LOCUS       sequence-384239-        9128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for generating multiple sgRNAs carrying plasmids 
            by Golden Gate Assembly..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Cao J, Wu L, Zhang SM, Lu M, Cheung WK, Cai W, Gale M, Xu Q, Yan Q
  TITLE     An easy and efficient inducible CRISPR/Cas9 platform with improved 
            specificity for multiple gene targeting.
  JOURNAL   Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25.
  PUBMED    27458201
REFERENCE   2  (bases 1 to 9128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-11-2"; volume: "44(19)"; pages: "e149. Epub
            2016 Jul 25"
COMMENT     Sequence Label: Lenti-entry-puro
FEATURES             Location/Qualifiers
     source          1..9128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1192..1791
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1192..1211)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1688..1708
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1807..2395
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1860..1880)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2278..2289)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2467..2700
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2778..2899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2815..2834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2869..2888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2939..3074
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3094..3113)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3095..3113)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3123..3140)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3123..3139)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3132..3154)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3281..3736
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3368..3387)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3578..3599
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3762..3866
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3867..3935,3936..4727)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4085..4104)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4898..5486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5387..5406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5640..5657
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5774..5795
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5810..5815,5816..5833,5834..5840)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5848..5864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5853..5875
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5872..5888
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5872..5888
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5907..5927
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5909..5927
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5955..6181
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6182..6362
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6409..6534
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7027..7260
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        join(7412..7417,7418..7435,7436..7442)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7496..8155
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7562..7581)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8497..8802
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8681..8700
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    8873..8990
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgac gtacggccac catgaccgag
     1201 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     1261 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     1321 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     1381 gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa
     1441 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     1501 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     1561 ttcctggcca ccgtcggagt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     1621 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     1681 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     1741 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aacgcgttaa
     1801 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     1861 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     1921 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     1981 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2041 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2101 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2161 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     2221 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2281 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2341 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     2401 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     2461 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     2521 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     2581 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     2641 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     2701 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     2761 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2821 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2881 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     2941 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3001 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3061 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     3121 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3181 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3241 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     3301 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3361 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3421 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3481 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3541 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3601 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3661 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3721 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3781 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     3841 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     3901 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     3961 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4021 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4081 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4141 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4201 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4261 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4321 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4381 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4441 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4501 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4561 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4621 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     4681 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     4741 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4801 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4861 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4921 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4981 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5041 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5101 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5161 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5221 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5281 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5341 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5401 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5461 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5521 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5581 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     5641 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     5701 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     5761 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     5821 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     5881 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     5941 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     6001 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     6061 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     6121 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     6181 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6241 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6301 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6361 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     6421 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     6481 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     6541 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     6601 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     6661 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     6721 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     6781 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     6841 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     6901 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     6961 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     7021 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     7081 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     7141 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     7201 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     7261 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     7321 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     7381 agaagataag agacgattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
     7441 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga
     7501 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
     7561 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
     7621 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     7681 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
     7741 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     7801 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     7861 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     7921 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     7981 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
     8041 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
     8101 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaagatc
     8161 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     8221 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     8281 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     8341 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     8401 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     8461 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     8521 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     8581 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     8641 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     8701 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     8761 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     8821 cacagccagt ctgcagcgtc tccacaagac aaatggcagt attcatccac aattttaaaa
     8881 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     8941 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9001 acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccgggtg caaagatgga
     9061 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
     9121 tgggaatt
//
