LOCUS       sequence-384308-        4390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes C-Venus followed by the antiparallel coiled-coil apCC-Di..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Rhys GG, Cross JA, Dawson WM, Thompson HF, Shanmugaratnam S, Savery 
            NJ, Dodding MP, Hocker B, Woolfson DN
  TITLE     De novo designed peptides for cellular delivery and subcellular 
            localisation.
  JOURNAL   Nat Chem Biol. 2022 Jul 14. pii: 10.1038/s41589-022-01076-6. doi: 
            10.1038/s41589-022-01076-6.
  PUBMED    35836017
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 Jul 14. pii: 10.1038/s41589-022-01076-6. doi: 
            10.1038/s41589-022-01076-6."
COMMENT     Sequence Label: pBAD-C-Venus-apCC-Di
FEATURES             Location/Qualifiers
     source          1..4390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        622..906
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     829..848
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             952..969
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             991..1242
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1179..1200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1412..1429)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1582..1668
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1760..1787
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1806..1897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1898..1966,1967..2758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2116..2135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2929..3517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3418..3437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3671..3688
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3703..3843
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3769..3788)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3929..3951
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(4107..4390,1..595))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta
       61 cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca
      121 attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt
      181 taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga
      241 accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac
      301 gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa
      361 tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat
      421 ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg
      481 gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca
      541 gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt
      601 tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc
      661 actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca
      721 ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc
      781 acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat
      841 ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt
      901 ctccataccc gttttttggg ctaacaggag gaattaacca tggggggttc tcatcatcat
      961 catcatcatg gtatggctag cctgcagggg gacaagcaga agaacggcat caaggccaac
     1021 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     1081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1141 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1201 accgccgccg ggatcactct cggcatggac gagctgtaca agggcagcgg cagcggcagc
     1261 ggtctagagg gtagtggcca gctggaacaa gagttggctg cgctggacca acagattgcc
     1321 gcgctgaaac agcgtcgcgc agctctgaaa tggcagatcc aaggttaggt accaagcttg
     1381 gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa
     1441 gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca
     1501 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga
     1561 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1621 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     1681 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     1741 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
     1801 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1861 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1921 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1981 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     2041 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     2101 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     2161 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     2221 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     2281 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     2341 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2401 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     2461 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2521 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2581 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2641 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2701 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2761 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2821 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2881 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2941 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3001 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3061 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3121 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3181 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3241 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3301 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3361 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3421 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3481 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3541 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3601 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3661 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     3721 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     3781 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     3841 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     3901 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     3961 tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa gcggcatgca
     4021 taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag
     4081 ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc attcactttt
     4141 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
     4201 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
     4261 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
     4321 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc
     4381 tgtgcgacgc
//
