LOCUS       sequence-384230-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_T1027I in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_T1027I
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(39..830,831..899))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     662..681
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(900..1004)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1079..1098)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1270..1649
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1650..1853
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1804..1824
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1855..1873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1876..1894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1904..1928
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2071..2095
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5915..5939)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5986..6009
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6046..6069
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6100..6126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6100..6126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(6127..6474,6475..6477,6478..7086)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(7130..7147)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7136..7360
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7493..7512)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7644..7691
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7699..8721
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7699..8721
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8851..8972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8888..8907)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8942..8961
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9021..9037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9021..9037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9034..9056)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9045..9061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9069..9075,9076..9093,9094..9099))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9114..9135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9252..9269)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9423..10011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9503..9522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
       61 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
      121 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
      181 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
      241 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
      301 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
      361 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
      421 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
      481 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
      541 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
      601 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
      661 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
      721 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
      781 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
      841 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
      901 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
      961 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     1021 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     1081 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     1141 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     1201 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     1261 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     1321 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     1381 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     1441 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     1501 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     1561 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1621 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     1681 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     1741 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     1801 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta
     1861 tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc
     1921 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     1981 gatccagcct ccggactcta gcgtttaaac ttaagcttgg taccgagctc ggatccacta
     2041 gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagt tggcatgttc
