LOCUS       sequence-384272-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-1 
            sigma-1B fusion protein in yeast (yeast two-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Mattera R, De Pace R, Bonifacino JS
  TITLE     The adaptor protein chaperone AAGAB stabilizes AP-4 complex 
            subunits.
  JOURNAL   Mol Biol Cell. 2022 Oct 1;33(12):ar109. doi: 
            10.1091/mbc.E22-05-0177. Epub 2022 Aug 17.
  PUBMED    35976721
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2022-10-1"; volume: "33(12):ar109. doi"; pages: " 
            10.1091/mbc.E22-05-0177. Epub 2022 Aug 17"
COMMENT     Sequence Label: pGADT7-AP-1 sigma-1B
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             85..111
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctattcgatg atgaagatac cccaccaaac ccaaaaaaag agatctttaa tacgactcac
       61 tatagggcga gcgccgccat ggagtaccca tacgacgtac cagattacgc tcatatggcc
      121 atggaggcca gtgaattcat gcagtttatg ttgcttttta gtcgtcaggg aaagcttcga
      181 ctgcaaaaat ggtatgtccc actatcagac aaagagaaga aaaagatcac aagagaactt
      241 gttcagaccg ttttagcacg gaaacctaaa atgtgcagct tccttgagtg gcgagatctg
      301 aagattgttt acaaaagata tgctagtctg tatttttgct gtgctattga ggatcaggac
      361 aatgaactaa ttaccctgga aataattcat cgttatgtgg aattacttga caagtatttc
      421 ggcagtgtct gtgaactaga tatcatcttt aattttgaga aggcttattt tattttggat
      481 gagtttcttt tgggagggga agttcaggaa acatccaaga aaaatgtcct taaagcaatt
      541 gagcaggctg atctactgca ggaggaagct gaaaccccac gtagtgttct tgaagaaatt
      601 ggactgacac tcgagctgca gatgaatcgt agatactgaa aaaccccgca agttcacttc
      661 aactgtgcat cgtgcaccat c
//
