LOCUS       sequence-384253-       11080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Integratable, GZV-inducible expression of synZiFTR: 
            ZF10-p65.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   Li HS, Israni DV, Gagnon KA, Gan KA, Raymond MH, Sander JD, Roybal 
            KT, Joung JK, Wong WW, Khalil AS
  TITLE     Multidimensional control of therapeutic human cell function with 
            synthetic gene circuits.
  JOURNAL   Science. 2022 Dec 16;378(6625):1227-1234. doi: 
            10.1126/science.ade0156. Epub 2022 Dec 15.
  PUBMED    36520914
REFERENCE   2  (bases 1 to 11080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.ade0156"; journalName: "Science"; date: 
            "2022-12-16- 16"; volume: "378"; issue: "6625"; pages: "1227-1234"
COMMENT     Sequence Label: pHS-139
FEATURES             Location/Qualifiers
     source          1..11080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1002..1067
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1197..1217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1642..1776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1679..1698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1733..1752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1944..2577
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2624..2749
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3246..3479
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3664..3708
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3993..4110
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4263..4670
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5253..5318
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5325..5345
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6195..6221
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6447..6818
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    6887..7475
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6940..6960)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7358..7369)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7478..7494)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7479..7495)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7585..7818
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7843..7861)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7844..7861)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8151..8170)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8384..8403)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8503..8525
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8563..8581)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8649..8753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8754..8822,8823..9614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8972..8991)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9785..10373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10274..10293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10527..10544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10614..10634)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10619..10948
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10799..10934
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
       61 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
      121 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
      181 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
      241 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
      301 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
      361 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
      421 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
      481 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
      541 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
      601 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
      661 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
      721 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
      781 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
      841 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
      901 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
      961 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     1021 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     1081 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     1141 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     1201 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     1261 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     1321 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     1381 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     1441 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     1501 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     1561 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     1621 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     1681 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1741 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     1801 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     1861 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     1921 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     1981 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     2041 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     2101 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     2161 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     2221 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     2281 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     2341 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     2401 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2461 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2521 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     2581 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     2641 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     2701 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     2761 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     2821 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     2881 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     2941 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     3001 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     3061 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     3121 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3181 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     3241 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     3301 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     3361 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     3421 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     3481 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     3541 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     3601 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     3661 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     3721 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     3781 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     3841 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     3901 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     3961 cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     4021 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4081 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     4141 gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa aagattttat
     4201 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctg
     4261 cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag aagttcagat
     4321 caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg cggtgagcag
     4381 tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc ccggcccgag
     4441 gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta agacccatca
     4501 gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg aattaaccaa
     4561 tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc
     4621 acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg ggggatctgg
     4681 agctctcgag aattctcacg cgtgccgcca ccatgtctag acccggagag cgcccattcc
     4741 agtgtcggat ttgcatgcgg aacttttcga gaagacacgg cctggacaga catacccgta
     4801 ctcatacagg tgaaaaaccc tttcagtgtc ggatctgtat gcgaaatttc tccgaccaca
     4861 gcagcctgaa gagacatcta cgtacccaca ccggcagcca gaagccattt cagtgtcgga
     4921 tctgtatgcg gaacttctcc gtgagacaca acctgaccag acatctacgt acgcacaccg
     4981 gagagaagcc attccaatgc cgaatatgca tgcgcaactt cagtgaccac agcaacctga
     5041 gcagacacct aaaaacccac accggttccc agaagccatt tcagtgtcgg atctgtatgc
     5101 ggaacttctc ccagcgcagc agcctggtga gacatctacg tacgcacacc ggagagaagc
     5161 cattccaatg ccgaatatgc atgcgcaact tcagtgagag cggccacctg aagagacacc
     5221 tgcgtacgca cctgagggga tccacctgca gggactacaa agaccatgac ggtgattata
     5281 aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag aaaaagagga
     5341 aggtgggcat tcacggggtg ccgggtggac tcgagggagg cggtggaagc ggcggtaccg
     5401 aggacgtggt gtgctgccac tcaatctacg gcaagaagaa gggtgatatc gacacctacc
     5461 gatacatagg ctcttccggg acaggctgcg tggtcatagt gggcaggatc gtcttgtccg
     5521 gatccggcac tagtgcgccc atcacggcgt acgcccagca gacgagaggc ctcctagggt
     5581 gtataatcac cagcctgact ggccgggaca aaaaccaagt ggagggtgag gtccagatcg
     5641 tgtcaactgc tacccaaacc ttcctggcaa cgtgcatcaa tggggtatgc tgggcagtct
     5701 accacggggc cggaacgagg accatcgcat cacccaaggg tcctgtcatc cagatgtata
     5761 ccaatgtgga ccaagacctt gtgggctggc ccgctcctca aggttcccgc tcattgacac
     5821 cctgtacctg cggctcctcg gacctttacc tggtcacgag gcacgccgat gtcattcccg
     5881 tgcgccggcg aggtgatagc aggggtagcc tgctttcgcc ccggcccatt tcctacttga
     5941 aaggctcctc ggggggtccg ctgttgtgcc ccgcgggaca cgccgtgggc ctattcaggg
     6001 ccgcggtgtg cacccgtgga gtggctaaag cggtggactt tatccctgtg gagaacctag
     6061 agacaaccat gagatccccg gtgttcacgg acaactcctc tccaccagca gtcaccctga
     6121 cgcacccaat caccaaaatc gatagggagg ttctctacca ggagttcgat gagatggaag
     6181 agtgctctca gcactatccc tacgatgtgc ccgattacgc tggaggcggt ggaagcggcg
     6241 gtaccgatga gtttcccacc atggtgtttc cttctgggca gatcagccag gcctcggcct
     6301 tggccccggc ccctccccaa gtcctgcccc aggctccagc ccctgcccct gctccagcca
     6361 tggtatcagc tctggcccag gccccagccc ctgtcccagt cctagcccca ggccctcctc
     6421 aggctgtggc cccacctgcc cccaagccca cccaggctgg ggaaggaacg ctgtcagagg
     6481 ccctgctgca gctgcagttt gatgatgaag acctgggggc cttgcttggc aacagcacag
     6541 acccagctgt gttcacagac ctggcatccg tcgacaactc cgagtttcag cagctgctga
     6601 accagggcat acctgtggcc ccccacacaa ctgagcccat gctgatggag taccctgagg
     6661 ctataactcg cctagtgaca ggggcccaga ggccccccga cccagctcct gctccactgg
     6721 gggccccggg gctccccaat ggcctccttt caggagatga agacttctcc tccattgcgg
     6781 acatggactt ctcagccctg ctgagtcaga tcagctccca attgtaagcg gccgcgactc
     6841 tagagtcgac ctgcaggcat gcaagcttga tatcaagctt atcgataatc aacctctgga
     6901 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6961 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7021 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7081 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7141 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7201 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7261 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     7321 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7381 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7441 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagggaa
     7501 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7561 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7621 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7681 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7741 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7801 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
     7861 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
     7921 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
     7981 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
     8041 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
     8101 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     8161 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     8221 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     8281 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     8341 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     8401 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     8461 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     8521 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     8581 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     8641 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8701 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8761 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8821 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8881 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8941 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9001 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9061 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9121 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9181 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9241 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9301 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9361 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9421 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9481 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9541 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9601 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9661 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9721 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9781 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9841 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9901 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9961 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10021 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10081 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10141 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10201 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10261 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10321 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10381 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10441 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10501 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    10561 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
    10621 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    10681 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
    10741 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
    10801 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    10861 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    10921 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
    10981 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
    11041 catgtgaaat actggttttt agtgcgccag atctctataa
//
