LOCUS       sequence-384256-       12831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Integratable Inducible synZiFTR ZF1 + Reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12831)
  AUTHORS   Li HS, Israni DV, Gagnon KA, Gan KA, Raymond MH, Sander JD, Roybal 
            KT, Joung JK, Wong WW, Khalil AS
  TITLE     Multidimensional control of therapeutic human cell function with 
            synthetic gene circuits.
  JOURNAL   Science. 2022 Dec 16;378(6625):1227-1234. doi: 
            10.1126/science.ade0156. Epub 2022 Dec 15.
  PUBMED    36520914
REFERENCE   2  (bases 1 to 12831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.ade0156"; journalName: "Science"; date: 
            "2022-12-16- 16"; volume: "378"; issue: "6625"; pages: "1227-1234"
COMMENT     Sequence Label: pMN-332
FEATURES             Location/Qualifiers
     source          1..12831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1002..1067
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1197..1217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1642..1776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1679..1698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1733..1752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1944..2577
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2624..2749
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3246..3479
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3664..3708
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3993..4110
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4179..4889)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWQASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4457..4476)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4726..4744
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      4886..4895
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5299..5316)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     promoter        5436..5843
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5876..5896
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7346..7366
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7385..7441
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7652..8023
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    8638..9226
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8691..8711)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9109..9120)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9229..9245)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9230..9246)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9336..9569
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9594..9612)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9595..9612)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9902..9921)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10135..10154)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10254..10276
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10314..10332)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10400..10504
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10505..10573,10574..11365)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10723..10742)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11536..12124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12025..12044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12278..12295
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12365..12385)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12370..12699
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12550..12685
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
       61 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
      121 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
      181 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
      241 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
      301 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
      361 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
      421 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
      481 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
      541 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
      601 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
      661 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
      721 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
      781 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
      841 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
      901 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
      961 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     1021 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     1081 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     1141 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     1201 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     1261 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     1321 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     1381 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     1441 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     1501 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     1561 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     1621 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     1681 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1741 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     1801 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     1861 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     1921 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     1981 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     2041 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     2101 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     2161 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     2221 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     2281 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     2341 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     2401 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2461 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2521 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     2581 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     2641 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     2701 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     2761 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     2821 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     2881 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     2941 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     3001 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     3061 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     3121 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3181 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     3241 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     3301 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     3361 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     3421 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     3481 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     3541 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     3601 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     3661 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     3721 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     3781 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     3841 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     3901 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     3961 cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     4021 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4081 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     4141 gtttggatcg ataagcttga tatcgaattc ctgcagcctt acttgtacag ctcgtccatg
     4201 ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg
     4261 ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc
     4321 ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc
     4381 agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag
     4441 gcctgccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa gttggtgccg
     4501 cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc
     4561 accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac
     4621 agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtacatg
     4681 aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc
     4741 ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg
     4801 tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt gatgatggcc
     4861 atgttatcct cctcgccctt gctcaccatg gtggcgatcc ggtagcgcta gcgatgagct
     4921 ctggtagtag actagtggcc cccattatat accctctaga ggatcctatc cactccgtgt
     4981 aagggagagt gagcctctta cgaatggtcg acttcgactt cgacgttccg aggcgactga
