LOCUS       sequence-384119-       13017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Li HS, Wong NM, Tague E, Ngo JT, Khalil AS, Wong WW
  TITLE     High-performance multiplex drug-gated CAR circuits.
  JOURNAL   Cancer Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008.
  PUBMED    36084652
REFERENCE   2  (bases 1 to 13017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008."
COMMENT     Sequence Label: pHR-OFF VIPER CAR (anti-CD19)-candidate
            design...
FEATURES             Location/Qualifiers
     source          1..13017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..147
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1295..1360
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1490..1510
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1935..2069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1972..1991)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2026..2045
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2237..2870
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2917..3042
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3539..3772
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3957..4001
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4286..4403
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4477..5655
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4708..5646
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5603..5623
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5692..5701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6487..6528
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7159..7215
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8347..9057
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8492..8510)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8760..8779
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    9117..9705
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9170..9190)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9588..9599)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9708..9724)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9709..9725)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9815..10048
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10073..10091)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10074..10091)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10381..10400)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10614..10633)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10733..10755
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10793..10811)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10879..10983
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10984..11052,11053..11844)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11202..11221)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12015..12603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12504..12523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12757..12774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12844..12864)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(12849..13017,1..161)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
       61 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
      121 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
      181 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
      241 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
      301 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
      361 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
      421 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
      481 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
      541 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
      601 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
      661 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
      721 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
      781 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
      841 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
      901 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
      961 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     1021 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     1081 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
     1141 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
     1201 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     1261 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     1321 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     1381 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     1441 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     1501 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     1561 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     1621 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     1681 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     1741 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     1801 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     1861 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     1921 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1981 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2041 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     2101 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     2161 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     2221 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     2281 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     2341 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     2401 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     2461 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     2521 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     2581 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     2641 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     2701 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     2761 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     2821 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     2881 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     2941 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     3001 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     3061 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     3121 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     3181 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     3241 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     3301 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     3361 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     3421 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     3481 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     3541 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     3601 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     3661 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     3721 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     3781 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     3841 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     3901 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     3961 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     4021 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     4081 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     4141 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     4201 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     4261 gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     4321 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     4381 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
     4441 tcgataagct tgatatcgaa ttcctgcagc ccgtgaggct ccggtgcccg tcagtgggca
     4501 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
     4561 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4621 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     4681 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     4741 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     4801 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     4861 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     4921 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     4981 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     5041 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     5101 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     5161 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     5221 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     5281 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     5341 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     5401 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     5461 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     5521 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     5581 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     5641 tttcaggtgt cgtgagggga tctggagctc tcgagaattc tcacgcgtgc cgccaccatg
