LOCUS       sequence-384080-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_L452R in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_L452R
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(127..143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(127..143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(140..162)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    151..167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(175..181,182..199,200..205))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    220..241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(358..375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(529..1117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(609..628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1288..2079,2080..2148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1911..1930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2149..2253)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2328..2347)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2519..2898
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2899..3102
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3053..3073
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3104..3122
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3125..3143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3153..3177
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3320..3344
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7164..7188)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7235..7258
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7295..7318
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7349..7375
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7349..7375
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7376..7723,7724..7726,7727..8335)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8379..8396)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8385..8609
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8742..8761)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8893..8940
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8948..9970
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8948..9970
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    join(10100..10143,1..78)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(join(10137..10143,1..13))
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
       61 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
      121 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      181 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      241 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      301 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      361 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      421 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      481 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      541 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      601 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      661 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      721 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      781 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      841 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      901 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      961 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1021 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1081 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1141 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1201 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1261 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1321 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1381 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     1441 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1501 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1561 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1621 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1681 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1741 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     1801 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     1861 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     1921 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     1981 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2041 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2101 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2161 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2221 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     2281 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     2341 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     2401 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     2461 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     2521 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     2581 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2641 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2701 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     2761 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2821 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2881 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     2941 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3001 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3061 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga
     3121 gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg tcagatcgcc
     3181 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     3241 cggactctag cgtttaaact taagcttggt accgagctcg gatccactag tccagtgtgg
     3301 tggaattctg cagatatcaa caagtttgta caaaaaagtt ggcatgttcg tgttcctggt
     3361 gctgctgcca ctggtgagct cccagtgcgt gaacctgacc accagaaccc agctgccacc
     3421 tgcctacacc aactccttca ccaggggcgt gtactaccca gacaaggtgt tcagatccag
     3481 cgtgctgcac tctacccagg acctgttcct gccattcttc tctaacgtga cctggttcca
     3541 cgccatccac gtgtctggaa ccaacggcac caagagattc gacaacccag tgctgccatt
     3601 caacgacggc gtgtacttcg cttctaccga gaagtctaac atcatcaggg gttggatctt
     3661 cggaaccacc ctggactcta agacccagtc tctgctgatc gtgaacaacg ctaccaacgt
     3721 ggtgatcaag gtgtgcgagt tccagttctg caacgaccca ttcctgggag tgtactacca
     3781 caagaacaac aagtcttgga tggaaagcga gttcagggtg tacagctctg ctaacaactg
     3841 caccttcgag tacgtgtctc agcctttcct gatggacctg gaaggaaagc agggaaactt
     3901 caagaacctg agagagttcg tgttcaagaa catcgacggt tacttcaaga tatacagcaa
     3961 gcacacccca atcaacctgg tgagggacct gccacagggt ttctctgctc tggagcccct
     4021 ggtggacctg ccaatcggca tcaacatcac caggttccag accctgctgg ctctgcacag
     4081 gtcttacctg accccaggcg acagcagctc tggatggacc gccggcgccg ctgcttacta
     4141 cgtgggatac ctgcagccaa ggaccttcct gctgaagtac aacgaaaacg gcaccatcac
     4201 cgacgccgtg gactgcgctc tggacccact gtctgagacc aagtgcaccc tgaagtcttt
     4261 caccgtggaa aagggaatct accagaccag caacttccgc gtgcagccca ccgagtctat
     4321 cgtgagattc ccaaacatca ccaacctgtg cccattcgga gaagtgttca acgctaccag
     4381 gttcgccagc gtgtacgctt ggaacaggaa gagaatcagc aactgcgtgg ccgactactc
     4441 tgtgctgtac aactctgcct ccttctccac cttcaagtgc tacggcgtgt ccccaaccaa
     4501 gctgaacgac ctgtgcttca ccaacgtgta cgctgactcc ttcgtgatca gaggagacga
     4561 agtgagacag atcgctcccg gtcagaccgg aaagatcgcc gactacaact acaagctgcc
     4621 agacgacttc accggttgcg tgatcgcttg gaactctaac aacctggact ctaaggtggg
     4681 aggcaactac aactaccggt acaggctgtt ccgcaagagc aacctgaagc ctttcgaaag
     4741 ggacatcagc accgaaatct accaggctgg atctacccca tgcaacggag tggaaggatt
     4801 caactgctac ttccctctgc agtcttacgg attccagcca accaacggag tgggatacca
     4861 gccatacaga gtggtggtgc tgtctttcga gctgctgcac gctcctgcta ccgtgtgcgg
     4921 accaaagaag tctaccaacc tggtgaagaa caagtgcgtg aacttcaact tcaacggact
     4981 gaccggaacc ggagtgctga ccgaatccaa caagaagttc ctgcctttcc agcagttcgg
     5041 tagagacatc gccgacacca ccgacgctgt gagagaccca cagaccctgg aaatcctgga
     5101 catcaccccc tgctctttcg gaggagtgag cgtgatcacc ccaggaacca acacctccaa
     5161 ccaggtggcc gtgctgtacc aggacgtgaa ctgcaccgaa gtgccagtgg ccatccacgc
     5221 tgaccagctg accccaacct ggagagtgta cagcaccgga tctaacgtgt tccagacccg
     5281 tgccggctgc ctgatcggag ccgagcacgt gaacaactcc tacgaatgcg acatcccaat
     5341 cggagctggc atctgcgctt cttaccagac ccagaccaac agcccaagaa gggccagatc
     5401 cgtggcctct cagagcatca tcgcctacac catgagcctg ggcgctgaaa acagcgtggc
     5461 ttacagcaac aactctatcg ccatcccaac caacttcacc atctctgtga ccaccgaaat
     5521 cctgccagtg agcatgacca agaccagcgt ggactgcacc atgtacatct gcggagactc
     5581 taccgaatgc tctaacctgc tgctgcagta cggcagcttc tgcacccagc tgaaccgcgc
     5641 tctgaccgga atcgctgtgg agcaggacaa gaacacccag gaagtgttcg ctcaggtgaa
     5701 gcagatatac aagaccccac ctatcaagga cttcggagga ttcaacttct ctcagatcct
     5761 gcccgaccca tctaagccct ctaagaggtc tttcatcgaa gacctgctgt tcaacaaggt
     5821 gaccctggcc gacgccggtt tcatcaagca gtacggtgac tgcctgggag acatcgccgc
     5881 tagagacctg atctgcgccc agaagttcaa cggtctgacc gtgctgccac cactgctgac
     5941 cgacgagatg atcgcccagt acacctctgc cctgctggct ggaaccatca cctctggttg
     6001 gaccttcgga gctggcgccg ccctgcagat cccattcgct atgcagatgg cctaccgttt
     6061 caacggtatc ggagtgaccc agaacgtgct gtacgaaaac cagaagctga tcgctaacca
