LOCUS       sequence-384173-        9680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For CRISPR-mediated deletin og MAK10 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9680)
  TITLE     Yeast viruses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAS-mak10 gRNA-MAK10-Delta
FEATURES             Location/Qualifiers
     source          1..9680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      121..709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     610..629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(766..955)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(959..982)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(998..1018)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1028..5131)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(1028..5131)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    6297..6330
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            complement(6831..8187)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(6831..7028)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(7034..7843)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7757..7776
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(7844..8187)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     misc_RNA        complement(8321..8396)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8769..8816
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      join(8797..9680,1..99)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tttctgttct gtaaaaatgc agctcagatt ctttgtttga aaaattagcg ctctcgcgtt
       61 gcatttttgt tctacaaaat gaagcacaga tgcttcggta gaaaagatca aaggatcttc
      121 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
      181 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      241 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      301 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      361 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      421 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      481 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      541 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      601 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      661 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      721 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacttcg agcgtcccaa
      781 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa
      841 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata
      901 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatttaat
      961 ggtgatggtg atggtgatga tgagcagaag catcttcaac ttttcttttc ttttttggcc
     1021 ctccaccgtc acctcctagc tgactcaaat caatgcgtgt ttcataaaga ccagtgatgg
     1081 attgatggat aagagtggca tctaaaactt cttttgtaga cgtatatcgt ttacgatcaa
     1141 ttgttgtatc aaaatattta aaagcagcgg gagctccaag attcgtcaac gtaaataaat
     1201 gaataatatt ttctgcttgt tcacgtattg gtttgtctct atgtttgtta tatgcactaa
     1261 gaactttatc taaattggca tctgctaaaa taacacgctt agaaaattca ctgatttgct
     1321 caataatctc atctaaataa tgcttatgct gctccacaaa caattgtttt tgttcgttat
     1381 cttctggact acccttcaac ttttcataat gactagctaa atataaaaaa ttcacatatt
     1441 tgcttggcag agccagctca tttccttttt gtaattctcc ggcactagcc agcatccgtt
     1501 tacgaccgtt ttctaactca aaaagactat atttaggtag tttaatgatt aagtcttttt
