LOCUS       sequence-384155-        9632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct encoding the BRAF regulatory (REG) 
            domain (AA 1-435) with a C-terminal HaloTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Spencer-Smith R, Terrell EM, Insinna C, Agamasu C, Wagner ME, Ritt 
            DA, Stauffer J, Stephen AG, Morrison DK
  TITLE     RASopathy mutations provide functional insight into the BRAF 
            cysteine-rich domain and reveal the importance of autoinhibition in 
            BRAF regulation.
  JOURNAL   Mol Cell. 2022 Nov 17;82(22):4262-4276.e5. doi: 
            10.1016/j.molcel.2022.10.016. Epub 2022 Nov 7.
  PUBMED    36347258
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.10.016"; journalName: "Mol Cell"; date: 
            "2022-11-17- 17"; volume: "82"; issue: "22"; pages: "4262-4276.e5"
COMMENT     Sequence Label: CMV-BRAF-REG-Halo
FEATURES             Location/Qualifiers
     source          1..9632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(2030..2049)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2197..2217
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2228..2531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2532..2735
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2686..2706
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2732..2756
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2801..2825
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2826..2835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4131..4155)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4160..5047
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             5060..5080
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     protein_bind    complement(5103..5123)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        complement(5185..5203)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5186..5203)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5223..5240)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5229..5453
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5499..5927
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5586..5605)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5796..5817
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5936..5956)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5941..6270
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6121..6256
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6183..6202
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6337..7131
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6391..6410)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7001..7020
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7305..7426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7342..7361)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7396..7415
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7475..7491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7475..7491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7488..7510)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7499..7515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7523..7529,7530..7547,7548..7553))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7568..7589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7706..7723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7877..8465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7957..7976)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8636..9427,9428..9496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9259..9278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9497..9601)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaac tttgtataga aaagttggcc
     2221 ctgcaggcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     2281 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     2341 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     2401 atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     2461 cccagtacat gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg
     2521 ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact
     2581 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     2641 atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta
     2701 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     2761 ggagacgcca tccacgctgt tttgacctcc atagaagaca acaactttgt acaaaaaagt
     2821 tggcaccatg gcggcgctga gcggtggcgg tggtggcggc gcggagccgg gccaggctct
     2881 gttcaacggg gacatggagc ccgaggccgg cgccggcgcc ggcgccgcgg cctcttcggc
     2941 tgcggaccct gccattccgg aggaggtgtg gaatatcaaa caaatgatta agttgacaca
     3001 ggaacatata gaggccctat tggacaaatt tggtggggag cataatccac catcaatata
     3061 tctggaggcc tatgaagaat acaccagcaa gctagatgca ctccaacaaa gagaacaaca
     3121 gttattggaa tctctgggga acggaactga tttttctgtt tctagctctg catcaatgga
     3181 taccgttaca tcttcttcct cttctagcct ttcagtgcta ccttcatctc tttcagtttt
     3241 tcaaaatccc acagatgtgg cacggagcaa ccccaagtca ccacaaaaac ctatcgttag
     3301 agtcttcctg cccaacaaac agaggacagt ggtacctgca aggtgtggag ttacagtccg
     3361 agacagtcta aagaaagcac tgatgatgag aggtctaatc ccagagtgct gtgctgttta
     3421 cagaatccag gatggagaga agaaaccaat tggttgggac actgatattt cctggcttac
     3481 tggagaagaa ttgcatgtgg aagtgttgga gaatgttcca cttacaacac acaactttgt
     3541 acgaaaaacg tttttcacct tagcattttg tgacttttgt cgaaagctgc ttttccaggg
     3601 tttccgctgt caaacatgtg gttataaatt tcaccagcgt tgtagtacag aagttccact
     3661 gatgtgtgtt aattatgacc aacttgattt gctgtttgtc tccaagttct ttgaacacca
     3721 cccaatacca caggaagagg cgtccttagc agagactgcc ctaacatctg gatcatcccc
     3781 ttccgcaccc gcctcggact ctattgggcc ccaaattctc accagtccgt ctccttcaaa
     3841 atccattcca attccacagc ccttccgacc agcagatgaa gatcatcgaa atcaatttgg
     3901 gcaacgagac cgatcctcat cagctcccaa tgtgcatata aacacaatag aacctgtcaa
     3961 tattgatgac ttgattagag accaaggatt tcgtggtgat ggaggatcaa ccacaggttt
     4021 gtctgctacc ccccctgcct cattacctgg ctcactaact aacgtgaaag ccttacagaa
     4081 atctccagga cctcagcgag aaaggaagtc atcttcatcc tcagaagaca ggtcaacttt
     4141 cttgtacaaa gtgggcggcg cagaaatcgg tactggcttt ccattcgacc cccattatgt
     4201 ggaagtcctg ggcgagcgca tgcactacgt cgatgttggt ccgcgcgatg gcacccctgt
     4261 gctgttcctg cacggtaacc cgacctcctc ctacgtgtgg cgcaacatca tcccgcatgt
     4321 tgcaccgacc catcgctgca ttgctccaga cctgatcggt atgggcaaat ccgacaaacc
     4381 agacctgggt tatttcttcg acgaccacgt ccgcttcatg gatgccttca tcgaagccct
     4441 gggtctggaa gaggtcgtcc tggtcattca cgactggggc tccgctctgg gtttccactg
     4501 ggccaagcgc aatccagagc gcgtcaaagg tattgcattt atggagttca tccgccctat
     4561 cccgacctgg gacgaatggc cagaatttgc ccgcgagacc ttccaggcct tccgcaccac
     4621 cgacgtcggc cgcaagctga tcatcgatca gaacgttttt atcgagggta cgctgccgat
     4681 gggtgtcgtc cgcccgctga ctgaagtcga gatggaccat taccgcgagc cgttcctgaa
     4741 tcctgttgac cgcgagccac tgtggcgctt cccaaacgag ctgccaatcg ccggtgagcc
     4801 agcgaacatc gtcgcgctgg tcgaagaata catggactgg ctgcaccagt cccctgtccc
     4861 gaagctgctg ttctggggca ccccaggcgt tctgatccca ccggccgaag ccgctcgcct
     4921 ggccaaaagc ctgcctaact gcaaggctgt ggacatcggc ccgggtctga atctgctgca
     4981 agaagacaac ccggacctga tcggcagcga gatcgcgcgc tggctgtcga cgctcgagat
     5041 ttccggcgag ccaaccactg aggatctgta ctttcagagc gataacgcga tcgcctaata
     5101 atcaacttta ttatacatag ttgataattc tgcagatatc catcacactg gcggccgctc
     5161 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     5221 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     5281 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5341 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5401 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5461 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     5521 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5581 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5641 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5701 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5761 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5821 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5881 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     5941 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6001 atgcatctca attagtcagc aaccaggtgt ggaaagtccc taggctcccc agcaggcaga
     6061 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6121 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6181 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     6241 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     6301 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     6361 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     6421 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     6481 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     6541 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     6601 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     6661 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     6721 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     6781 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     6841 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     6901 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     6961 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     7021 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     7081 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     7141 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     7201 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7261 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     7321 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7381 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7441 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7501 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7561 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7621 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7681 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7741 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7801 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7861 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7921 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7981 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8041 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8101 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8161 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8221 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8281 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8341 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8401 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8461 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8521 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8581 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8641 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8701 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8761 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8821 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8881 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8941 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9001 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9061 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9121 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9181 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9241 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9301 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9361 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9421 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9481 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9541 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9601 gcacatttcc ccgaaaagtg ccacctgacg tc
//
