LOCUS       sequence-384241-         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the triple Myc-tagged mouse AP-4 sigma-4 subunit in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Mattera R, De Pace R, Bonifacino JS
  TITLE     The adaptor protein chaperone AAGAB stabilizes AP-4 complex 
            subunits.
  JOURNAL   Mol Biol Cell. 2022 Oct 1;33(12):ar109. doi: 
            10.1091/mbc.E22-05-0177. Epub 2022 Aug 17.
  PUBMED    35976721
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2022-10-1"; volume: "33(12):ar109. doi"; pages: " 
            10.1091/mbc.E22-05-0177. Epub 2022 Aug 17"
COMMENT     Sequence Label: pCI-neo-AP-4 (Myc)3-sigma-4
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..69
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             73..102
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             109..138
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 taatacgact cactataggc tagcctcgag gccaccatgg agcagaagct gatctccgaa
       61 gaggacctcg gcgaacagaa actcatctct gaggaagatc tgatggggga acagaagctg
      121 atttccgagg aagacctgga attcggatct ggttcgggag gctctggatc aggtatgatc
      181 aagtttttcc ttatggtgaa caagcaaggg cagacccgac tgtctaagta ctacgagcat
      241 gtggacatta ataaacgtgc gcttctggag actgaggtca gcaagagttg cctgtctcgg
      301 tccagcgaac aatgctcatt cattgaatac aaggatttta aactgatcta ccggcaatat
      361 gcagctctct ttgttgtggt tggagttaat gacactgaga atgagatggc tatctatgaa
      421 tttatacaca attttgtgga agttttagat gggtacttca gccgagtgag tgaattagat
      481 ataatgttta atttggataa agttcacatc attttggatg agatggtgtt aaatggctgc
      541 attgtggaaa ctaacagagc cagaatactt gcccctctgc tgattcttga caagctgtcg
      601 gaaagctgag cggccgcttc cctttagtga gggttaatg
//