     2101 gtgttcctgg tgctgctgcc actggtgagc tcccagtgcg tgaacctgac caccagaacc
     2161 cagctgccac ctgcctacac caactccttc accaggggcg tgtactaccc agacaaggtg
     2221 ttcagatcca gcgtgctgca ctctacccag gacctgttcc tgccattctt ctctaacgtg
     2281 acctggttcc acgccatcca cgtgtctgga accaacggca ccaagagatt cgacaaccca
     2341 gtgctgccat tcaacgacgg cgtgtacttc gcttctaccg agaagtctaa catcatcagg
     2401 ggttggatct tcggaaccac cctggactct aagacccagt ctctgctgat cgtgaacaac
     2461 gctaccaacg tggtgatcaa ggtgtgcgag ttccagttct gcaacgaccc attcctggga
     2521 gtgtactacc acaagaacaa caagtcttgg atggaaagcg agttcagggt gtacagctct
     2581 gctaacaact gcaccttcga gtacgtgtct cagcctttcc tgatggacct ggaaggaaag
     2641 cagggaaact tcaagaacct gagagagttc gtgttcaaga acatcgacgg ttacttcaag
     2701 atatacagca agcacacccc aatcaacctg gtgagggacc tgccacaggg tttctctgct
     2761 ctggagcccc tggtggacct gccaatcggc atcaacatca ccaggttcca gaccctgctg
     2821 gctctgcaca ggtcttacct gaccccaggc gacagcagct ctggatggac cgccggcgcc
     2881 gctgcttact acgtgggata cctgcagcca aggaccttcc tgctgaagta caacgaaaac
     2941 ggcaccatca ccgacgccgt ggactgcgct ctggacccac tgtctgagac caagtgcacc
     3001 ctgaagtctt tcaccgtgga aaagggaatc taccagacca gcaacttccg cgtgcagccc
     3061 accgagtcta tcgtgagatt cccaaacatc accaacctgt gcccattcgg agaagtgttc
     3121 aacgctacca ggttcgccag cgtgtacgct tggaacagga agagaatcag caactgcgtg
     3181 gccgactact ctgtgctgta caactctgcc tccttctcca ccttcaagtg ctacggcgtg
     3241 tccccaacca agctgaacga cctgtgcttc accaacgtgt acgctgactc cttcgtgatc
     3301 agaggagacg aagtgagaca gatcgctccc ggtcagaccg gaaagatcgc cgactacaac
     3361 tacaagctgc cagacgactt caccggttgc gtgatcgctt ggaactctaa caacctggac
     3421 tctaaggtgg gaggcaacta caactacctg tacaggctgt tccgcaagag caacctgaag
     3481 cctttcgaaa gggacatcag caccgaaatc taccaggctg gatctacccc atgcaacgga
     3541 gtggaaggat tcaactgcta cttccctctg cagtcttacg gattccagcc aaccaacgga
     3601 gtgggatacc agccatacag agtggtggtg ctgtctttcg agctgctgca cgctcctgct
     3661 accgtgtgcg gaccaaagaa gtctaccaac ctggtgaaga acaagtgcgt gaacttcaac
     3721 ttcaacggac tgaccggaac cggagtgctg accgaatcca acaagaagtt cctgcctttc
     3781 cagcagttcg gtagagacat cgccgacacc accgacgctg tgagagaccc acagaccctg
     3841 gaaatcctgg acatcacccc ctgctctttc ggaggagtga gcgtgatcac cccaggaacc
     3901 aacacctcca accaggtggc cgtgctgtac caggacgtga actgcaccga agtgccagtg
     3961 gccatccacg ctgaccagct gaccccaacc tggagagtgt acagcaccgg atctaacgtg
     4021 ttccagaccc gtgccggctg cctgatcgga gccgagcacg tgaacaactc ctacgaatgc
     4081 gacatcccaa tcggagctgg catctgcgct tcttaccaga cccagaccaa cagcccaaga
     4141 agggccagat ccgtggcctc tcagagcatc atcgcctaca ccatgagcct gggcgctgaa
     4201 aacagcgtgg cttacagcaa caactctatc gccatcccaa ccaacttcac catctctgtg
     4261 accaccgaaa tcctgccagt gagcatgacc aagaccagcg tggactgcac catgtacatc
     4321 tgcggagact ctaccgaatg ctctaacctg ctgctgcagt acggcagctt ctgcacccag
     4381 ctgaaccgcg ctctgaccgg aatcgctgtg gagcaggaca agaacaccca ggaagtgttc
     4441 gctcaggtga agcagatata caagacccca cctatcaagg acttcggagg attcaacttc
     4501 tctcagatcc tgcccgaccc atctaagccc tctaagaggt ctttcatcga agacctgctg
     4561 ttcaacaagg tgaccctggc cgacgccggt ttcatcaagc agtacggtga ctgcctggga
     4621 gacatcgccg ctagagacct gatctgcgcc cagaagttca acggtctgac cgtgctgcca
     4681 ccactgctga ccgacgagat gatcgcccag tacacctctg ccctgctggc tggaaccatc
     4741 acctctggtt ggaccttcgg agctggcgcc gccctgcaga tcccattcgc tatgcagatg
     4801 gcctaccgtt tcaacggtat cggagtgacc cagaacgtgc tgtacgaaaa ccagaagctg
     4861 atcgctaacc agttcaactc tgctatcgga aagatccagg actccctgtc ctctaccgct
     4921 tctgccctgg gaaagctgca ggacgtggtg aaccagaacg cccaggccct gaacaccctg
     4981 gtgaagcagc tgtcttctaa cttcggagcc atctcctctg tgctgaacga catcctgtct
     5041 aggctggaca aggtggaagc cgaggtgcag atcgacagac tgatcaccgg aagactgcag
     5101 tctctgcaga cctacgtgac ccagcagctg atcagggccg ctgaaatcag agcttctgcc
     5161 aacctggctg ccatcaagat gtccgaatgc gtgctgggcc agtctaagag ggtggacttc
     5221 tgcggaaagg gataccacct gatgtccttc ccacagtctg ctccacacgg agtggtgttc
     5281 ctgcacgtga cctacgtgcc cgcccaggaa aagaacttca ccaccgcccc agctatctgc
     5341 cacgacggaa aggctcactt ccccagggag ggagtgttcg tgtctaacgg aacccactgg
     5401 ttcgtgaccc agaggaactt ctacgagcca cagatcatca ccaccgacaa caccttcgtg
     5461 tctggaaact gcgacgtggt gatcggaatc gtgaacaaca ccgtgtacga cccactgcag
     5521 ccagaactgg actctttcaa ggaggaactg gacaagtact tcaagaacca cacctctcca
     5581 gacgtggacc tgggagacat ctccggaatc aacgcctctg tggtgaacat ccagaaggaa
     5641 atcgacagac tgaacgaggt ggccaagaac ctgaacgaat ctctgatcga cctgcaggaa
     5701 ctgggaaagt acgagcagta catcaagtgg ccctggtaca tctggctggg tttcatcgct
     5761 ggtctgatcg ctatcgtgat ggtgaccatc atgctgtgct gcatgacctc ttgctgctct
