LOCUS       sequence-384305-        4304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control plasmid encoding C-Venus, but without a coiled-coil to drive
            reconstitution..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Rhys GG, Cross JA, Dawson WM, Thompson HF, Shanmugaratnam S, Savery 
            NJ, Dodding MP, Hocker B, Woolfson DN
  TITLE     De novo designed peptides for cellular delivery and subcellular 
            localisation.
  JOURNAL   Nat Chem Biol. 2022 Jul 14. pii: 10.1038/s41589-022-01076-6. doi: 
            10.1038/s41589-022-01076-6.
  PUBMED    35836017
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 Jul 14. pii: 10.1038/s41589-022-01076-6. doi: 
            10.1038/s41589-022-01076-6."
COMMENT     Sequence Label: pBAD-C-Venus
FEATURES             Location/Qualifiers
     source          1..4304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     446..465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     699..716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    731..871
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(797..816)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     957..979
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1135..2013)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2040..2324
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2247..2266
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             2370..2387
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2409..2660
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2597..2618
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2744..2761)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2914..3000
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3092..3119
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3138..3229
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3230..3298,3299..4090)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3448..3467)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4261..4304,1..545)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
       61 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
      121 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      181 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      241 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      301 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      361 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      421 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      481 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      541 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      601 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      661 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      721 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      781 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
      841 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
      901 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
      961 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa
     1021 ttcgcgcgcg aaggcgaagc ggcatgcata atgtgcctgt caaatggacg aagcagggat
     1081 tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga
     1141 caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct
     1201 ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt
     1261 gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat
     1321 acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga
     1381 cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc
     1441 tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg
     1501 gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc
     1561 cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc
     1621 gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga
     1681 cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata
     1741 ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa
     1801 aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg
     1861 aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag
     1921 ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc
     1981 cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga
     2041 agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt
     2101 ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa
     2161 agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga
     2221 ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga
     2281 tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt tttttgggct
     2341 aacaggagga attaaccatg gggggttctc atcatcatca tcatcatggt atggctagcc
     2401 tgcaggggga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     2461 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2521 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca
     2581 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2641 gcatggacga gctgtacaag ggcagcggca gcggcagcgg tctagagggt agtggctgag
     2701 gtaccaagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat
     2761 cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc
     2821 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
     2881 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa
     2941 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     3001 ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc
     3061 ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt
     3121 gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3181 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3241 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3301 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3361 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3421 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     3481 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3541 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3601 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3661 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3721 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3781 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3841 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3901 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3961 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4021 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4081 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4141 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4201 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4261 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaa
//
