LOCUS       sequence-384145-        8967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-HA tagged human NAP1 with S318A point mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Paul S, Sarraf SA, Nam KH, Zavar L, DeFoor N, Biswas SR, Fritsch LE,
            Yaron TM, Johnson JL, Huntsman EM, Cantley LC, Ordureau A, Pickrell 
            AM
  TITLE     NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate 
            mitosis and cytokinesis.
  JOURNAL   J Cell Biol. 2024 Feb 5;223(2):e202303082. doi: 
            10.1083/jcb.202303082. Epub 2023 Dec 7.
  PUBMED    38059900
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2024-02-5"; volume: "223(2):e202303082. doi"; pages: 
            " 10.1083/jcb.202303082. Epub 2023 Dec 7"
COMMENT     Sequence Label: pHAGE-N'-FLAG-HA-NAP1S318A
FEATURES             Location/Qualifiers
     source          1..8967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..40)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(194..782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(274..293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(953..1744,1745..1813))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1576..1595
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1814..1918)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1986..2004
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             2123..2356
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    2431..3019
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2537..2548
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2946..2966
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3036..3635)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3119..3139)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3616..3635
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    3647..4199
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3649..4216
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3809..3828)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4038..4055
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    4229..4253
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5430..5454)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5470..5496)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(5509..5532)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      5532..5541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5617..5641)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5638..5841)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5667..5687)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5842..6145
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6197..6314
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6611..6655)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6840..7073
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7570..7695
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7742..8375
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8567..8586)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8636..8652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8636..8652)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8649..8671)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8660..8676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8684..8690,8691..8708,8709..8714))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8729..8750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
       61 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      121 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      181 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      241 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      301 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      361 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      421 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      481 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      541 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      601 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      661 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      721 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      781 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      841 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      901 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      961 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1021 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1081 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1141 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1201 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1261 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1321 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1381 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1441 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1501 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1561 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1621 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1681 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1741 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1801 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1861 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1921 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     1981 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga atgatctagc cctttcctta
     2041 attaacccgg gcctctcact ctctgatatt catttctttg caagttataa atactgaata
     2101 ataagatgac atgaactact actgctagag attttccaca ctgactaaaa gggtctgagg
     2161 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     2221 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     2281 atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct tcgttgggag
     2341 tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc
     2401 ttgtaagtca ttggtcttaa aggatctcag gcggggaggc ggcccaaagg gagatccgac
     2461 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
     2521 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
     2581 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
     2641 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
     2701 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
     2761 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
     2821 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
     2881 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
     2941 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
     3001 tgtaatccag aggttgatta ggatccatcg atgcatcagg caccgggctt gcgggtcatg
     3061 caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc
     3121 tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc
     3181 tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg
