LOCUS       sequence-384266-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-1 
            sigma-1A fusion protein in yeast (yeast two-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Mattera R, De Pace R, Bonifacino JS
  TITLE     The adaptor protein chaperone AAGAB stabilizes AP-4 complex 
            subunits.
  JOURNAL   Mol Biol Cell. 2022 Oct 1;33(12):ar109. doi: 
            10.1091/mbc.E22-05-0177. Epub 2022 Aug 17.
  PUBMED    35976721
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2022-10-1"; volume: "33(12):ar109. doi"; pages: " 
            10.1091/mbc.E22-05-0177. Epub 2022 Aug 17"
COMMENT     Sequence Label: pGADT7-AP-1 sigma-1A
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             85..111
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctattcgatg atgaagatac cccaccaaac ccaaaaaaag agatctttaa tacgactcac
       61 tatagggcga gcgccgccat ggagtaccca tacgacgtac cagattacgc tcatatggcc
      121 atggaggcca gtgaattcat gatgcggttc atgctattat tcagccggca gggaaaactg
      181 cggctgcaaa aatggtacct ggccacttcg gacaaggaac ggaagaagat ggtgcgcgag
      241 ctcatgcagg ttgtcctggc tcgaaagccc aagatgtgca gcttcctgga gtggagggac
      301 ctcaaagttg tctataagag atatgccagc ctctacttct gctgcgccat cgagggccaa
      361 gacaatgagc tcatcacact ggagctgatc caccgatacg tggagctctt agacaaatac
      421 tttggcagtg tgtgcgagct ggacatcatc ttcaactttg agaaggccta cttcatcctg
      481 gatgagtttt tgatgggggg ggatgtccag gacacctcca agaagagtgt gctgaaagcc
      541 atcgagcagg ctgacctact gcaagaggag gatgagtcgc cacggagtgt gctggaggag
      601 atgggtttgg cactcgagct gcagatgaat cgtagatact gaaaaacccc gcaagttcac
      661 ttcaactgtg catcgtgcac catct
//