     1561 taacttcctt atatccttta gcttctaaaa agtcaatcgg atttttttca aaggaacttc
     1621 tttccataat tgtgatccct agtaactctt taacggattt taacttcttc gatttccctt
     1681 tttccacctt agcaaccact aggactgaat aagctaccgt tggactatca aaaccaccat
     1741 atttttttgg atcccagtct tttttacgag caataagctt gtccgaattt ctttttggta
     1801 aaattgactc cttggagaat ccgcctgtct gtacttctgt tttcttgaca atattgactt
     1861 ggggcatgga caatactttg cgcactgtgg caaaatctcg ccctttatcc cagacaattt
     1921 ctccagtttc cccattagtt tcgattagag ggcgtttgcg aatctctcca tttgcaagtg
     1981 taatttctgt tttgaagaag ttcatgatat tagagtaaaa gaaatatttt gcggttgctt
     2041 tgcctatttc ttgctcagac ttagcaatca ttttacgaac atcataaact ttataatcac
     2101 catagacaaa ctccgattca agttttggat atttcttaat caaagcagtt ccaacgacgg
     2161 catttagata cgcatcatgg gcatgatggt aattgttaat ctcacgtact ttatagaatt
     2221 ggaaatcttt tcggaagtca gaaactaatt tagattttaa ggtaatcact ttaacctctc
     2281 gaataagttt atcattttca tcgtatttag tattcatgcg actatccaaa atttgtgcca
     2341 catgcttagt gatttggcga gtttcaacca attggcgttt gataaaacca gctttatcaa
     2401 gttcactcaa acctccacgt tcagctttcg ttaaattatc aaacttacgt tgagtgatta
     2461 acttggcgtt tagaagttgt ctccaatagt ttttcatctt tttgactact tcttcacttg
     2521 gaacgttatc cgatttacca cgatttttat cagaacgcgt taagacctta ttgtctattg
     2581 aatcgtcttt aaggaaactt tgtggaacaa tgtgatcgac atcataatca cttaaacgat
     2641 taatatctaa ttcttggtcc acatacatgt ctcttccatt ttggagataa tagagataga
     2701 gcttttcatt ttgcaattga gtattttcaa caggatgctc tttaagaatc tgacttccta
     2761 attctttgat accttcttcg attcgtttca tacgctctcg cgaatttttc tggccctttt
     2821 gagttgtctg attttcacgt gccatttcaa taacgatatt ttctggctta tgccgcccca
     2881 ttactttgac caattcatca acaactttta cagtctgtaa aatacctttt ttaatagcag
     2941 ggctaccagc taaatttgca atatgttcat gtaaactatc gccttgtcca gacacttgtg
     3001 ctttttgaat gtcttcttta aatgtcaaac tatcatcatg gatcagctgc ataaaattgc
     3061 gattggcaaa accatctgat ttcaaaaaat ctaatattgt tttgccagat tgcttatccc
     3121 taataccatt aatcaatttt cgagacaaac gtccccaacc agtataacgg cgacgtttaa
     3181 gctgtttcat caccttatca tcaaagaggt gagcatatgt tttaagtctt tcctcaatca
     3241 tctccctatc ttcaaataag gtcaatgtta aaacaatatc ctctaagata tcttcatttt
     3301 cttcattatc caaaaaatct ttatctttaa taatttttag caaatcatgg taggtaccta
     3361 atgaagcatt aaatctatct tcaactcctg aaatttcaac actatcaaaa cattctattt
     3421 ttttgaaata atcttctttt aattgcttaa cggttacttt tcgatttgtt ttgaagagta
     3481 aatcaacaat ggctttcttc tgttcacctg aaagaaatgc tggttttcgc attccttcag
     3541 taacatattt gacctttgtc aattcgttat aaaccgtaaa atactcataa agcaaactat
     3601 gttttggtag tactttttca tttggaagat ttttatcaaa gtttgtcatg cgttcaataa
     3661 atgattgagc tgaagcacct ttatcgacaa cttcttcaaa attccatggg gtaattgttt
     3721 cttcagactt ccgagtcatc catgcaaaac gactattgcc acgcgccaat ggaccaacat
     3781 aataaggaat tcgaaaagtc aagatttttt caatcttctc acgattgtct tttaaaaatg
     3841 gataaaagtc ttcttgtctt ctcaaaatag catgcagctc acccaagtga atttgatggg
     3901 gaatagagcc gttgtcaaag gtccgttgct tgcgcagcaa atcttcacga tttagtttca
     3961 ccaataattc ctcagtacca tccatttttt ctaaaattgg tttgataaat ttataaaatt
     4021 cttcttggct agctccccca tcaatataac ctgcatatcc gttttttgat tgatcaaaaa
     4081 agatttcttt atacttttct ggaagttgtt gtcgaactaa agcttttaaa agagtcaagt
     4141 cttgatgatg ttcatcgtag cgtttaatca ttgaagctga taggggagcc ttagttattt
     4201 cagtatttac tcttaggata tctgaaagta aaatagcatc tgataaattc ttagctgcca
