LOCUS       sequence-384077-        4390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes C-Venus followed by the parallel coiled-coil CC-Di..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Rhys GG, Cross JA, Dawson WM, Thompson HF, Shanmugaratnam S, Savery 
            NJ, Dodding MP, Hocker B, Woolfson DN
  TITLE     De novo designed peptides for cellular delivery and subcellular 
            localisation.
  JOURNAL   Nat Chem Biol. 2022 Jul 14. pii: 10.1038/s41589-022-01076-6. doi: 
            10.1038/s41589-022-01076-6.
  PUBMED    35836017
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 Jul 14. pii: 10.1038/s41589-022-01076-6. doi: 
            10.1038/s41589-022-01076-6."
COMMENT     Sequence Label: pBAD-C-Venus-CC-Di
FEATURES             Location/Qualifiers
     source          1..4390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     91..113
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(269..1147)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1174..1458
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1381..1400
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1504..1521
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1543..1794
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1731..1752
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1964..1981)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2134..2220
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2312..2339
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2358..2449
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2450..2518,2519..3310)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2668..2687)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3481..4069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3970..3989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4223..4240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    join(4255..4390,1..5)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4321..4340)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
       61 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
      121 cgtcatcacc gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg
      181 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
      241 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
      301 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
      361 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
      421 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
      481 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
      541 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
      601 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
      661 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
      721 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
      781 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
      841 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
      901 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
      961 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
     1021 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
     1081 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
     1141 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca
     1201 gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc
     1261 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa
     1321 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
     1381 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
     1441 tctctactgt ttctccatac ccgttttttg ggctaacagg aggaattaac catggggggt
     1501 tctcatcatc atcatcatca tggtatggct agcctgcagg gggacaagca gaagaacggc
     1561 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
     1621 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1681 ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1741 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcagc
     1801 ggcagcggca gcggtctaga gggtagtggc gaaattgctg cgctgaaaca ggaaatcgct
     1861 gccctgaaaa aagagaatgc cgctctgaaa tgggaaatcg cagcgctgaa acaaggttag
     1921 gtaccaagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat
     1981 cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc
     2041 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
     2101 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa
     2161 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     2221 ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc
     2281 ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt
     2341 gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2401 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2461 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2521 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2581 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2641 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     2701 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2761 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2821 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2881 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2941 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3001 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3061 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3121 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3181 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3241 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3301 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3361 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3421 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3481 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3541 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3601 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3661 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3721 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3781 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3841 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3901 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3961 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4021 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4081 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4141 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4201 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     4261 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     4321 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     4381 tgggtcatgg
//
