LOCUS       sequence-384097-        7945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector to drive expression of moxBFP and Flpo under the control 
            of CamKIIalpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Liebsch F, Eggersmann FR, Merkler Y, Kloppenburg P, Schwarz G
  TITLE     Automated Image Analysis Reveals Different Localization of Synaptic 
            Gephyrin C4 Splice Variants.
  JOURNAL   eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.2022. doi: 
            10.1523/ENEURO.0102-22.2022. Print 2023 Jan.
  PUBMED    36543537
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/ENEURO.0102-22.2022"; journalName: "eNeuro"; date: 
            "2023-01-4- 4"; volume: "10"; issue: "1"; pages: 
            "ENEURO.0102-22.2022"
COMMENT     Sequence Label: pAAV-CamKIIa-moxBFP-IRES-Flpo
FEATURES             Location/Qualifiers
     source          1..7945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..609
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(74..94)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(492..503)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    641..1117
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(840..859)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   1157..1297
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1157..1286
                     /label=AAV2 ITR
     rep_origin      1372..1827
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1459..1478)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1669..1690
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1844..1863)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1963..1985
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2023..2041)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2109..2213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2214..2282,2283..3074)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2432..2451)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3245..3833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3734..3753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   3895..4024
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        4043..5331
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      5364..5373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5370..5372,5373..5375,5376..6089)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFISTTGKLPVPWPTLVTTLSHGVQVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSKLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(5415..5436)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    6106..6658
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6250..6267)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6477..6496
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
       61 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
      121 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
      181 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
      241 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggaa
      301 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
      361 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
      421 cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct
      481 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
      541 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
      601 cctccccgca tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc
      661 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
      721 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
      781 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt
      841 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
      901 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat
      961 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag
     1021 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg
     1081 attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg
     1141 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     1201 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     1261 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     1321 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     1381 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     1441 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     1501 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     1561 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     1621 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     1681 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
     1741 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     1801 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     1861 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     1921 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1981 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     2041 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2101 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2161 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     2221 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2281 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2341 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2401 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2461 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2521 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2581 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2641 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2701 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2761 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2821 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2881 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2941 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3001 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3061 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3121 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3181 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3241 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3301 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3361 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3421 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3481 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3541 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3601 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3661 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3721 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3781 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3841 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtcctgca
