LOCUS       sequence-384118-       13143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate 
            design(d+i).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13143)
  AUTHORS   Li HS, Wong NM, Tague E, Ngo JT, Khalil AS, Wong WW
  TITLE     High-performance multiplex drug-gated CAR circuits.
  JOURNAL   Cancer Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008.
  PUBMED    36084652
REFERENCE   2  (bases 1 to 13143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008."
COMMENT     Sequence Label: pHR-OFF VIPER CAR (anti-CD19)-candidate design...
FEATURES             Location/Qualifiers
     source          1..13143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1258..1323
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1453..1473
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1898..2032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1935..1954)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1989..2008
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2200..2833
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2880..3005
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3502..3735
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3920..3964
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4249..4366
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4440..5618
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4671..5609
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5566..5586
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5655..5664
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6450..6491
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7122..7178
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8436..9146
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8581..8599)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8849..8868
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    9206..9794
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9259..9279)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9677..9688)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9797..9813)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9798..9814)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9904..10137
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10162..10180)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10163..10180)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10470..10489)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10703..10722)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10822..10844
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10882..10900)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10968..11072
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11073..11141,11142..11933)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11291..11310)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12104..12692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12593..12612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12846..12863
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12933..12953)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(12938..13143,1..124)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(13118..13143,1..110)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
       61 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      121 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
      181 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
      241 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
      301 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
      361 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
      421 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
      481 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
      541 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
      601 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
      661 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
      721 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
      781 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
      841 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
      901 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
      961 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     1021 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     1081 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     1141 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     1201 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     1261 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     1321 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     1381 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     1441 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     1501 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     1561 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     1621 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     1681 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     1741 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
     1801 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     1861 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     1921 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1981 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     2041 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     2101 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     2161 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     2221 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     2281 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     2341 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     2401 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     2461 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     2521 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     2581 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     2641 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     2701 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     2761 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     2821 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     2881 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     2941 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     3001 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     3061 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     3121 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     3181 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     3241 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     3301 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     3361 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     3421 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     3481 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     3541 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     3601 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     3661 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     3721 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     3781 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     3841 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     3901 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     3961 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     4021 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     4081 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     4141 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     4201 agtgaacgga tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa
     4261 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     4321 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     4381 ggacagcaga gatccagttt ggatcgataa gcttgatatc gaattcctgc agcccgtgag
     4441 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     4501 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     4561 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     4621 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     4681 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     4741 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     4801 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     4861 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     4921 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     4981 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     5041 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     5101 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     5161 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     5221 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     5281 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     5341 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     5401 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     5461 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     5521 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     5581 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg ggatctggag ctctcgagaa
