LOCUS       sequence-384121-        5223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red Fluorescent Reporter of intracellular hydrogen peroxide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Kardashliev T, Weingartner A, Romero E, Schwaneberg U, Fraaije M, 
            Panke S, Held M
  TITLE     Whole-cell screening of oxidative enzymes using genetically encoded 
            sensors.
  JOURNAL   Chem Sci. 2021 Oct 29;12(44):14766-14772. doi: 10.1039/d1sc02578c. 
            eCollection 2021 Nov 17.
  PUBMED    34820092
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/d1sc02578c"; journalName: "Chem Sci"; date: "2021-10-29- 
            29"; volume: "12"; issue: "44"; pages: "14766-14772"
COMMENT     Sequence Label: pOxyR_rfp
FEATURES             Location/Qualifiers
     source          1..5223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..951
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     WTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     oriT            1366..1474
                     /note="incP origin of transfer"
     CDS             complement(1620..2411)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      complement(2647..2741)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             3804..4511
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MHSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3949..3967)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4217..4236
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      4535..4621
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      4954..5176
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta tgacttaacg
       61 gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac gattgaaaac
      121 cctacaagga aagaatggac ggtatcgttc acttataacc aatacgctca gatgatgaac
      181 atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct gatgacgaga
      241 actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg gacaaactat
      301 gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt gccttatctt
      361 ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa
      421 tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa aactcacaag
      481 gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga aaataactac
      541 catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact
      601 tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac gttgattttc
      661 caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa ccagataaaa
      721 atgaatggcg acaaaatacc aacaaccatt acatcagatt cctacctaca taacggacta
      781 agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt tgaggcaaaa
      841 tttttgagtg acatgcaaag taagtatgat ctcaatggtt cgttctcatg gctcacgcaa
      901 aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg aggttcttat
      961 ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa caactgttca
     1021 ccgttacata tcaaagggaa aactgtccat atgcacagat gaaaacggtg taaaaaagat
     1081 agatacatca gagcttttac gagtttttgg tgcatttaaa gctgttcacc atgaacagat
     1141 cgacaatgta acagatgaac agcatgtaac acctaataga acaggtgaaa ccagtaaaac
     1201 aaagcaacta gaacatgaaa ttgaacacct gagacaactt gttacagctc aacagtcaca
     1261 catagacagc ctgaaacagg cgatgctgct tatcgaatca aagctgccga caacacggga
     1321 gccagtgacg cctcccgtgg ggaaaaaatc atggcggccg gcctacggcc agcctcgcag
     1381 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc
     1441 gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg
     1501 aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac
     1561 cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaagg acaattgtct
     1621 tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga
     1681 tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt
     1741 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc
     1801 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt
     1861 cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc
     1921 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag
     1981 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct
     2041 ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc
     2101 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg
     2161 cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct
     2221 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt
     2281 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg
     2341 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc
     2401 gcttccctca tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     2461 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     2521 ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     2581 ttaccaccgc tgcgttcggt caaggttcat ttaaatacgt aaatcgtaat tattggggac
     2641 ccctggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     2701 atggagttct gaggtcatta ctggatctat caacaggagt ccaagactag tttaaaccgc
     2761 ctgttttaaa actttatcga aatggccatc cattcttgcg cggatggcct ctgccagctg
     2821 ctcatagcgg ctgcgcagcg gtgagccagg acgataaacc aggccaatag tgcggcgtgg
     2881 ttccggctta atgcacggca gataaacaac cccatcgcgt ttgcgctccg gcggcacagc
     2941 cagcgctggc agtaaagtga tcccgctacc tgccgccacc atgttgcgca gagtttccag
     3001 gctggtcgcg cggaagtgtg tatcttcatc cgccccggct tcaaaacaga aacccattgc
     3061 ctgatcgcgc aaacagtgac catcttccag catcagcagt ttttcccctg ccagatcggc
     3121 catcggtacg cattcgcggt tcgcccacgg gtgatcttca tagatagcca gcaacattgg
     3181 ctcatcaaac aacggcactt caatgaatgc ttcgctctct ttcaccagcg cgaggatcac
     3241 gcaatcgagt ttgccgctgt ccagttgcgc cagtaactgg tgggtctgtg cttcatgcag
     3301 atacatttcc agctttggaa aggtctggtg cagcataggg ataatatgcg gtagcaggta
     3361 cggtccaact gtgggaatca aaccaatgtg cagcggtccg gacatcgtct cgccctgctg
     3421 gcttgccatc tctttaagga ctttcacctc acgcagcacg gtacgcgcct gatccaccag
     3481 cagcattccc gcctgggtga acaacacttt acggctggtc cgctccagca acatcacgcc
     3541 cagctcatct tccagcttac gaatttgccc gctaagcgtc ggctggctaa cgtggcagga
     3601 atctgccgca cgccgaaaat ggcggtgttc agccaatgcc accaggtact caagatcacg
     3661 aatattcatt atccatcctc catcgccacg atagttcatg gcgataggta gaatagcaat
     3721 gaacgattat ccctatcaag cattctgact gataattgct cacagcagaa ttctttgttt
     3781 aactttaatg aggaggtata cgcatgcata gcaagggcga agaagataac atggccatca
     3841 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     3901 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     3961 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     4021 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     4081 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     4141 cccaggactc ctccttgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     4201 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     4261 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     4321 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     4381 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     4441 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     4501 agctgtacaa gaggccttaa ttaattaaag gcatcaaata aaacgaaagg ctcagtcgaa
     4561 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     4621 tccgccgccc tagacagctg ggcgcgccgt atcaacaggg acaccaggat ttatttattc
     4681 tgcgaagtga tcttccgtca caggtattta ttcggcgcct gtagtgccat ttacccccat
     4741 tcactgccag agccgtgagc gcagcgaact gaatgtcacg aaaaagacag cgactcaggt
     4801 gcctgatggt cggagacaaa aggaatattc agcgatttgc ccgagcttgc gagggtgcta
     4861 cttaagcctt tagggtttta aggtctgttt tgtagaggag caaacagcgt ttgcgacatc
     4921 cttttgtaat actgcggaac tgactaaagt agtgagttat acacagggct gggatctatt
     4981 ctttttatct ttttttattc tttctttatt ctataaatta taaccacttg aatataaaca
     5041 aaaaaaacac acaaaggtct agcggaattt acagagggtc tagcagaatt tacaagtttt
     5101 ccagcaaagg tctagcagaa tttacagata cccacaactc aaaggaaaag gactagtaat
     5161 tatcattgac tagcccatct caattggtat agtgattaaa atcacctaga ccaattgaga
     5221 tgt
//
