LOCUS       sequence-384108-       11310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral delivery of Traditional CAR(anti-CD19).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Li HS, Wong NM, Tague E, Ngo JT, Khalil AS, Wong WW
  TITLE     High-performance multiplex drug-gated CAR circuits.
  JOURNAL   Cancer Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008.
  PUBMED    36084652
REFERENCE   2  (bases 1 to 11310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008."
COMMENT     Sequence Label: pHR-Traditional CAR (anti-CD19)
FEATURES             Location/Qualifiers
     source          1..11310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      32..167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1315..1380
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1510..1530
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1955..2089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1992..2011)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2046..2065
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2257..2890
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2937..3062
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3559..3792
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3977..4021
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4306..4423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4497..5675
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4728..5666
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5623..5643
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5712..5721
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6507..6548
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7430..8018
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7483..7503)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7901..7912)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8021..8037)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8022..8038)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8128..8361
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8386..8404)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8387..8404)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8694..8713)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8927..8946)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9046..9068
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9106..9124)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9192..9296
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9297..9365,9366..10157)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9515..9534)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10328..10916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10817..10836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11070..11087
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11157..11177)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(11162..11310,1..181)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
       61 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
      121 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
      181 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
      241 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
      301 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
      361 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
      421 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
      481 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
      541 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
      601 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
      661 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
      721 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
      781 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
      841 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
      901 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
      961 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     1021 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     1081 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     1141 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     1201 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     1261 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     1321 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     1381 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     1441 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     1501 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     1561 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     1621 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     1681 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     1741 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     1801 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     1861 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     1921 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     1981 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2041 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     2101 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     2161 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     2221 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     2281 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     2341 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     2401 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     2461 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     2521 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     2581 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     2641 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     2701 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2761 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     2821 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     2881 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     2941 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     3001 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     3061 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     3121 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     3181 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     3241 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     3301 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     3361 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     3421 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     3481 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     3541 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     3601 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     3661 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     3721 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     3781 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     3841 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     3901 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     3961 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     4021 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     4081 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     4141 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     4201 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     4261 gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg
     4321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     4381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     4441 cagcagagat ccagtttgga tcgataagct tgatatcgaa ttcctgcagc ccgtgaggct
     4501 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     4561 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     4621 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     4681 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     4741 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     4801 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     4861 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     4921 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     4981 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     5041 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     5101 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     5161 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     5221 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     5281 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     5341 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     5401 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     5461 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     5521 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     5581 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     5641 ttcaaagttt ttttcttcca tttcaggtgt cgtgagggga tctggagctc tcgagaattc
     5701 tcacgcgtgc cgccaccatg gccttaccag tgaccgcctt gctcctgccg ctggccttgc
     5761 tgctccacgc cgccaggccg gacatccaga tgacacagac tacatcctcc ctgtctgcct
     5821 ctctgggaga cagagtcacc atcagttgca gggcaagtca ggacattagt aaatatttaa
     5881 attggtatca gcagaaacca gatggaactg ttaaactcct gatctaccat acatcaagat
     5941 tacactcagg agtcccatca aggttcagtg gcagtgggtc tggaacagat tattctctca
     6001 ccattagcaa cctggagcaa gaagatattg ccacttactt ttgccaacag ggtaatacgc
     6061 ttccgtacac gttcggaggc ggcaccaagc tggagatcac aggtggcggt ggctcgggcg
     6121 gtggtgggtc gggtggcggc ggatctgagg tgaaactgca ggagtcagga cctggcctgg
     6181 tggcgccctc acagagcctg tccgtcacat gcactgtctc aggggtctca ttacccgact
     6241 atggtgtaag ctggattcgc cagcctccac gaaagggtct ggagtggctg ggagtaatat
     6301 ggggtagtga aaccacatac tataattcag ctctcaaatc cagactgacc atcatcaagg
     6361 acaactccaa gagccaagtt ttcttaaaaa tgaacagtct gcaaactgat gacacagcca
     6421 tttactactg tgccaaacat tattactacg gtggtagcta tgctatggac tactggggcc
     6481 aaggaacctc agtcaccgtc tcctcaggga agcctatccc gaaccctctg ttgggtctcg
     6541 atagtaccaa tggggccgca accacgacgc cagcgccgcg accaccaaca ccggcgccca
     6601 ccatcgcgtt gcagcccctg tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg
     6661 cagtgcacac gagggggctg gacttcgcct gtgatttttg ggtgctggtg gtggttggtg
     6721 gagtcctggc ttgctatagc ttgctagtaa cagtggcctt tattattttc tgggtgagga
     6781 gtaagaggag caggctcctg cacagtgact acatgaacat gactccccgc cgccccgggc
     6841 ccacccgcaa gcattaccag ccctatgccc caccacgcga cttcgcagcc tatcgctcca
     6901 aacggggcag aaagaaactc ctgtatatat tcaaacaacc atttatgaga ccagtacaaa
     6961 ctactcaaga ggaagatggc tgtagctgcc gatttccaga agaagaagaa ggaggatgtg
     7021 aactgagagt gaagttcagc aggagcgcag acgcccccgc gtacaagcag ggccagaacc
     7081 agctctataa cgagctcaat ctaggacgaa gagaggagta cgatgttttg gacaagagac
     7141 gtggccggga ccctgagatg gggggaaagc cgagaaggaa gaaccctcag gaaggcctgt
     7201 acaatgaact gcagaaagat aagatggcgg aggcctacag tgagattggg atgaaaggcg
     7261 agcgccggag gggcaagggg cacgatggcc tttaccaggg tctcagtaca gccaccaagg
     7321 acacctacga cgcccttcac atgcaggccc tgccccctcg ctaaactagt gcggccgcga
     7381 ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct
     7441 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7501 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7561 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7621 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7681 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7741 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7801 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     7861 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7921 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7981 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg
     8041 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     8101 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8161 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8221 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8281 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8341 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct
     8401 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
     8461 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc
     8521 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt
     8581 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta
     8641 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     8701 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     8761 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     8821 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     8881 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg
     8941 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     9001 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     9061 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     9121 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     9181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9301 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     9361 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     9421 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     9481 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     9541 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     9601 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     9661 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     9721 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     9781 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     9841 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     9901 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9961 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10021 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    10081 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10141 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    10201 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    10261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    10321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    10381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    10441 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    10501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    10561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    10621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    10681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    10741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    10801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    10861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    11041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    11101 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
    11161 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    11221 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
    11281 aagtatgcaa agcatgcatc tcaattagtc
//
