgta ctaccacaag
     3541 aacaacaagt cttggatgga aagcgagttc agggtgtaca gctctgctaa caactgcacc
     3601 ttcgagtacg tgtctcagcc tttcctgatg gacctggaag gaaagcaggg aaacttcaag
     3661 aacctgagag agttcgtgtt caagaacatc gacggttact tcaagatata cagcaagcac
     3721 accccaatca acctggtgag ggacctgcca cagggtttct ctgctctgga gcccctggtg
     3781 gacctgccaa tcggcatcaa catcaccagg ttccagaccc tgctggctct gcacaggtct
     3841 tacctgaccc caggcgacag cagctctgga tggaccgccg gcgccgctgc ttactacgtg
     3901 ggatacctgc agccaaggac cttcctgctg aagtacaacg aaaacggcac catcaccgac
     3961 gccgtggact gcgctctgga cccactgtct gagaccaagt gcaccctgaa gtctttcacc
     4021 gtggaaaagg gaatctacca gaccagcaac ttccgcgtgc agcccaccga gtctatcgtg
     4081 agattcccaa acatcaccaa cctgtgccca ttcggagaag tgttcaacgc taccaggttc
     4141 gccagcgtgt acgcttggaa caggaagaga atcagcaact gcgtggccga ctactctgtg
     4201 ctgtacaact ctgcctcctt ctccaccttc aagtgctacg gcgtgtcccc aaccaagctg
     4261 aacgacctgt gcttcaccaa cgtgtacgct gactccttcg tgatcagagg agacgaagtg
     4321 agacagatcg ctcccggtca gaccggaaag atcgccgact acaactacaa gctgccagac
     4381 gacttcaccg gttgcgtgat cgcttggaac tctaacaacc tggactctaa ggtgggaggc
     4441 aactacaact acctgtacag gctgttccgc aagagcaacc tgaagccttt cgaaagggac
     4501 atcagcaccg aaatctacca ggctggatct accccatgca acggagtgga aggattcaac
     4561 tgctacttcc ctctgcagtc ttacggattc cagccaacca acggagtggg ataccagcca
     4621 tacagagtgg tggtgctgtc tttcgagctg ctgcacgctc ctgctaccgt gtgcggacca
     4681 aagaagtcta ccaacctggt gaagaacaag tgcgtgaact tcaacttcaa cggactgacc
     4741 ggaaccggag tgctgaccga atccaacaag aagttcctgc ctttccagca gttcggtaga
     4801 gacatcgccg acaccaccga cgctgtgaga gacccacaga ccctggaaat cctggacatc
     4861 accccctgct ctttcggagg agtgagcgtg atcaccccag gaaccaacac ctccaaccag
     4921 gtggccgtgc tgtaccagga cgtgaactgc accgaagtgc cagtggccat ccacgctgac
     4981 cagctgaccc caacctggag agtgtacagc accggatcta acgtgttcca gacccgtgcc
     5041 ggctgcctga tcggagccga gtacgtgaac aactcctacg aatgcgacat cccaatcgga
     5101 gctggcatct gcgcttctta ccagacccag accaacagcc caagaagggc cagatccgtg
     5161 gcctctcaga gcatcatcgc ctacaccatg agcctgggcg ctgaaaacag cgtggcttac
     5221 agcaacaact ctatcgccat cccaaccaac ttcaccatct ctgtgaccac cgaaatcctg
     5281 ccagtgagca tgaccaagac cagcgtggac tgcaccatgt acatctgcgg agactctacc
     5341 gaatgctcta acctgctgct gcagtacggc agcttctgca cccagctgaa ccgcgctctg
     5401 accggaatcg ctgtggagca ggacaagaac acccaggaag tgttcgctca ggtgaagcag
     5461 atatacaaga ccccacctat caaggacttc ggaggattca acttctctca gatcctgccc
     5521 gacccatcta agccctctaa gaggtctttc atcgaagacc tgctgttcaa caaggtgacc
     5581 ctggccgacg ccggtttcat caagcagtac ggtgactgcc tgggagacat cgccgctaga
     5641 gacctgatct gcgcccagaa gttcaacggt ctgaccgtgc tgccaccact gctgaccgac
     5701 gagatgatcg cccagtacac ctctgccctg ctggctggaa ccatcacctc tggttggacc
     5761 ttcggagctg gcgccgccct gcagatccca ttcgctatgc agatggccta ccgtttcaac
     5821 ggtatcggag tgacccagaa cgtgctgtac gaaaaccaga agctgatcgc taaccagttc
     5881 aactctgcta tcggaaagat ccaggactcc ctgtcctcta ccgcttctgc cctgggaaag
     5941 ctgcaggacg tggtgaacca gaacgcccag gccctgaaca ccctggtgaa gcagctgtct
     6001 tctaacttcg gagccatctc ctctgtgctg aacgacatcc tgtctaggct ggacaaggtg
     6061 gaagccgagg tgcagatcga cagactgatc accggaagac tgcagtctct gcagacctac
     6121 gtgacccagc agctgatcag ggccgctgaa atcagagctt ctgccaacct ggctgccacc
     6181 aagatgtccg aatgcgtgct gggccagtct aagagggtgg acttctgcgg aaagggatac
     6241 cacctgatgt ccttcccaca gtctgctcca cacggagtgg tgttcctgca cgtgacctac
     6301 gtgcccgccc aggaaaagaa cttcaccacc gccccagcta tctgccacga cggaaaggct
     6361 cacttcccca gggagggagt gttcgtgtct aacggaaccc actggttcgt gacccagagg
     6421 aacttctacg agccacagat catcaccacc gacaacacct tcgtgtctgg aaactgcgac
     6481 gtggtgatcg gaatcgtgaa caacaccgtg tacgacccac tgcagccaga actggactct
     6541 ttcaaggagg aactggacaa gtacttcaag aaccacacct ctccagacgt ggacctggga
     6601 gacatctccg gaatcaacgc ctctgtggtg aacatccaga aggaaatcga cagactgaac
