LOCUS       sequence-384302-        7666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSLI-TGD/TurboID, C-terminal tag of recodonized TurboID for 
            Plasmodium falciparum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Bennink S, Lindner SE
  TITLE     Bennink et al - PfTurboID
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSL1423
FEATURES             Location/Qualifiers
     source          1..7666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1015..1043)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1638..1658
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1708..1761
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1756..2556
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1816..1835)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2426..2445
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4977..4996)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4978..4996)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5003..5020)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5003..5019)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5012..5034)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5228..5247)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5347..5369
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5407..5425)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5493..5597
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5598..5666,5667..6458)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5816..5835)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6629..7217
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7118..7137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7371..7388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7505..7526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7541..7546,7547..7564,7565..7571)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7579..7595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7584..7606
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7603..7619
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7603..7619
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7637..7655
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7637..7654
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gcggccgctc tcgagactag tatgaaagat aacacggttc ccttaaaatt gatagccctt
       61 cttgccaatg gtgagtttca ttccggggaa caacttggcg aaactctggg aatgagcagg
      121 gcagcgatta acaagcatat ccaaaccttg agagactggg gagtagatgt gttcaccgtg
      181 cctggtaaag gttattcttt gccggaacct atccctctat taaatgcgaa acagatacta
      241 ggacaactgg acggtggtag tgttgccgta ttgccggttg ttgactcaac caatcaatac
      301 ttgctggaca gaattggcga acttaaaagc ggtgatgcgt gtattgcaga atatcagcaa
      361 gcagggagag gctcaagagg taggaaatgg ttttcacctt ttggcgccaa tctttactta
      421 agtatgttct ggcgtttgaa gagaggacct gcggctattg gtttaggtcc cgttatcggg
      481 atagttatgg ctgaagcgct acgtaaactt ggggccgaca aggtcagagt gaagtggcct
      541 aatgatctat acttgcaaga tagaaagctg gcgggtatcc tggtggaact agcgggaatc
      601 acaggggacg ctgcccaaat agtaatcggt gctggtataa atgtcgcaat gcgtagagtc
      661 gaggaatcag tggtgaacca gggctggatc acgttgcaag aggcgggcat aaacctggac
      721 cgtaacaccc ttgcagcgac cctaataaga gaactacgtg ctgcattgga gctttttgag
      781 caggagggcc tggctccgta cctacccagg tgggagaaat tagacaattt cataaaccgt
      841 ccagtcaaac taataattgg agataaggag attttcggta tttctcgtgg gatagacaag
      901 cagggagcgt tgttattaga acaggatggt gtcattaaac cttggatggg cggtgaaatt
      961 agtcttcgta gtgccgaaaa gtctagtagt aaaggagaag aacttttcac tggagttgtc
     1021 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
     1081 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
     1141 ctacctgttc catggccaac acttgtcact actttcgcgt atggtcttca atgctttgcg
     1201 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
     1261 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
     1321 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
     1381 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
     1441 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
     1501 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
     1561 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
     1621 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
     1681 atggatgagc tctacaaagt cgacggagaa ggaagaggaa gtttattaac atgtggagat
     1741 gtagaagaaa atccaggacc aatgattgaa caagatggat tgcacgcagg ttctccggcc
     1801 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     1861 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     1921 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     1981 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     2041 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     2101 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     2161 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     2221 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     2281 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     2341 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     2401 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     2461 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     2521 atcgccttct atcgccttct tgacgagttc ttctaactcg agggatatgg cagcttaatg
     2581 ttcgtttttc ttatttatat atttatacca attgattgta tttataactg taaaaatgtg
     2641 tatgttgtgt gcatattttt ttgtgcatgc acatgcatgt aaatagctaa aattatgaac
     2701 attttatttt ttgttcagaa aaaaaaactt tacacacata aaatggctag tatgaatagc