     3901 ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc gtcgggcgac ctttggtcgc
     3961 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt
     4021 tcctgcggcc gcacgcgttt aacattatgg ccttaggtca cttcatctcc atggggttct
     4081 tcttctgatt ttctagaaaa tgagatgggg gtgcagagag cttcctcagt gacctgccca
     4141 gggtcacatc agaaatgtca gagctagaac ttgaactcag attactaatc ttaaattcca
     4201 tgccttgggg gcatgcaagt acgatataca gaaggagtga actcattagg gcagatgacc
     4261 aatgagttta ggaaagaaga gtccagggca gggtacatct acaccacccg cccagccctg
     4321 ggtgagtcca gccacgttca cctcattata gttgcctctc tccagtccta ccttgacggg
     4381 aagcacaagc agaaactggg acaggagccc caggagacca aatcttcatg gtccctctgg
     4441 gaggatgggt ggggagagct gtggcagagg cctcaggagg ggccctgctg ctcagtggtg
     4501 acagataggg gtgagaaagc agacagagtc attccgtcag cattctgggt ctgtttggta
     4561 cttcttctca cgctaaggtg gcggtgtgat atgcacaatg gctaaaaagc agggagagct
     4621 ggaaagaaac aaggacagag acagaggcca agtcaaccag accaattccc agaggaagca
     4681 aagaaaccat tacagagact acaaggggga agggaaggag agatgaatta gcttcccctg
     4741 taaaccttag aacccagctg ttgccagggc aacggggcaa tacctgtctc ttcagaggag
     4801 atgaagttgc cagggtaact acatcctgtc tttctcaagg accatcccag aatgtggcac
     4861 ccactagccg ttaccatagc aactgcctct ttgccccact taatcccatc ccgtctgtta
     4921 aaagggccct atagttggag gtgggggagg taggaagagc gatgatcact tgtggactaa
     4981 gtttgttcgc atccccttct ccaaccccct cagtacatca ccctggggga acagggtcca
     5041 cttgctcctg ggcccacaca gtcctgcagt attgtgtata taaggccagg gcaaagagga
     5101 gcaggtttta aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa
     5161 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct
     5221 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcagtcaagc cggttctccg
     5281 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag ctctagagcg
     5341 gtaccggatc cccgggtacc ggtgccacca tggtgagcaa gggcgaggag ctgttcaccg
     5401 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtga
     5461 ggggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc atcagtacta
     5521 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgagccac ggcgtgcagg
     5581 tgttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     5641 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcacctac aagacccgcg
     5701 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcgtcgact
     5761 tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac agccacaaca
     5821 tctatatcat ggccgtcaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     5881 acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     5941 acggccccgt gctgctgccc gacagccact acctgagcac ccagtccaag cttagcaaag
     6001 accccaacga gaagcgcgat cacatggtcc tgctggagtt ccgcaccgcc gccgggatca
     6061 ctctcggcat ggacgagctg tacaagtaag gcgcgccccc ccctaacgtt actggccgaa
     6121 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     6181 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     6241 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     6301 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     6361 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     6421 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     6481 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     6541 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     6601 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     6661 gccacaacca tgagccagtt cgacatcctg tgcaagaccc cccccaaggt gctggtgcgg
     6721 cagttcgtgg agagattcga gaggcccagc ggcgagaaga tcgccagctg tgccgccgag
     6781 ctgacctacc tgtgctggat gatcacccac aacggcaccg ccatcaagag ggccaccttc
     6841 atgagctaca acaccatcat cagcaacagc ctgagcttcg acatcgtgaa caagagcctg
     6901 cagttcaagt acaagaccca gaaggccacc atcctggagg ccagcctgaa gaagctgatc
     6961 cccgcctggg agttcaccat catcccttac aacggccaga agcaccagag cgacatcacc
     7021 gacatcgtgt ccagcctgca gctgcagttc gagagcagcg aggaggccga caagggcaac
     7081 agccacagca agaagatgct gaaggccctg ctgtccgagg gcgagagcat ctgggagatc
     7141 accgagaaga tcctgaacag cttcgagtac accagcaggt tcaccaagac caagaccctg
     7201 taccagttcc tgttcctggc cacattcatc aactgcggca ggttcagcga catcaagaac
     7261 gtggacccca agagcttcaa gctggtgcag aacaagtacc tgggcgtgat cattcagtgc
     7321 ctggtgaccg agaccaagac aagcgtgtcc aggcacatct actttttcag cgccagaggc
     7381 aggatcgacc ccctggtgta cctggacgag ttcctgagga acagcgagcc cgtgctgaag
     7441 agagtgaaca ggaccggcaa cagcagcagc aacaagcagg agtaccagct gctgaaggac
     7501 aacctggtgc gcagctacaa caaggccctg aagaagaacg ccccctaccc catcttcgct
     7561 atcaagaacg gccctaagag ccacatcggc aggcacctga tgaccagctt tctgagcatg
     7621 aagggcctga ccgagctgac aaacgtggtg ggcaactgga gcgacaagag ggcctccgcc
     7681 gtggccagga ccacctacac ccaccagatc accgccatcc ccgaccacta cttcgccctg
     7741 gtgtccaggt actacgccta cgaccccatc agcaaggaga tgatcgccct gaaggacgag
     7801 accaacccca tcgaggagtg gcagcacatc gagcagctga agggcagcgc cgagggcagc
     7861 atcagatacc ccgcctggaa cggcatcatc agccaggagg tgctggacta cctgagcagc
     7921 tacatcaaca ggcggatctg agaat
//