     5041 tacggtcgac ttcgacttcg acgtacgaag gcagtccgat tggtcgactt cgacttcgac
     5101 gacctttact gagacgggag gtcgacttcg acttcgacga aggcgttgcg aatcctcatg
     5161 cgattgttac gaaacccggg tcgacttcga cttcgacgta ttggaagcac ggctctggtc
     5221 gacttcgact tcgacgacct cgggagaact gattggtcga cttcgacttc gacgaagagg
     5281 ttgacgctat ccggtcgact tcgacttcga cgagccctgt aggtagacta gatacagtgt
     5341 tactcaatcg tccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     5401 aagaccccac ctgtaggttt ggcaagctag ctgcagtaac gccattttgc aaggcatgga
     5461 aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga aaatagctaa
     5521 cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg ggccaagaac
     5581 agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc cccagatatg
     5641 gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc ccaaggacct
     5701 gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct tctgttcgcg
     5761 cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc gcgccagtcc
     5821 tccgacagac tgagtcgccc gggacgcgtt taattaagcc gccaccatgt tcaaacccaa
     5881 gaagaagaga aaggtgttcg aaactagtgt gcccctgtat ggcttcactt ccatttgtgg
     5941 ccgacggcct gaaatggaag ccgcggtgtc aaccatacca cggtttctgc agagctcatc
     6001 aggctccatg ctggacggac gctttgatcc acagtctgcc gcacatttct ttggagtcta
     6061 cgacggccac gggggcagcc aggtcgccaa ctactgcagg gaaaggatgc atttggcact
     6121 tgccgaagag atcgccaaag agaagcccat gttgtgtgat ggggatacct ggctggagaa
     6181 gtggaagaaa gcgcttttta actcttttct gagagtggat tctgagatag aatctgtcgc
     6241 acccgagacc gtgggcagca catccgtcgt agccgtagtg tttccctccc acatattcgt
     6301 cgccaactgc ggcgacagtc gagccgtcct ctgccgaggt aagaccgccc tgcctctgag
     6361 tgttgaccat aagcccgacc gggaggatga ggccgcccga atcgaggccg ccggtggaaa
     6421 agtcatccaa tggaacggcg caagagtgtt cggcgtgctg gcgatgtcca ggagcattgg
     6481 agaccggtac ctgaagccca gcataatccc agatcccgaa gtgaccgcag tcaagagggt
     6541 gaaagaggac gattgtctga tcctggctag cgatggcgta tgggacgtga tgactgatga
     6601 ggaggcgtgt gaaatggccc gcaagcgaat cctgctgtgg cataaaaaaa acgcagtcgc
     6661 gggggacgct tctcttctgg cagacgaaag gcgcaaagaa ggtaaagacc cggctgctat
     6721 gagcgccgcc gaatatctca gtaagctggc aattcagcga gggtccaaag acaacatttc
     6781 cgtggtcgtg gtagacctca aaggcggttc cggcggttct agaccaggcg aacgaccgtt
     6841 tcaatgccgg atatgtatga ggaacttctc cgaggaggca aacttgaggc gccacacccg
     6901 aacacataca ggagaaaagc cattccaatg tcgaatttgt atgcgcaatt tttcagatca
     6961 ctcaagcctc aagcgacacc tccgcacaca tactggttca cagaagccct ttcagtgcag
     7021 gatttgtatg cgcaacttta gccaatcagc gaaccttttg cggcacacta gaacgcatac
     7081 aggtgagaag cctttccagt gtcgcatctg tatgcggaac ttcagcgacc ccagttcatt
     7141 gaagaggcat ttgcgaactc acaccggttc tcaaaaacct tttcagtgcc gaatttgtat
     7201 gcgcaacttc agccaacaaa caaatttgac gagacacacg cgcacgcaca ccggggaaaa
     7261 accgtttcaa tgtcgaatct gcatgcgcaa ttttagcgat gcgacacaac ttgttaggca
     7321 tctgcgcaca cacttgcggg gatccccgaa gaaaaaacgg aaagtgacct gcaggggaag
     7381 cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc
     7441 tggtcaccat gatgagtttc ccaccatggt gtttccttct gggcagatca gccaggcctc
     7501 ggccttggcc ccggcccctc cccaagtcct gccccaggct ccagcccctg cccctgctcc
     7561 agccatggta tcagctctgg cccaggcccc agcccctgtc ccagtcctag ccccaggccc
     7621 tcctcaggct gtggccccac ctgcccccaa gcccacccag gctggggaag gaacgctgtc
     7681 agaggccctg ctgcagctgc agtttgatga tgaagacctg ggggccttgc ttggcaacag
     7741 cacagaccca gctgtgttca cagacctggc atccgtcgac aactccgagt ttcagcagct
     7801 gctgaaccag ggcatacctg tggcccccca cacaactgag cccatgctga tggagtaccc
     7861 tgaggctata actcgcctag tgacaggggc ccagaggccc cccgacccag ctcctgctcc
     7921 actgggggcc ccggggctcc ccaatggcct cctttcagga gatgaagact tctcctccat
     7981 tgcggacatg gacttctcag ccctgctgag tcagatcagc tccggtggtg gcagcggtca
     8041 attgactcaa gacgaattca cccaactctc ccaatcaatc gccgagttcc acacgtacca
     8101 actcggtaac ggccgttgct catctctcct agctcagcga atccacgcgc cgccggaaac
     8161 agtatggtcc gtggtgagac gtttcgatag gccacagatt tacaaacact tcatcaaaag
     8221 ctgtaacgtg agtgaagatt tcgagatgcg agtgggatgc acgcgcgacg tgaacgtgat
     8281 aagtggatta ccggcgaata cgtctcgaga gagattagat ctgttggacg atgatcggag
     8341 agtgactggg tttagtataa ccggtggtga acataggctg aggaattata aatcggttac
     8401 gacggttcat agatttgaga aagaagaaga agaagaaagg atctggaccg ttgttttgga
     8461 atcttatgtt gttgatgtac cggaaggtaa ttcggaggaa gatacgagat tgtttgctga
     8521 tacggttatt agattgaatc ttcagaaact tgcttcgatc actgaagcta tgaactaagc
     8581 ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct tatcgataat
     8641 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8701 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8761 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8821 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8881 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8941 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     9001 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     9061 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     9121 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     9181 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     9241 cctcgaggga attaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     9301 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     9361 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     9421 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9481 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9541 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     9601 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     9661 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     9721 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     9781 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     9841 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     9901 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     9961 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
    10021 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
    10081 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
    10141 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
    10201 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
    10261 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
    10321 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
    10381 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    10441 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    10501 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    10561 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    10621 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    10681 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    10741 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    10801 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    10861 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    10921 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    10981 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    11041 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    11101 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    11161 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    11221 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    11281 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    11341 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    11401 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    11461 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    11521 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    11581 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    11641 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    11701 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    11761 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    11821 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    11881 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    11941 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    12001 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    12061 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    12121 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    12181 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    12241 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    12301 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    12361 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
    12421 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    12481 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    12541 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    12601 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    12661 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
    12721 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
    12781 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata a
//
