LOCUS       sequence-384164-       11880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila Gal4 expression under introduced sequence control, PhiC31
            transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11880)
  TITLE     McCabe lab pBID2 plasmids 2021
REFERENCE   2  (bases 1 to 11880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBID2_MCS>Gal4
FEATURES             Location/Qualifiers
     source          1..11880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4792..4825
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4838..5268
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     intron          5504..5570
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             5607..8252
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    8940..9370
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    9404..9473
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(9692..9710)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9731..9747)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9731..9747)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9744..9766)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9755..9771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9779..9785,9786..9803,9804..9809))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9824..9845
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9962..9979)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10133..10721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10213..10232)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10892..11683,11684..11752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11515..11534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11753..11857)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cataacttcg
     4801 tataatgtat gctatacgaa gttatgctag cggatcctgg ccacgtaata agtgtgcgtt
     4861 gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta
     4921 tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg
     4981 tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa
     5041 acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa
     5101 atacattgca taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg
     5161 ttgcataccc aataaaagat tattatattg catacctttt cttgccatac catttagccg
     5221 atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa ccaacaacag atctgaattc
     5281 gcttaattaa ggcgcgccgc acatttaaat ctcgaggata aacggccggc cgagctcgcc
     5341 cggggatcga gcgcagcggt ataaaagggc gcggggtggc tgagagcatc agttgtgaat
     5401 gaatgttcga gccgagcaga cgtgccgctg ccttcgttaa tatcctttga ataagccaac
     5461 tttgaatcac aagacgcata ccaaacggta ccagatctaa aaggtaggtt caaccactga
     5521 tgcctaggca caccgaaacg actaacccta attcttatcc tttacttcag gcggccgcgt
     5581 taaacaactt aaaaaaaaaa atcaaaatga agctgctgag tagtattgaa caagcgtgtg
     5641 acatatgccg acttaagaag ctgaagtgca gcaaggaaaa gccgaagtgc gccaaatgtc
     5701 ttaagaacaa ttgggagtgc cggtactcgc caaagacgaa gcgaagccct ttgacacgcg
     5761 ctcatttgac tgaagtggag agtcgcctgg agcgccttga gcagttgttc ctcctcattt
     5821 ttccccgtga agacctcgac atgatactga agatggatag tctccaagat attaaggccc
     5881 tgctcactgg cctgtttgtt caggataacg tcaacaagga tgccgtgacg gaccgccttg
     5941 cctcggtgga gacagatatg ccacttacct tgcggcagca tcgcatctcg gctaccagca
     6001 gtagtgaaga aagttccaat aagggtcaac ggcaactgac ggtatcgatc gacagtgcgg
     6061 cccaccacga caacagcacc attccactgg acttcatgcc tcgtgacgca ctgcatggat
     6121 ttgactggag cgaggaggat gatatgagcg acggactccc attcctgaag accgacccca
     6181 ataacaatgg tttctttggc gacggcagcc tgctgtgtat cctgcgaagt attggtttta
     6241 agccggaaaa ttacacgaac agtaatgtca atcgtcttcc cacaatgatc accgaccgtt
     6301 acacgctggc cagtcgcagt actacaagcc gcctgctgca aagctatctt aacaacttcc
     6361 acccctactg ccccatagtg catagtccta ctctcatgat gctttacaac aatcagattg
     6421 agatagcatc gaaggaccag tggcagatcc tcttcaactg cattctcgcc atcggcgctt
     6481 ggtgtattga aggcgagagc acagatatcg acgtgtttta ctatcaaaac gccaagtcgc
     6541 acctcacctc gaaggtcttc gaaagtggaa gcattatcct cgtgacagcc ctccatctgc
     6601 tgagtcgtta tacccagtgg cgtcagaaaa ctaatacatc ctacaacttt cattcgttca
     6661 gcattcggat ggcgattagc ctgggactta atcgtgacct cccaagtagc ttcagcgatt
     6721 cgtcgatcct ggaacagcgc cggaggattt ggtggagtgt gtacagctgg gagatccagc
     6781 tgtccctctt gtacggacgc agcattcagc tgagtcagaa cacgatttcc ttcccgtcca
     6841 gcgtggacga cgttcaacgg acaactacgg gcccgaccat ctaccacggt atcattgaga
     6901 cggcccgctt gcttcaagtg tttacgaaga tttatgagct ggataagaca gtgaccgcgg
     6961 agaagagtcc gatctgcgcc aagaaatgcc tgatgatctg caatgagata gaggaggtga
     7021 gccgccaggc acccaagttc ctgcagatgg acataagcac tacggcgctg actaatctcc
     7081 tcaaagaaca cccctggctg agtttcaccc gcttcgagct gaagtggaaa cagctgagtc
     7141 tgattatata cgttttgcgg gactttttca caaattttac gcagaagaag tcgcagcttg
     7201 agcaagatca gaacgaccat cagtcctacg aggtaaagag gtgcagcata atgctctccg
     7261 acgcagccca gcgcaccgtg atgtcggtgt cgtcctatat ggacaaccat aatgtgaccc
     7321 cttatttcgc ctggaattgc tcctactatt tgttcaacgc cgtgttggtc ccaatcaaga
     7381 ctcttctgag caactccaaa tcgaacgccg agaataacga gacagcacag ctcttgcagc