     5641 ttctcacgcg tgccgccacc atggccttac cagtgaccgc cttgctcctg ccgctggcct
     5701 tgctgctcca cgccgccagg ccggacatcc agatgacaca gactacatcc tccctgtctg
     5761 cctctctggg agacagagtc accatcagtt gcagggcaag tcaggacatt agtaaatatt
     5821 taaattggta tcagcagaaa ccagatggaa ctgttaaact cctgatctac catacatcaa
     5881 gattacactc aggagtccca tcaaggttca gtggcagtgg gtctggaaca gattattctc
     5941 tcaccattag caacctggag caagaagata ttgccactta cttttgccaa cagggtaata
     6001 cgcttccgta cacgttcgga ggcggcacca agctggagat cacaggtggc ggtggctcgg
     6061 gcggtggtgg gtcgggtggc ggcggatctg aggtgaaact gcaggagtca ggacctggcc
     6121 tggtggcgcc ctcacagagc ctgtccgtca catgcactgt ctcaggggtc tcattacccg
     6181 actatggtgt aagctggatt cgccagcctc cacgaaaggg tctggagtgg ctgggagtaa
     6241 tatggggtag tgaaaccaca tactataatt cagctctcaa atccagactg accatcatca
     6301 aggacaactc caagagccaa gttttcttaa aaatgaacag tctgcaaact gatgacacag
     6361 ccatttacta ctgtgccaaa cattattact acggtggtag ctatgctatg gactactggg
     6421 gccaaggaac ctcagtcacc gtctcctcag ggaagcctat cccgaaccct ctgttgggtc
     6481 tcgatagtac caatggggcc gcaaccacga cgccagcgcc gcgaccacca acaccggcgc
     6541 ccaccatcgc gttgcagccc ctgtccctgc gcccagaggc gtgccggcca gcggcggggg
     6601 gcgcagtgca cacgaggggg ctggacttcg cctgtgattt ttgggtgctg gtggtggttg
     6661 gtggagtcct ggcttgctat agcttgctag taacagtggc ctttattatt ttctgggtga
     6721 ggagtaagag gagcaggctc ctgcacagtg actacatgaa catgactccc cgccgccccg
     6781 ggcccacccg caagcattac cagccctatg ccccaccacg cgacttcgca gcctatcgct
     6841 ccaaacgggg cagaaagaaa ctcctgtata tattcaaaca accatttatg agaccagtac
     6901 aaactactca agaggaagat ggctgtagct gccgatttcc agaagaagaa gaaggaggat
     6961 gtgaactggg gggtaccgaa ttcagcgggt caggcggatc tggaagtggt ggtggagaac
     7021 ttgatgaatt ggtatactta ctagatgggc caggttatga ccctatacat tgcgatgtag
     7081 tgacaagggg cggcagccac cttttcaatt ttggaagcgg agctactaac ttcagcctgc
     7141 tgaagcaggc tggagacgtg gaggagaacc ctggacctat gattcatctt ggtcacatcc
     7201 tcttcttgct gctgttgcca gttgctgccg cacaaacaac cccaggtgag aggtcaagtc
     7261 ttcccgcctt ctatccgggc acaagcggga gttgtagtgg gtgtggatct ttgtcccttc
     7321 ctatatacat ctgggctcct ctggctggca cttgcggagt gcttctgctg agtctggtta
     7381 ttaccaaacg gggcagaaag aaactcctgt atatattcaa acaaccattt atgagaccag
     7441 tacaaactac tcaagaggaa gatggctgta gctgccgatt tccagaagaa gaagaaggag
     7501 gatgtgaact gggttctgtt gttattgttg gtagaattat tttatctggt agtggtagta
     7561 tcacggccta ctcccaacag acgcggggcc tacttggttg catcatcact agcctcacag
     7621 gccgggacaa gaaccaggtc gaaggggagg ttcaagtggt ttctaccgca acacaatctt
     7681 tcctggcgac ctgcgtcaac ggcgtgtgct ggactgtcta ccatggcgct ggctcgaaga
     7741 ccctagccgg tccaaaaggt ccaatcaccc aaatgtacac caatgtagac caggacctcg
     7801 tcggctggca ggcgcctcca ggggcgcgct ccttgacacc atgcacctgt ggcagctcgg
     7861 acctttactt ggtcacgaga catgctgatg tcattccggt gcgccggcga ggcgacagca
     7921 ggggaagtct actctccccc aggcccgtct cctacctgaa aggctccgca ggtggtccat
     7981 tgctttgccc ttcggggcac gctgtgggca tcttccgggc tgctgtgtgc acccgggggg
     8041 tcgcgaaggc ggtggacttc gtgcccgttg agtctatgga aactaccatg cggtctagag
     8101 tgaagttcag caggagcgca gacgcccccg cgtacaagca gggccagaac cagctctata
     8161 acgagctcaa tctaggacga agagaggagt acgatgtttt ggacaagaga cgtggccggg
     8221 accctgagat ggggggaaag ccgagaagga agaaccctca ggaaggcctg tacaatgaac
     8281 tgcagaaaga taagatggcg gaggcctaca gtgagattgg gatgaaaggc gagcgccgga
     8341 ggggcaaggg gcacgatggc ctttaccagg gtctcagtac agccaccaag gacacctacg
     8401 acgcccttca catgcaggcc ctgccccctc gcggcatggt gagcaagggc gaggaggata
     8461 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     8521 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     8581 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     8641 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     8701 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     8761 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     8821 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     8881 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     8941 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     9001 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     9061 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     9121 gcggcatgga cgagctgtac aagtaagcgg ccgcgactct agagtcgacc tgcaggcatg
     9181 caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     9241 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     9301 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     9361 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     9421 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     9481 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     9541 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     9601 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     9661 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     9721 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     9781 ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat taattcgagc tcggtacctt
     9841 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     9901 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     9961 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    10021 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    10081 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
    10141 tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa
    10201 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc
    10261 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
    10321 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga
    10381 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat
    10441 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
    10501 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
    10561 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
    10621 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
    10681 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
    10741 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
    10801 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
    10861 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
    10921 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10981 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    11041 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    11101 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    11161 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    11221 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    11281 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    11341 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    11401 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    11461 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    11521 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    11581 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    11641 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11701 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11761 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11821 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11881 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11941 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    12001 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    12061 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    12121 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    12181 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    12241 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    12301 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    12361 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    12421 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    12481 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    12541 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    12601 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12661 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12721 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    12781 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    12841 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    12901 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
    12961 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    13021 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    13081 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    13141 ttc
//