     5821 tgcctgaagg gatgctgctc ttgcggatct tgctgcaagt tcgacgagga cgactccgag
     5881 cctgtgctga agggtgtgaa gctgcactac acctacccaa ctttcttgta caaagtggtt
     5941 gatatcaagg ccgccgacat caccagcctg tacaagaaag tggggtggag ccatccccag
     6001 ttcgagaagg gcggaggatc tggcggcgga agcggcggag gaagctggtc ccaccctcag
     6061 tttgagaaag gcaccgagct gggcagcacc atggccagct acccctacga cgtgcccgac
     6121 tacgccaagg acaacaccgt gcccctgaag ctgatcgccc tgctggccaa cggcgagttc
     6181 cactctggcg agcagctggg cgagacactg ggcatgagca gagccgccat caacaagcac
     6241 atccagaccc tgcgggactg gggcgtggac gtgttcacag tgcccggcaa gggctacagc
     6301 ctgcccgagc ccatccagct gctgaacgcc aagcagatcc tgggccagct ggatggcgga
     6361 tctgtggccg tgctgcccgt gatcgacagc accaaccagt atctgctcga ccggatcggc
     6421 gagctgaagt ccggcgacgc ctgtatcgcc gagtaccagc aggccggcag aggcggcagg
     6481 ggcagaaagt ggttcagccc cttcggcgcc aacctgtacc tgagcatgtt ctggagactg
     6541 gaacagggcc cagccgccgc tattggcctg agcctggtca tcggcatcgt gatggccgag
     6601 gtgctgcgga agctgggcgc cgacaaagtg cgcgtgaagt ggcccaacga cctgtatctg
     6661 caggaccgga agctggccgg catcctggtg gaactgaccg gcaaaacagg cgacgccgcc
     6721 cagatcgtga tcggcgctgg catcaacatg gccatgcggc gggtggaaga gagcgtggtc
     6781 aaccagggct ggatcaccct gcaggaagcc ggcatcaacc tggaccggaa caccctggcc
     6841 gccatgctga tcagagagct gagagccgcc ctggaactgt tcgagcagga aggcctggcc
     6901 ccctacctga gcagatggga gaagctggac aacttcatca accggcccgt gaaactgatc
     6961 atcggcgaca aagagatctt cggcatcagc cggggcatcg acaagcaggg ggccctgctc
     7021 ctggaacagg acggcatcat caagccctgg atgggcggcg agatcagcct gcggagcgcc
     7081 gagaagtgat gactcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     7141 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7201 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7261 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7321 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     7381 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7441 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7501 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7561 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7621 tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt
     7681 ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     7741 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     7801 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     7861 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     7921 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     7981 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     8041 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     8101 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     8161 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     8221 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     8281 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     8341 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     8401 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     8461 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     8521 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     8581 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     8641 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     8701 cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat
     8761 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8821 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     8881 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8941 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     9001 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9061 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9121 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9181 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9241 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9301 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9361 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9421 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9481 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9541 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9601 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9661 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9721 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9781 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9841 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9901 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9961 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10021 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    10081 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10141 aaa
//
