LOCUS       sequence-384166-        8743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses guide RNA for targeting MAK10 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8743)
  TITLE     Yeast viruses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAS-mak10 gRNA
FEATURES             Location/Qualifiers
     source          1..8743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(36..624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(116..135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      646..1628
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(1609..1656)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_RNA        2029..2104
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gene            2238..3594
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        2238..2581
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2582..3391
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2649..2668)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      3397..3594
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             4357..8460
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4357..8460
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8470..8490
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8506..8529
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      8533..8722
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
       61 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      121 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      181 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      241 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      301 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      361 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      421 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      481 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      541 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      601 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctaccga agcatctgtg
      661 cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
      721 agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc
      781 tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
      841 atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa
      901 gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca
      961 aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac
     1021 tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt
     1081 ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg
     1141 cattttttca agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac
     1201 tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt
     1261 ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt
     1321 cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga
     1381 taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg
     1441 ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg
     1501 tttgtggaag cggtattcgc aatattttag tagcccgtta cagtccggtg cgtttttggt
     1561 tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata
     1621 ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct
     1681 tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct
     1741 gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa
     1801 atgcgtatat gtgttatgta gtatactctt tcttcaacaa ttaaatactc tcggtagcca
     1861 agttggttta aggcgcaaga ctgtaattta tcactacgaa atcttgagat cgggcgttcg
     1921 actcgccccc gggagagatg gccggcatgg tcccagcctc ctcgctggcg ccggctgggc
     1981 aacaccttcg ggtggcgaat gggacttttt tcaattagct catccagggt tttagagcta
     2041 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     2101 gtgctttttt tattttttgt cactattgtt atgtaaaatg ccacctctga cagtatggaa
     2161 cgcaaacttc tgtctagtgg atataggtct agagatctgt ttagcttgcc tcgtccccgc
     2221 cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc
     2281 gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat
     2341 aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct
     2401 cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg
     2461 ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata
     2521 tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac
     2581 catgggtaag gaaaagactc acgtttcgag gccgcgatta aattccaaca tggatgctga
     2641 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     2701 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     2761 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     2821 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     2881 cggcaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     2941 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     3001 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     3061 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     3121 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     3181 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     3241 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     3301 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     3361 gtttcatttg atgctcgatg agtttttcta atcagtactg acaataaaaa gattcttgtt
     3421 ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat
     3481 gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt
     3541 gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg
     3601 attcgatact aacgccgcca tccagtgtcg aaaacgagct ctcgagaacc cttaatagtc
     3661 gaacaagaag caggcaaagt ttagagcact gcccctccgc actcaaaaaa gaaaaaacta
     3721 ggaggaaaat aaaattctca accacacaaa cacataaaca catacaaata caaatacaag
     3781 cttatttact tgacatcgcg cgatcttcca ctattcagcg ccgtccgccc tctctcgtgt
     3841 tttttgttta cgcgacaact atgcgaaatc cggagcaacg ggcaaccgtt tggggaaaga
     3901 ccacacccac gcgcgatcgc catggcaacg aggtcgcaca cgccccacac ccagacctcc
     3961 ctgcgagcgg gcatgggtac aatgtccccg ttgccacaga caccacttcg tagcacagcg
     4021 cagagcgtag cgtgttgttg ctgctgacaa aagaaaattt ttcttagcaa agcaaaggag