     6121 gttcaactct gctatcggaa agatccagga ctccctgtcc tctaccgctt ctgccctggg
     6181 aaagctgcag gacgtggtga accagaacgc ccaggccctg aacaccctgg tgaagcagct
     6241 gtcttctaac ttcggagcca tctcctctgt gctgaacgac atcctgtcta ggctggacaa
     6301 ggtggaagcc gaggtgcaga tcgacagact gatcaccgga agactgcagt ctctgcagac
     6361 ctacgtgacc cagcagctga tcagggccgc tgaaatcaga gcttctgcca acctggctgc
     6421 caccaagatg tccgaatgcg tgctgggcca gtctaagagg gtggacttct gcggaaaggg
     6481 ataccacctg atgtccttcc cacagtctgc tccacacgga gtggtgttcc tgcacgtgac
     6541 ctacgtgccc gcccaggaaa agaacttcac caccgcccca gctatctgcc acgacggaaa
     6601 ggctcacttc cccagggagg gagtgttcgt gtctaacgga acccactggt tcgtgaccca
     6661 gaggaacttc tacgagccac agatcatcac caccgacaac accttcgtgt ctggaaactg
     6721 cgacgtggtg atcggaatcg tgaacaacac cgtgtacgac ccactgcagc cagaactgga
     6781 ctctttcaag gaggaactgg acaagtactt caagaaccac acctctccag acgtggacct
     6841 gggagacatc tccggaatca acgcctctgt ggtgaacatc cagaaggaaa tcgacagact
     6901 gaacgaggtg gccaagaacc tgaacgaatc tctgatcgac ctgcaggaac tgggaaagta
     6961 cgagcagtac atcaagtggc cctggtacat ctggctgggt ttcatcgctg gtctgatcgc
     7021 tatcgtgatg gtgaccatca tgctgtgctg catgacctct tgctgctctt gcctgaaggg
     7081 atgctgctct tgcggatctt gctgcaagtt cgacgaggac gactccgagc ctgtgctgaa
     7141 gggtgtgaag ctgcactaca cctacccaac tttcttgtac aaagtggttg atatcaaggc
     7201 cgccgacatc accagcctgt acaagaaagt ggggtggagc catccccagt tcgagaaggg
     7261 cggaggatct ggcggcggaa gcggcggagg aagctggtcc caccctcagt ttgagaaagg
     7321 caccgagctg ggcagcacca tggccagcta cccctacgac gtgcccgact acgccaagga
     7381 caacaccgtg cccctgaagc tgatcgccct gctggccaac ggcgagttcc actctggcga
     7441 gcagctgggc gagacactgg gcatgagcag agccgccatc aacaagcaca tccagaccct
     7501 gcgggactgg ggcgtggacg tgttcacagt gcccggcaag ggctacagcc tgcccgagcc
     7561 catccagctg ctgaacgcca agcagatcct gggccagctg gatggcggat ctgtggccgt
     7621 gctgcccgtg atcgacagca ccaaccagta tctgctcgac cggatcggcg agctgaagtc
     7681 cggcgacgcc tgtatcgccg agtaccagca ggccggcaga ggcggcaggg gcagaaagtg
     7741 gttcagcccc ttcggcgcca acctgtacct gagcatgttc tggagactgg aacagggccc
     7801 agccgccgct attggcctga gcctggtcat cggcatcgtg atggccgagg tgctgcggaa
     7861 gctgggcgcc gacaaagtgc gcgtgaagtg gcccaacgac ctgtatctgc aggaccggaa
     7921 gctggccggc atcctggtgg aactgaccgg caaaacaggc gacgccgccc agatcgtgat
     7981 cggcgctggc atcaacatgg ccatgcggcg ggtggaagag agcgtggtca accagggctg
     8041 gatcaccctg caggaagccg gcatcaacct ggaccggaac accctggccg ccatgctgat
     8101 cagagagctg agagccgccc tggaactgtt cgagcaggaa ggcctggccc cctacctgag
     8161 cagatgggag aagctggaca acttcatcaa ccggcccgtg aaactgatca tcggcgacaa
     8221 agagatcttc ggcatcagcc ggggcatcga caagcagggg gccctgctcc tggaacagga
     8281 cggcatcatc aagccctgga tgggcggcga gatcagcctg cggagcgccg agaagtgatg
     8341 actcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     8401 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8461 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8521 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8581 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     8641 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8701 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8761 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8821 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     8881 ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     8941 cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     9001 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     9061 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     9121 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     9181 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     9241 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     9301 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     9361 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     9421 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     9481 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     9541 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     9601 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     9661 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     9721 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     9781 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     9841 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     9901 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     9961 aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg aaaggttggg
    10021 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
    10081 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
    10141 tag
//