     7441 aaattaacac cgtgttgatg ctgttgaaga aactcgctac attcaagatt cagacttgcg
     7501 aaaagtacat ccaggtgttg gaagaggtgt gcgccccatt ccttctttcg caatgcgcaa
     7561 ttcccctccc gcacataagt tacaataatt ccaacggctc cgctatcaag aatatcgtcg
     7621 gctccgctac aatcgcgcag tatcccacgc tgcctgagga aaatgttaac aacatatcgg
     7681 tgaagtatgt aagtccaggc agcgtcggcc cgagccctgt gccgctgaaa agcggagcct
     7741 ccttcagcga tctggtcaag ctgctcagca accgcccacc atcccgcaac tcccctgtca
     7801 ctataccccg atcgaccccg tcgcatcgta gcgttacccc attcctggga caacagcagc
     7861 aactgcagag tctggtgcca cttacgccct cggcattgtt tggcggagcc aacttcaacc
     7921 agagtggtaa cattgcggac agctccttga gcttcacgtt taccaacagc tccaacggtc
     7981 caaacttgat cacgactcag acaaactccc aggcattgtc ccagcccatt gccagctcga
     8041 atgtgcatga taactttatg aataatgaaa ttactgcctc caagattgac gacggcaaca
     8101 acagcaagcc cctttcccca ggctggactg accagacggc ctacaatgct ttcggtatca
     8161 ccactggcat gttcaacacc acaacgatgg acgacgtata taactatctt tttgatgatg
     8221 aggatactcc accgaaccca aagaaggagt agcctgcagg aatgaatcgt ttttaaaata
     8281 acaaatcaat tgttttataa tattcgtacg attctttgat tatgtaataa aatgtgatca
     8341 ttaggaagat tacgaaaaat ataaaaaata tgagttctgt gtgtataaca aatgctgtaa
     8401 acgccacaat tgtgtttgtt gcaaataaac ccatgattat ttgattaaaa ttgttgtttt
     8461 ctttgttcat agacaatagt gtgttttgcc taaacgtgta ctgcataaac tccatgcgag
     8521 tgtatagcga gctagtggct aacgcttgcc ccaccaaagt agattcgtca aaatcctcaa
     8581 tttcatcacc ctcctccaag tttaacattt ggccgtcgga attaacttct aaagatgcca
     8641 cataatctaa taaatgaaat agagattcaa acgtggcgtc atcgtccgtt tcgaccattt
     8701 ccgaaaagaa ctcgggcata aactctatga tttctctgga cgtggtgttg tcgaaactct
     8761 caaagtacgc agtcaggaac gtgcgcgaca tgtcgtcggg aaactcgcgc ggaaacatgt
     8821 tgttgtaacc gaacgggtcc catagcgcca aaaccaaatc tgccagcgtc aatagaatga
     8881 gcacgatgcc gacaatggag ctggcttgga tagcgattcg agttaactct agaagatctt
     8941 ggccacgtaa taagtgtgcg ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag
     9001 taaaattttg ttgcatacct tatcaaaaaa taagtgctgc atacttttta gagaaaccaa
     9061 ataatttttt attgcatacc cgtttttaat aaaatacatt gcataccctc ttttaataaa
     9121 aaatattgca tactttgacg aaacaaattt tcgttgcata cccaataaaa gattattata
     9181 ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa aatattgcat
     9241 acgttgacga aacaaatttt cgttgcatac ccaataaaag attattatat tgcatacctt
     9301 ttcttgccat accatttagc cgatcaattg tgctcggcaa cagtatattt gtggtgtgcc
     9361 aaccaacaac ggatccacta gtgtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg
     9421 ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc atctggtcca
     9481 tcatgatgaa cgggtcgagg tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga
     9541 agccctcgcc ctcgaaaccg ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg
     9601 cgtcggcggg tgcggatacg cggggcagcg tcagcgggtt ctcgacggtc acggcgggca
     9661 tgtcgacact agttctagcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg
     9721 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     9781 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     9841 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9901 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9961 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10021 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10081 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10141 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10201 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10261 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10321 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10381 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10441 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10501 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10561 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10621 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10681 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10741 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10801 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10861 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10921 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10981 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    11041 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    11101 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    11161 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    11221 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    11281 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    11341 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    11401 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    11461 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11521 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11581 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11641 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11701 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11761 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11821 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
