LOCUS       sequence-38429-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Nemoto S, Finkel T
  TITLE     Redox regulation of forkhead proteins through a p66shc-dependent 
            signaling pathway.
  JOURNAL   Science. 2002 Mar 29. 295(5564):2450-2.
  PUBMED    11884717
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2002 Mar 29. 295(5564):2450-2."
COMMENT     Sequence Label: pcDNA3.1his p66shc S36A (corrected)
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             115..132
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             136..168
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(172..180,181..195)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagctta ccatgggggg ttctcatcat
      121 catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac
      181 gacgatgacg ataaggtacc gagctcggat ccactagtcc agtgtggtgg aattcggctt
      241 atggatctcc tgccccccaa gcccaagtac aatccactcc ggaatgagtc tctgtcatcg
      301 ctggaggaag gggcttctgg gtccaccccc ccggaggagc tgcctgcccc atcagcttca
      361 tccctggggc ccatcctgcc tcctctgcct ggggacgata gtcccactac cctgtgctcc
      421 ttcttccccc ggatgagcaa cctgaggctg gccaacccgg ctggggggcg cccagggtct
      481 aagggggagc caggaagggc agctgatgat ggggagggga tcgtaggggc agccatgcca
      541 gactcaggcc ccctacccct cctccaggac atgaacaagc tgagtggagg cggcgggcgc
      601 aggactcggg tggaaggggg ccagcttggg ggcgaggagt ggacccgcca cgggagcttt
      661 gtcaataagc ccacgcgggg ctggctgcat cccaacgaca aagtcatggg acccggggtt
      721 tcctacttgg ttcggtacat gggttgtgtg gaggtcctcc agtcaatgcg tgccctggac
      781 ttcaacaccc ggactcaggt caccagg
//