     5701 gccttaccag tgaccgcctt gctcctgccg ctggccttgc tgctccacgc cgccaggccg
     5761 gacatccaga tgacacagac tacatcctcc ctgtctgcct ctctgggaga cagagtcacc
     5821 atcagttgca gggcaagtca ggacattagt aaatatttaa attggtatca gcagaaacca
     5881 gatggaactg ttaaactcct gatctaccat acatcaagat tacactcagg agtcccatca
     5941 aggttcagtg gcagtgggtc tggaacagat tattctctca ccattagcaa cctggagcaa
     6001 gaagatattg ccacttactt ttgccaacag ggtaatacgc ttccgtacac gttcggaggc
     6061 ggcaccaagc tggagatcac aggtggcggt ggctcgggcg gtggtgggtc gggtggcggc
     6121 ggatctgagg tgaaactgca ggagtcagga cctggcctgg tggcgccctc acagagcctg
     6181 tccgtcacat gcactgtctc aggggtctca ttacccgact atggtgtaag ctggattcgc
     6241 cagcctccac gaaagggtct ggagtggctg ggagtaatat ggggtagtga aaccacatac
     6301 tataattcag ctctcaaatc cagactgacc atcatcaagg acaactccaa gagccaagtt
     6361 ttcttaaaaa tgaacagtct gcaaactgat gacacagcca tttactactg tgccaaacat
     6421 tattactacg gtggtagcta tgctatggac tactggggcc aaggaacctc agtcaccgtc
     6481 tcctcaggga agcctatccc gaaccctctg ttgggtctcg atagtaccaa tggggccgca
     6541 accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtt gcagcccctg
     6601 tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg
     6661 gacttcgcct gtgatttttg ggtgctggtg gtggttggtg gagtcctggc ttgctatagc
     6721 ttgctagtaa cagtggcctt tattattttc tgggtgagga gtaagaggag caggctcctg
     6781 cacagtgact acatgaacat gactccccgc cgccccgggc ccacccgcaa gcattaccag
     6841 ccctatgccc caccacgcga cttcgcagcc tatcgctcca aacggggcag aaagaaactc
     6901 ctgtatatat tcaaacaacc atttatgaga ccagtacaaa ctactcaaga ggaagatggc
     6961 tgtagctgcc gatttccaga agaagaagaa ggaggatgtg aactgggggg taccgaattc
     7021 agcgggtcag gcggatctgg aagtggtggt ggagaacttg atgaattggt atacttacta
     7081 gatgggccag gttatgaccc tatacattgc gatgtagtga caaggggcgg cagccacctt
     7141 ttcaattttg gaagcggagc tactaacttc agcctgctga agcaggctgg agacgtggag
     7201 gagaaccctg gacctatgat tcatcttggt cacatcctct tcttgctgct gttgccagtt
     7261 gctgccgcac aaacaacccc aggtgagagg tcaagtcttc ccgccttcta tccgggcaca
     7321 agcgggagtt gtagtgggtg tggatctttg tcccttccta tatacatctg ggctcctctg
     7381 gctggcactt gcggagtgct tctgctgagt ctggttatta ccggttctgt tgttattgtt
     7441 ggtagaatta ttttatctgg tagtggtagt atcacggcct actcccaaca gacgcggggc
     7501 ctacttggtt gcatcatcac tagcctcaca ggccgggaca agaaccaggt cgaaggggag
     7561 gttcaagtgg tttctaccgc aacacaatct ttcctggcga cctgcgtcaa cggcgtgtgc
     7621 tggactgtct accatggcgc tggctcgaag accctagccg gtccaaaagg tccaatcacc
     7681 caaatgtaca ccaatgtaga ccaggacctc gtcggctggc aggcgcctcc aggggcgcgc
     7741 tccttgacac catgcacctg tggcagctcg gacctttact tggtcacgag acatgctgat
     7801 gtcattccgg tgcgccggcg aggcgacagc aggggaagtc tactctcccc caggcccgtc
     7861 tcctacctga aaggctccgc aggtggtcca ttgctttgcc cttcggggca cgctgtgggc
     7921 atcttccggg ctgctgtgtg cacccggggg gtcgcgaagg cggtggactt cgtgcccgtt
     7981 gagtctatgg aaactaccat gcggtctaga gtgaagttca gcaggagcgc agacgccccc
     8041 gcgtacaagc agggccagaa ccagctctat aacgagctca atctaggacg aagagaggag
     8101 tacgatgttt tggacaagag acgtggccgg gaccctgaga tggggggaaa gccgagaagg
     8161 aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag ataagatggc ggaggcctac
     8221 agtgagattg ggatgaaagg cgagcgccgg aggggcaagg ggcacgatgg cctttaccag
     8281 ggtctcagta cagccaccaa ggacacctac gacgcccttc acatgcaggc cctgccccct
     8341 cgcggcatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     8401 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     8461 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     8521 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     8581 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     8641 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     8701 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     8761 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     8821 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     8881 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     8941 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     9001 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagcg
     9061 gccgcgactc tagagtcgac ctgcaggcat gcaagcttga tatcaagctt atcgataatc
     9121 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     9181 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     9241 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     9301 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     9361 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     9421 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     9481 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     9541 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     9601 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     9661 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     9721 ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     9781 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     9841 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     9901 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     9961 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10021 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
    10081 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
    10141 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
    10201 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
    10261 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
    10321 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
    10381 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
    10441 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
    10501 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
    10561 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
    10621 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
    10681 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
    10741 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
    10801 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    10861 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    10921 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    10981 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    11041 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    11101 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    11161 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    11221 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    11281 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    11341 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    11401 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    11461 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    11521 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    11581 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    11641 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    11701 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    11761 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    11821 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    11881 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    11941 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    12001 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    12061 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    12121 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    12181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    12241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    12301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    12361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    12421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    12481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    12541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    12601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    12661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    12721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    12781 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    12841 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
    12901 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
    12961 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccc
//
