LOCUS       sequence-384102-       10707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Y635A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-eGFP-TAX1BP1 Y635A
FEATURES             Location/Qualifiers
     source          1..10707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      588..597
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(594..596,597..599,600..1310)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(594..596,597..599,600..1310)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(639..660)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(900..919)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1247..1268
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1326..1350
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3718..3742)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3761..4260
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3796..3819)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4193..4212
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4284..4682
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4724..4845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4761..4780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    4871..5459
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4924..4944)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5342..5353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5534..5767
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5886..5904)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5972..6076
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6077..6145,6146..6937)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6295..6314)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7108..7696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7597..7616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7850..7867
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8107..8128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8143..8148,8149..8166,8167..8173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8181..8197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8186..8208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8205..8221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8205..8221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             8482..9115
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9162..9287
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9784..10017
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10202..10246
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10539..10656
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgatctgcc accatggtga
      601 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      661 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      721 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      781 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      841 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      901 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      961 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1021 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     1081 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1141 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1201 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1261 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag gctcgaggtt
     1321 atcaaacaag tttgtacaaa aaagttggca tgacatcctt tcaagaagtc ccattgcaga
     1381 cttccaactt tgcccatgtc atctttcaaa atgtggccaa gagttacctt cctaatgcac
     1441 acctggaatg tcattacacc ttaactccat atattcatcc acatccaaaa gattgggttg
     1501 gtatattcaa ggttggatgg agtactgctc gtgattatta cacgttttta tggtccccta
     1561 tgcctgaaca ttatgtggaa ggatcaacag tcaattgtgt actagcattc caaggatatt
     1621 accttccaaa tgatgatgga gaattttatc agttctgtta cgttacccat aagggtgaaa
     1681 ttcgtggagc aagtacacct ttccagtttc gagcttcttc tccagttgaa gagctgctta
     1741 ctatggaaga tgaaggaaat tctgacatgt tagtggtgac cacaaaagca ggccttcttg
     1801 agttgaaaat tgagaaaacc atgaaagaaa aagaagaact gttaaagtta attgccgttc
     1861 tggaaaaaga aacagcacaa cttcgagaac aagttgggag aatggaaaga gaacttaacc
     1921 atgagaaaga aagatgtgac caactgcaag cagaacaaaa gggtcttact gaagtaacac
     1981 aaagcttaaa aatggaaaat gaagagttta agaagaggtt cagtgatgct acatccaaag
     2041 cccatcagct tgaggaagat attgtgtcag taacacataa agcaattgaa aaagaaaccg
     2101 aattagacag tttaaaggac aaactcaaga aggcacaaca tgaaagagaa caacttgaat
     2161 gtcagttgaa gacagagaag gatgaaaagg aactttataa ggtacatttg aagaatacag
     2221 aaatagaaaa taccaagctt atgtcagagg tccagacttt aaaaaattta gatgggaaca
     2281 aagaaagcgt gattactcat ttcaaagaag agattggcag gctgcagtta tgtttggctg
     2341 aaaaggaaaa tctgcaaaga actttcctgc ttacaacctc aagtaaagaa gatacttgtt
     2401 ttttaaagga gcaacttcgt aaagcagagg aacaggttca ggcaactcgg caagaagttg
     2461 tctttctggc taaagaactc agtgatgctg tcaacgtacg agacagaacg atggcagacc
     2521 tgcatactgc acgcttggaa aacgagaaag tgaaaaagca gttagctgat gcagtggcag
     2581 aacttaaact aaatgctatg aaaaaagatc aggacaagac tgatacactg gaacacgaac
     2641 taagaagaga agttgaagat ctgaaactcc gtcttcagat ggctgcagac cattataaag
     2701 aaaaatttaa ggaatgccaa aggctccaaa aacaaataaa caaactttca gatcaatcag
     2761 ctaataataa taatgtcttc acaaagaaaa cggggaatca gcagaaagtg aatgatgctt
     2821 cagtaaacac agacccagcc acttctgcct ctactgtaga tgtaaagcca tcaccttctg
     2881 cagcagaggc agattttgac atagtaacaa aggggcaagt ctgtgaaatg accaaagaaa
     2941 ttgctgacaa aacagaaaag tataataaat gtaaacaact cttgcaggat gagaaagcaa
     3001 aatgcaataa atatgctgat gaacttgcaa aaatggagct gaaatggaaa gaacaagtga
     3061 aaattgctga aaatgtaaaa cttgaactag ctgaagtaca ggacaattat aaagaactta
     3121 aaaggagtct agaaaatcca gcagaaagga aaatggaagg tcagaattcc cagagtcctc
     3181 aatgtttcaa aacatgctca gagcaaaatg gttatgttct cacattgtca aatgcacaac
     3241 cagttctgca agcaggtaat ccttatgcat ctcaggaaac aagagatgga gcagatggtg
     3301 ctttttaccc agatgaaata caaaggccac ctgtcagagt cccctcttgg ggactggaag
     3361 acaatgttgt ctgcagccag cctgctcgaa actttagtcg gcctgatggc ttagaggact
     3421 ctgaggatag caaagaagat gagaatgtgc ctactgctcc tgatcctcca agtcaacatt
     3481 tacgtgggca tgggacaggc ttttgctttg attccagctt tgatgttcac aagaagtgtc
     3541 ccctctgtga gttaatgttt cctcctaact atgatcagag caaatttgaa gaacatgttg
     3601 aaagtcactg gaaggtgtgc ccgatgtgca gcgagcagtt ccctcctgac tatgaccagc
     3661 aggtgtttga aaggcatgtg cagacccatt ttgatcagaa tgttctaaat tttgactacc
     3721 caactttctt gtacaaagtg gttcgataac gaattctacc gggtagggga ggcgcttttc
     3781 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     3841 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     3901 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     3961 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     4021 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     4081 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     4141 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     4201 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     4261 acctgcagcc cattaattaa accatggcca agcctttgtc tcaagaagaa tccaccctca
     4321 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     4381 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     4441 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     4501 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     4561 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     4621 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     4681 aagcacttcg tggccgagga gcaggactga cactcgacct cgaaacttgt ttattgcagc
     4741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctatgcatc
     4861 gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4921 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4981 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5041 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5101 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5161 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5221 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     5281 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5341 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5401 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     5461 tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5521 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     5581 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5641 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5701 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5761 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     5821 atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg
     5881 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5941 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6001 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6061 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6121 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6181 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6241 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6301 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6361 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6421 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6481 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6541 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6601 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6661 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6721 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6781 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6841 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6901 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6961 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7021 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7081 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7141 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7201 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7261 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7321 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7381 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7441 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7501 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7561 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7621 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7681 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7741 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7801 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7861 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7921 tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7981 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8041 tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     8101 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8161 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     8221 catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8281 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     8341 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     8401 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     8461 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     8521 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     8581 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     8641 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     8701 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     8761 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     8821 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     8881 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     8941 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9001 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9061 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     9121 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     9181 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     9241 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     9301 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     9361 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     9421 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     9481 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     9541 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     9601 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     9661 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     9721 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     9781 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     9841 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     9901 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     9961 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    10021 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10081 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    10141 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    10201 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    10261 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    10321 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    10381 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
    10441 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
    10501 gtatcgccga attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
    10561 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
    10621 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
    10681 gatccagttt ggactagtgg agttccg
//