     4261 aaaacaaatc agcatattga tctccaattt gcgccaataa attatctaaa tcatcatcgt
     4321 aagtatcttt tgaaagctgt aatttagcat cttctgccaa atcaaaattt gatttaaaat
     4381 taggggtcaa acccaatgac aaagcaatga gattcccaaa taagccattt ttcttctcac
     4441 cggggagctg agcaatgaga ttttctaatc gtcttgattt actcaatcgt gcagaaagaa
     4501 tcgctttagc atctactcca cttgcgttaa tagggttttc ttcaaataat tgattgtagg
     4561 tttgtaccaa ctggataaat agtttgtcca catcactatt atcaggattt aaatctccct
     4621 caatcaaaaa atgaccacga aacttaatca tatgcgctaa ggccaaatag attaagcgca
     4681 aatccgcttt atcagtagaa tctaccaatt tttttcgcag atgatagata gttggatatt
     4741 tctcatgata agcaacttca tctactatat ttccaaaaat aggatgacgt tcatgcttct
     4801 tgtcttcttc caccaaaaaa gactcttcaa gtcgatgaaa gaaactatca tctactttcg
     4861 ccatctcatt tgaaaaaatc tcctgtagat aacaaatacg attcttccga cgtgtatacc
     4921 ttctacgagc tgtccgtttg agacgagtcg cttccgctgt ctctccactg tcaaataaaa
     4981 gagcccctat aagatttttt ttgatactgt ggcggtctgt atttcccaga accttgaact
     5041 ttttagacgg aaccttatat tcatcagtga tcaccgccca tccgacgcta tttgtgccga
     5101 tatctaagcc tattgagtat ttcttatcca tggtaattgg acaaataaat acgtgtatta
     5161 agtttggatt ctttggtaga tagccaatcg agggaacaat taaaaaaaag aaagaaataa
     5221 aagacaagaa gaagagcgaa aaaggggaaa gtgatgagga aaagaacaat agatagatcc
     5281 agaagatgat ggtaacgcgc aaacactacc gctatatata cctacaaaac tctcattctc
     5341 tcatttcctg cttcgttcat ttcgagtttc caagggtatg gtacggtgct atctgcccgt
     5401 gcttcccctc ctttgctttg ctaagaaaaa ttttcttttg tcagcagcaa caacacgcta
     5461 cgctctgcgc tgtgctacga agtggtgtct gtggcaacgg ggacattgta cccatgcccg
     5521 ctcgcaggga ggtctgggtg tggggcgtgt gcgacctcgt tgccatggcg atcgcgcgtg
     5581 ggtgtggtct ttccccaaac ggttgcccgt tgctccggat ttcgcatagt tgtcgcgtaa
     5641 acaaaaaaca cgagagaggg cggacggcgc tgaatagtgg aagatcgcgc gatgtcaagt
     5701 aaataagctt gtatttgtat ttgtatgtgt ttatgtgttt gtgtggttga gaattttatt
     5761 ttcctcctag ttttttcttt tttgagtgcg gaggggcagt gctctaaact ttgcctgctt
     5821 cttgttcgac tattaagggt tctcgagcac ccgcaaatca gaagaaagga acttctccct
     5881 cccactttaa agtaaatccg gagattcccg ttattacctt acatatcgaa atatgaccca
     5941 taaaagatgt gtaaaattac gtaagacaaa ggaactcggc acacctattg aataggtaat
     6001 agtggaatca ggtgcctaaa taaatgaata agagtactgt attatctagt agttgttata
     6061 ttatctaatc tttacataat acgattattt cacattgtta tgttttcggg taataattat
     6121 gagatggagt gaaatttcac gaagagatac aagtaaaata gcagctcttc aatagcaaga
     6181 agagtggcgc tgagattgaa aacaactgtg ctagtgtaga gcaactttga taatagctgg
     6241 tacgtttccg agaatgcagc tgaagcttcg tacgctgcag gtcgacaacc cttaatataa
     6301 cttcgtataa tgtatgctat acgaagttat taggtgatat cagatccact agtggcctat
     6361 aatctggaat ttaccctaac tgtacaaaat atatttagca ttatataccc gctatttaat
     6421 tattataaag gagaccttta tactcaaaca aatgaagcca ccttatgcca ttatcccatt
     6481 cgtaccagtc taaaacaaga taaataccct gtttaacatc cgaacaccaa atatgtgtgg
     6541 gtgtcaaaca aagagacaat gtaatgaggc aatgtactgc ttgtaaacta taacagtatc
     6601 agctcaattt caatgccatc tactccagat atcgttgcga ttgtacctac aagccgccta
     6661 caacggcgtt attgttgtaa gctggtaaaa tgcagtgcgt tcccccgttg tgagttcggg
     6721 ccaaacttac ttggctggga gttccgtccg tacagttgtg gcttgctgcc tagacttact
     6781 cgagagctcg ttttcgacac tggatggcgg cgttagtatc gaatcgacag cagtatagcg
     6841 accagcattc acatacgatt gacgcatgat attactttct gcgcacttaa cttcgcatct