     3241 ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg
     3301 aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg
     3361 ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc
     3421 gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag
     3481 agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg
     3541 gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg
     3601 gtggcgaggc gcaccgtggg cttgtactcg gtcatggttg tggccatatt atcatcgtgt
     3661 ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct agacgttttt ttaacctcga
     3721 ctaaacacat gtaaagcatg tgcaccgagg ccccagatca gatcccatac aatggggtac
     3781 cttctgggca tccttcagcc ccttgttgaa tacgcttgag gagagccatt tgactctttc
     3841 cacaactatc caactcacaa cgtggcactg gggttgtgcc gcctttgcag gtgtatctta
     3901 tacacgtggc ttttggccgc agaggcacct gtcgccaggt ggggggttcc gctgcctgca
     3961 aagggtcgct acagacgttg tttgtcttca agaagcttcc agaggaactg cttccttcac
     4021 gacattcaac agaccttgca ttcctttggc gagaggggaa agacccctag gaatgctcgt
     4081 caagaagaca gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga
     4141 aaataacata tagacaaacg cacaccggcc ttattccaag cggcttcggc cagtaacgtt
     4201 aggggggggg ggcggaattc gttatcgaac cactttgtac aagaaagttg gttaattctt
     4261 ataaaggcag ttctgattat gttgatccaa gtaatgcagt ggtggaaggt tgggtaaagg
     4321 caaagtttta gttttagttt ccccaaatgc tgtctcactt gatttaggag gacttggaaa
     4381 tacccaggaa ttgtcttcca gagaatttct gccataagaa ctgtgttcct gaaagcatgt
     4441 accatcatta ggaatggatc tctcattgtc tgtccatgct tggaggattg ctttctctga
     4501 taaaaccttt acatctcctg gtaaagggga agatgtggta tgacaaagag ctttgccatt
     4561 tggtaagaga gggggatgtc ttgtgtatgt tgcagtaaaa ttcatcaagt gcagttttgt
     4621 gctgtctctt ccaaggtctt cactgcatcc tacaggggca caggctttct tgattgcagt
     4681 tgaggttttc agttttctta gtagttcagc ttgtacttga gtcaccagat gtaaattaga
     4741 catttctctc ttcagttccc agtatgcatg ctgcatatta tcacttgaaa tgcttagttt
     4801 ttggagatct ctgctcttca gattgtcttc ctgatatgga tccgtttgtt tggctttctg
     4861 tagttctatt ttgagatcgc tacattcttt cctcatcagt tccagctctt gttccaaacc
     4921 atggatcttc aggtcacagc tcaacttttc cacctcccag tttgatgaag gtggatttaa
     4981 attcctcatc acctgctgag tttccacctc tgtcctcagc tggaggagtt ctacttcttt
     5041 agattgtagc tgctcattca atactttcaa agattcagag ttgtctttat tcattttgtc
     5101 cagtttgctc ttcaaattat ctctatcaat gcaaacctct cgatatgcat gataggcctt
     5161 atttacttgt tctcgtccca cggaacttgt ttcttcttca aatcgagcta ttagcttttc
     5221 ttccaaaaat cttattctct tctttaacaa agagttctct ttctctgaat ccttaagtcg
     5281 ttttttgatg tcttcatatg cagtgacaag agcaaaatgg gaagcaacag attcatctcc
     5341 tgaatatatt gaaactggag tcactgtatc tctcttatgg gctttttcat gattcagaat
     5401 acagatatca tcttctacca gtgcatccat gccaactttt ttgtacaaac ttgtttgata
     5461 acctcgagca gcgtaatctg gaacatcgta tgggtatcct ccatcgagct tgtcatcgtc
     5521 atccttgtag tccatggtgg cagatcaacc tcgagagatc tgcggccgcc ggtgtcttct
     5581 atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaacgagc
     5641 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
     5701 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
     5761 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
     5821 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
     5881 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     5941 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     6001 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     6061 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     6121 gccatttacc gtaagttatg taacgcggaa ctccactagt ccaaactgga tctctgctgt
     6181 ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt
     6241 gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc
     6301 cttttctttt aaaattgtgg atgaatactg ccatttgtga attaattcgg cgataccgtc
     6361 gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta
     6421 ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg
     6481 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta
     6541 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa
     6601 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt
     6661 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc
     6721 tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac
     6781 tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca
     6841 ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga
     6901 tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt
     6961 tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg
     7021 ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca
     7081 ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg
     7141 gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc
     7201 ctcctccagg tctgaagatc agcggccggc cgcttgctgt gcggtggtct tacttttgtt
     7261 ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg
     7321 cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg
     7381 aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc
     7441 aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata
     7501 ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg
     7561 cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt
     7621 tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt
     7681 cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca
     7741 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     7801 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     7861 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     7921 ccagtacagg caaaaagcag ctgcttatat gcaggatctg agggctcgcc actccccagt
     7981 cccgcccagg ccacgcctcc ctggaaagtc cccagcggaa agtcccttgt agcaagctcg
     8041 atatcagcag ttcttgaagt actccggatg cagctctcgg gccacgtgat gaaatgctag
     8101 gcggctgtca aacctccact ctaacacttc tctctccggg tcatccatcc catgcaggct
     8161 cacagggtgt aacaagctgg tgttctctcc tttattggcc tcttctacct tatctggctc
     8221 aactggtact agcttgtagc accatccaaa ggtcagtgga tatctgaccc ctggccctgg
     8281 tgtgtagttc tgctaatcag ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat
     8341 atcttgtctt ctttgggagt gaattagccc ttccaactac taagtttgta gtacatattt
     8401 aacaaataca atttctttaa aatgaaaata attcagagga atcacaggtt tagagtaaat
     8461 gaaaccacag gtaattggca gtggtaatag ggtatggggt gggaagtttg ggatgatttt
     8521 ggttagcttg agttatccag ttgatccaga catgataaga tacattgatg agtttggaca
     8581 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaattcgta atcatgtcat
     8641 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8701 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8761 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca cggggagctt tttgcaaaag
     8821 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     8881 tcggcctctg cataaataaa aaaaattagt cagccatgag cttgctgcat taatgaatcg
     8941 gccaacgcgc ggggagaggc ggtttgc
//