     2761 catattttat ataaattaaa tcctatgaat ttatgaccat attaaaaatt tagatattta
     2821 tggaacataa tatgtttgaa acaataagac aaaattatta ttattattat tatttttact
     2881 gttataatta tgttgtctct tcaatgattc ataaatagtt ggacttgatt tttaaaatgt
     2941 ttataatatg attagcatag ttaaataaaa aaagttgaaa aattaaaaaa aaacatataa
     3001 acacaaatga tgttttttcc ttcaatttcg atatcaattt atagaaacaa aatatatact
     3061 tgtataattt tattttttta tataaatcat tacatatata attatacaat attttttcta
     3121 agagataatt atatattaat atatataaaa aaaggtgttt ttttttttat ttttattttt
     3181 attttatggt aatattttat tttccttatt ttataaatta tattagttta tatgtgatta
     3241 attttatata ttatcaattt atatattttt aaatgcttac ttaattatct tttttttttt
     3301 ttttccttct ttttatattt atttaatttt tgaaaaaatt gatatatata tatatatata
     3361 atatatatat atatacatgt agtagtatta aacaatgtat aatatatata aataatatat
     3421 ttatatattt catttcaatt ttaatttttt tgtttttttt ttctttttgt catatttaaa
     3481 aaaaattata ttcatataag ttatgcattt tttataaaca ttattcaata tatgtataat
     3541 ataatatata tatatatatt aatgtattat tccaatgtgc atgataaaag aaaaaaataa
     3601 tatttataaa aaaaagaaaa ataaaacaaa aaaagaaaaa aaaaaaaaaa atacaaaaat
     3661 aaataatata atttataatt atatattctt gtcacaataa aaatatatat atatatatat
     3721 atatttataa tatgtatatt ttaaactaga aaaggaataa ctaatatttt atttattatc
     3781 attcaagatt tatattttat aataataaat acctaataga aatatatcag gatccatgca
     3841 tggttcgcta aactgcatcg ttgctgtgtc ccagaacatg ggcatcggca agaacgggga
     3901 ctacccctgg ccaccgctca ggaacgaatt tagatatttc cagagaatga ccacaacctc
     3961 ttcagtagaa ggtaaacaga atctggtgat tatgggtaag aagacctggt tctccattcc
     4021 tgagaagaat cgacctttaa agggtagaat taatttagtt ctcagcagag aactcaagga
     4081 acctccacaa ggagctcatt ttctttccag aagtctagat gatgccttaa aacttactga
     4141 acaaccagaa ttagcaaata aagtagacat ggtctggata gttggtggca gttctgttta
     4201 taaggaagcc atgaatcacc caggccatct taaactattt gtgacaagga tcatgcaaga
     4261 ctttgaaagt gacacgtttt ttccagaaat tgatttggag aaatataaac ttctgccaga
     4321 atacccaggt gttctctctg atgtccagga ggagaaaggc attaagtaca aatttgaagt
     4381 atatgagaag aatgattaag cttatttaat aatagattaa aaatattata aaaataaaaa
     4441 cataaacaca gaaattacaa aaaaaataca tatgaatttt tttttgtaat cttccttata
     4501 aatatagaat aatgaatcat ataaaacata tcattattca tttatttaca tttaaaatta
     4561 ttgtttcagt atctttaatt tattatgtat atataaaaat aacttacaat tttattaata
     4621 agcaatatat gtttattaat tcatgttttg taatttatgg gatagcgatt ttttttactg
     4681 tctgtatttt tcttttttaa ttatgtttta attgtatttt atttttatta ttgttctttt
     4741 tatagtatta ttttaaaaca aaatgtattt tctaagaact tataataata ataaatataa
     4801 attttaataa aaattatatt tatcttttac aatatgaaca taaagtacaa cattaatata
     4861 tagcttttaa tatttttatt cctaatcatg taaatcttaa atttttcttt ttaaacatat
     4921 gttaaatatt tatttctcat tatatataag aacatattta ttaaatctag aattcgccct
     4981 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     5041 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     5101 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     5161 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     5221 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     5281 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5341 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     5401 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5461 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5521 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5581 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5641 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5701 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5761 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5821 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5881 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5941 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6001 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6061 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6121 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6181 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6241 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6301 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6361 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6421 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6481 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6541 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6601 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6661 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6721 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6781 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6841 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6901 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6961 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7021 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7081 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7141 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7201 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7261 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7321 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7381 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7441 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7501 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7561 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7621 atgattacgc caagctattt aggtgacact atagaatact caagct
//