     6901 gggcagatga tgtcgaggcg aaaaaaaata taaatcacgc taacatttga ttaaaataga
     6961 acaactacaa tataaaaaaa ctatacaaat gacaagttct tgaaaacaag aatcttttta
     7021 ttgtcagtac tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     7081 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     7141 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     7201 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     7261 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     7321 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     7381 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     7441 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     7501 ctgaatcagg atattcttct aatacctgga atgctgtttt gccggggatc gcagtggtga
     7561 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     7621 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     7681 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     7741 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     7801 aatttaatcg cggcctcgaa acgtgagtct tttccttacc catggttgtt tatgttcgga
     7861 tgtgatgtga gaactgtatc ctagcaagat tttaaaagga agtatatgaa agaagaacct
     7921 cagtggcaaa tcctaacctt ttatatttct ctacaggggc gcggcgtggg gacaattcaa
     7981 cgcgtctgtg aggggagcgt ttccctgctc gcaggtctgc agcgaggagc cgtaattttt
     8041 gcttcgcgcc gtgcggccat caaaatgtat ggatgcaaat gattatacat ggggatgtat
     8101 gggctaaatg tacgggcgac agtcacatca tgcccctgag ctgcgcacgt caagactgtc
     8161 aaggagggta ttctgggcct ccatgtcgct ggccgggtga cccggcgggg acgaggcaag
     8221 ctaaacagat ctctagacct atatccacta gacagaagtt tgcgttccat actgtcagag
     8281 gtggcatttt acataacaat agtgacaaaa aataaaaaaa gcaccgactc ggtgccactt
     8341 tttcaagttg ataacggact agccttattt taacttgcta tttctagctc taaaaccctg
     8401 gatgagctaa ttgaaaaaag tcccattcgc cacccgaagg tgttgcccag ccggcgccag
     8461 cgaggaggct gggaccatgc cggccatctc tcccgggggc gagtcgaacg cccgatctca
     8521 agatttcgta gtgataaatt acagtcttgc gccttaaacc aacttggcta ccgagagtat
     8581 ttaattgttg aagaaagagt atactacata acacatatac gcatttaagc ataaacacgc
     8641 actatgccgt tcttctcatg tatatatata tacaggcaac acgcagatat aggtgcgacg
     8701 tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt cggaagcgct cgttttcgga
     8761 aacgctttga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcagag
     8821 cgcttttgaa aaccaaaagc gctctgaaga cgcactttca aaaaaccaaa aacgcaccgg
     8881 actgtaacgg gctactaaaa tattgcgaat accgcttcca caaacattgc tcaaaagtat
     8941 ctctttgcta tatatctctg tgctatatcc ctatataacc tacccatcca cctttcgctc
     9001 cttgaacttg catctaaact cgacctctac attttttatg tttatctcta gtattactct
     9061 ttagacaaaa aaattgtagt aagaactatt catagagtga atcgaaaaca atacgaaaat
     9121 gtaaacattt cctatacgta gtatatagag acaaaataga agaaaccgtt cataattttc
     9181 tgaccaatga agaatcatca acgctatcac tttctgttca caaagtatgc gcaatccaca
     9241 tcggtataga atataatcgg ggatgccttt atcttgaaaa aatgcacccg cagcttcgct
     9301 agtaatcagt aaacgcggga agtggagtca ggcttttttt atggaagaga aaatagacac
     9361 caaagtagcc ttcttctaac cttaacggac ctacagtgca aaaagttatc aagagactgc
     9421 attatagagc gcacaaagga gaaaaaaagt aatctaagat gctttgttag aaaaatagcg
     9481 ctctcgggat gcatttttgt agaacaaaaa agaagtatag attctttgtt ggtaaaatag
     9541 cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg tttgaaaaat
     9601 tagcgctctc gcgttgcatt tttgttttac aaaaatgaag cacagattct tcgttggtaa
     9661 aatagcgctt tcgcgttgca
//
