LOCUS       sequence-384095-        4263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC47A1_STOP
FEATURES             Location/Qualifiers
     source          1..4263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1820..1919)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1936..1955)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1937..1955)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1960..1976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1960..1976)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2078..2097)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2089..2898
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2156..2175)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3045..3633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3534..3553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3787..3804
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3963..3990)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4082..4168)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4217..4239
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4231..4248
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4232..4248
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga ggcccctgag
      121 gagccagcac ctgtgagagg aggaccagag gccaccctgg aggtgagggg ctcccgctgc
      181 ctgcggctgt ctgccttcag agaggagctg agggccctgc tggtgctggc aggaccagcc
      241 tttctggtgc agctgatggt gttcctgatc tcttttatca gctccgtgtt ctgcggacac
      301 ctgggcaagc tggagctgga cgcagtgacc ctggcaatcg ccgtgatcaa cgtgacaggc
      361 gtgagcgtgg gctttggcct gtctagcgcc tgtgataccc tgatcagcca gacatacggc
      421 tcccagaatc tgaagcacgt gggcgtgatc ctgcagaggt ccgccctggt gctgctgctg
      481 tgctgtttcc catgttgggc cctgtttctg aacacccagc acatcctgct gctgttcaga
      541 caggaccccg acgtgagcag gctgacccag acatacgtga ccatcttcat ccctgccctg
      601 ccagccacat ttctgtacat gctgcaggtg aagtatctgc tgaatcaggg catcgtgctg
      661 ccacagatcg tgacaggagt ggcagccaac ctggtgaatg ccctggccaa ctatctgttc
      721 ctgcaccagc tgcacctggg cgtgatcgga agcgccctgg ccaacttgat ctctcaatac
      781 acgcttgcac tgctgctgtt tctgtatatc ctgggcaaga agctgcacca ggcaacatgg
      841 ggaggatgga gcctggagtg cctgcaggac tgggcatcct tcctgaggct ggcaatcccc
      901 tctatgctga tgctgtgcat ggagtggtgg gcctatgaag tgggcagctt tctgtccgga
      961 atcctgggaa tggtggagct gggagcacag agcatcgtgt acgagctggc catcatcgtg
     1021 tatatggtgc ctgccggctt ctccgtggcc gcctctgtgc gcgtgggaaa cgcactggga
     1081 gcaggcgaca tggagcaggc ccggaagtcc tctaccgtgt ccctgctgat cacagtgctg
     1141 ttcgccgtgg ccttttctgt gctgctgctg agctgcaagg atcacgtggg ctacatcttc
     1201 accacagacc gcgatatcat caatctggtg gcccaggtgg tgcctatcta tgccgtgagc
     1261 cacctgtttg aggccctggc ctgtacctct ggaggcgtgc tgaggggaag cggaaaccag
     1321 aaagtgggag ccatcgtgaa tacaatcggc tactatgtgg tgggcctgcc aatcggcatc
     1381 gccctgatgt tcgccaccac actgggcgtg atgggcctgt ggtccggcat catcatctgc
     1441 accgtgtttc aggccgtgtg cttcctgggc tttatcatcc agctgaactg gaagaaggca
     1501 tgccagcagg cacaggtgca cgccaacctg aaggtgaaca atgtgcctcg gtctggcaat
     1561 agcgccctgc cacaggaccc tctgcaccca ggatgtcccg agaacctgga gggcatcctg
     1621 accaatgacg tgggcaagac aggcgagccc cagagcgatc agcagatgag acaggaggag
     1681 ccactgccag agcaccctca ggatggagcc aagctgtcca ggaagcagct ggtgctgagg
     1741 agaggactgc tgctgctggg cgtgttcctg atcctgctgg tgggcatcct ggtgcgcttt
     1801 tacgtgcgga tccagtaaga cccagctttc ttgtacaaag ttggcattat aagaaagcat
     1861 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     1921 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1981 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     2041 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     2101 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     2161 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     2221 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     2281 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     2341 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     2401 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     2461 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     2521 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     2581 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     2641 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     2701 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     2761 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     2821 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     2881 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     2941 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3181 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3721 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3781 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     3841 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3901 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     3961 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     4021 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     4081 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4141 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     4201 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     4261 ccc
//