     4081 gggaagcacg ggcagatagc accgtaccat acccttggaa actcgaaatg aacgaagcag
     4141 gaaatgagag aatgagagtt ttgtaggtat atatagcggt agtgtttgcg cgttaccatc
     4201 atcttctgga tctatctatt gttcttttcc tcatcacttt cccctttttc gctcttcttc
     4261 ttgtctttta tttctttctt ttttttaatt gttccctcga ttggctatct accaaagaat
     4321 ccaaacttaa tacacgtatt tatttgtcca attaccatgg ataagaaata ctcaataggc
     4381 ttagatatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata taaggttccg
     4441 tctaaaaagt tcaaggttct gggaaataca gaccgccaca gtatcaaaaa aaatcttata
     4501 ggggctcttt tatttgacag tggagagaca gcggaagcga ctcgtctcaa acggacagct
     4561 cgtagaaggt atacacgtcg gaagaatcgt atttgttatc tacaggagat tttttcaaat
     4621 gagatggcga aagtagatga tagtttcttt catcgacttg aagagtcttt tttggtggaa
     4681 gaagacaaga agcatgaacg tcatcctatt tttggaaata tagtagatga agttgcttat
     4741 catgagaaat atccaactat ctatcatctg cgaaaaaaat tggtagattc tactgataaa
     4801 gcggatttgc gcttaatcta tttggcctta gcgcatatga ttaagtttcg tggtcatttt
     4861 ttgattgagg gagatttaaa tcctgataat agtgatgtgg acaaactatt tatccagttg
     4921 gtacaaacct acaatcaatt atttgaagaa aaccctatta acgcaagtgg agtagatgct
     4981 aaagcgattc tttctgcacg attgagtaaa tcaagacgat tagaaaatct cattgctcag
     5041 ctccccggtg agaagaaaaa tggcttattt gggaatctca ttgctttgtc attgggtttg
     5101 acccctaatt ttaaatcaaa ttttgatttg gcagaagatg ctaaattaca gctttcaaaa
     5161 gatacttacg atgatgattt agataattta ttggcgcaaa ttggagatca atatgctgat
     5221 ttgtttttgg cagctaagaa tttatcagat gctattttac tttcagatat cctaagagta
     5281 aatactgaaa taactaaggc tcccctatca gcttcaatga ttaaacgcta cgatgaacat
     5341 catcaagact tgactctttt aaaagcttta gttcgacaac aacttccaga aaagtataaa
     5401 gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt atattgatgg gggagctagc
     5461 caagaagaat tttataaatt tatcaaacca attttagaaa aaatggatgg tactgaggaa
     5521 ttattggtga aactaaatcg tgaagatttg ctgcgcaagc aacggacctt tgacaacggc
     5581 tctattcccc atcaaattca cttgggtgag ctgcatgcta ttttgagaag acaagaagac
     5641 ttttatccat ttttaaaaga caatcgtgag aagattgaaa aaatcttgac ttttcgaatt
     5701 ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt ttgcatggat gactcggaag
     5761 tctgaagaaa caattacccc atggaatttt gaagaagttg tcgataaagg tgcttcagct
     5821 caatcattta ttgaacgcat gacaaacttt gataaaaatc ttccaaatga aaaagtacta
     5881 ccaaaacata gtttgcttta tgagtatttt acggtttata acgaattgac aaaggtcaaa
     5941 tatgttactg aaggaatgcg aaaaccagca tttctttcag gtgaacagaa gaaagccatt
     6001 gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta agcaattaaa agaagattat
     6061 ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag gagttgaaga tagatttaat
     6121 gcttcattag gtacctacca tgatttgcta aaaattatta aagataaaga ttttttggat
     6181 aatgaagaaa atgaagatat cttagaggat attgttttaa cattgacctt atttgaagat
     6241 agggagatga ttgaggaaag acttaaaaca tatgctcacc tctttgatga taaggtgatg
     6301 aaacagctta aacgtcgccg ttatactggt tggggacgtt tgtctcgaaa attgattaat
     6361 ggtattaggg ataagcaatc tggcaaaaca atattagatt ttttgaaatc agatggtttt
     6421 gccaatcgca attttatgca gctgatccat gatgatagtt tgacatttaa agaagacatt
     6481 caaaaagcac aagtgtctgg acaaggcgat agtttacatg aacatattgc aaatttagct
     6541 ggtagccctg ctattaaaaa aggtatttta cagactgtaa aagttgttga tgaattggtc
     6601 aaagtaatgg ggcggcataa gccagaaaat atcgttattg aaatggcacg tgaaaatcag
     6661 acaactcaaa agggccagaa aaattcgcga gagcgtatga aacgaatcga agaaggtatc
     6721 aaagaattag gaagtcagat tcttaaagag catcctgttg aaaatactca attgcaaaat
     6781 gaaaagctct atctctatta tctccaaaat ggaagagaca tgtatgtgga ccaagaatta
     6841 gatattaatc gtttaagtga ttatgatgtc gatcacattg ttccacaaag tttccttaaa
     6901 gacgattcaa tagacaataa ggtcttaacg cgttctgata aaaatcgtgg taaatcggat
     6961 aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact attggagaca acttctaaac
     7021 gccaagttaa tcactcaacg taagtttgat aatttaacga aagctgaacg tggaggtttg
     7081 agtgaacttg ataaagctgg ttttatcaaa cgccaattgg ttgaaactcg ccaaatcact
     7141 aagcatgtgg cacaaatttt ggatagtcgc atgaatacta aatacgatga aaatgataaa
     7201 cttattcgag aggttaaagt gattacctta aaatctaaat tagtttctga cttccgaaaa
     7261 gatttccaat tctataaagt acgtgagatt aacaattacc atcatgccca tgatgcgtat
     7321 ctaaatgccg tcgttggaac tgctttgatt aagaaatatc caaaacttga atcggagttt
     7381 gtctatggtg attataaagt ttatgatgtt cgtaaaatga ttgctaagtc tgagcaagaa
     7441 ataggcaaag caaccgcaaa atatttcttt tactctaata tcatgaactt cttcaaaaca
     7501 gaaattacac ttgcaaatgg agagattcgc aaacgccctc taatcgaaac taatggggaa
     7561 actggagaaa ttgtctggga taaagggcga gattttgcca cagtgcgcaa agtattgtcc
     7621 atgccccaag tcaatattgt caagaaaaca gaagtacaga caggcggatt ctccaaggag
     7681 tcaattttac caaaaagaaa ttcggacaag cttattgctc gtaaaaaaga ctgggatcca
     7741 aaaaaatatg gtggttttga tagtccaacg gtagcttatt cagtcctagt ggttgctaag
     7801 gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag agttactagg gatcacaatt
     7861 atggaaagaa gttcctttga aaaaaatccg attgactttt tagaagctaa aggatataag
     7921 gaagttaaaa aagacttaat cattaaacta cctaaatata gtctttttga gttagaaaac
     7981 ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa aaggaaatga gctggctctg
     8041 ccaagcaaat atgtgaattt tttatattta gctagtcatt atgaaaagtt gaagggtagt
     8101 ccagaagata acgaacaaaa acaattgttt gtggagcagc ataagcatta tttagatgag
     8161 attattgagc aaatcagtga attttctaag cgtgttattt tagcagatgc caatttagat
     8221 aaagttctta gtgcatataa caaacataga gacaaaccaa tacgtgaaca agcagaaaat
     8281 attattcatt tatttacgtt gacgaatctt ggagctcccg ctgcttttaa atattttgat
     8341 acaacaattg atcgtaaacg atatacgtct acaaaagaag ttttagatgc cactcttatc
     8401 catcaatcca tcactggtct ttatgaaaca cgcattgatt tgagtcagct aggaggtgac
     8461 ggtggagggc caaaaaagaa aagaaaagtt gaagatgctt ctgctcatca tcaccatcac
     8521 catcaccatt aaatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     8581 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     8641 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     8701 gaaggttttg ggacgctcga agtgagcaaa aggccagcaa aag
//