     6661 gaggtggcca agaacctgaa cgaatctctg atcgacctgc aggaactggg aaagtacgag
     6721 cagtacatca agtggccctg gtacatctgg ctgggtttca tcgctggtct gatcgctatc
     6781 gtgatggtga ccatcatgct gtgctgcatg acctcttgct gctcttgcct gaagggatgc
     6841 tgctcttgcg gatcttgctg caagttcgac gaggacgact ccgagcctgt gctgaagggt
     6901 gtgaagctgc actacaccta cccaactttc ttgtacaaag tggttgatat caaggccgcc
     6961 gacatcacca gcctgtacaa gaaagtgggg tggagccatc cccagttcga gaagggcgga
     7021 ggatctggcg gcggaagcgg cggaggaagc tggtcccacc ctcagtttga gaaaggcacc
     7081 gagctgggca gcaccatggc cagctacccc tacgacgtgc ccgactacgc caaggacaac
     7141 accgtgcccc tgaagctgat cgccctgctg gccaacggcg agttccactc tggcgagcag
     7201 ctgggcgaga cactgggcat gagcagagcc gccatcaaca agcacatcca gaccctgcgg
     7261 gactggggcg tggacgtgtt cacagtgccc ggcaagggct acagcctgcc cgagcccatc
     7321 cagctgctga acgccaagca gatcctgggc cagctggatg gcggatctgt ggccgtgctg
     7381 cccgtgatcg acagcaccaa ccagtatctg ctcgaccgga tcggcgagct gaagtccggc
     7441 gacgcctgta tcgccgagta ccagcaggcc ggcagaggcg gcaggggcag aaagtggttc
     7501 agccccttcg gcgccaacct gtacctgagc atgttctgga gactggaaca gggcccagcc
     7561 gccgctattg gcctgagcct ggtcatcggc atcgtgatgg ccgaggtgct gcggaagctg
     7621 ggcgccgaca aagtgcgcgt gaagtggccc aacgacctgt atctgcagga ccggaagctg
     7681 gccggcatcc tggtggaact gaccggcaaa acaggcgacg ccgcccagat cgtgatcggc
     7741 gctggcatca acatggccat gcggcgggtg gaagagagcg tggtcaacca gggctggatc
     7801 accctgcagg aagccggcat caacctggac cggaacaccc tggccgccat gctgatcaga
     7861 gagctgagag ccgccctgga actgttcgag caggaaggcc tggcccccta cctgagcaga
     7921 tgggagaagc tggacaactt catcaaccgg cccgtgaaac tgatcatcgg cgacaaagag
     7981 atcttcggca tcagccgggg catcgacaag cagggggccc tgctcctgga acaggacggc
     8041 atcatcaagc cctggatggg cggcgagatc agcctgcgga gcgccgagaa gtgatgactc
     8101 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     8161 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     8221 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     8281 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     8341 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     8401 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     8461 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     8521 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8581 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8641 cgtacctaga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg
     8701 gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     8761 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     8821 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     8881 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     8941 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     9001 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     9061 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     9121 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     9181 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     9241 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     9301 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     9361 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     9421 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     9481 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     9541 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     9601 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     9661 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     9721 gaatagcacg tactacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     9781 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     9841 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9901 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9961 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
    10021 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    10081 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10141 att
//
