LOCUS       sequence-384310-        4917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning and expression of transcription factor gene under control of
            naringenin inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Bernauw AJ, De Kock V, Bervoets I
  TITLE     In Vivo Screening Method for the Identification and Characterization
            of Prokaryotic, Metabolite-Responsive Transcription Factors.
  JOURNAL   Methods Mol Biol. 2022;2516:113-141. doi: 
            10.1007/978-1-0716-2413-5_8.
  PUBMED    35922625
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2022"; volume: "2516:113-141. doi"; pages: " 
            10.1007/978-1-0716-2413-5_8"
COMMENT     Sequence Label: pITC
FEATURES             Location/Qualifiers
     source          1..4917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    445..478
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(669..1328)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1243..1262
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3716..3938
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             join(3986..4917,1..19)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa gcatcaagac
       61 taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa ctgtccatat
      121 gcacagatga aaacggtgta aaaaagatag atacatcaga gcttttacga gtttttggtg
      181 cattcaaagc tgttcaccat gaacagatcg acaatgtaac agatgaacag catgtaacac
      241 ctaatagaac aggtgaaacc agtaaaacaa agcaactaga acatgaaatt gaacacctga
      301 gacaacttgt tacagctcaa cagtcacaca tagacagcct gaaacaggcg atgctgctta
      361 tcgaagtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcatatg
      421 aatatcctcc ttagttccta ttccgaagtt cctattctct agaaagtata ggaacttcag
      481 agcgcttttg aagctggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
      541 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
      601 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
      661 agtcccgctt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
      721 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc
      781 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc
      841 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
      901 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
      961 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     1021 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     1081 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca
     1141 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     1201 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1261 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     1321 ttctccatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat
     1381 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
     1441 gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc
     1501 tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt
     1561 gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct
     1621 ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcaggatcct
     1681 acaatgcgat atcctaggtc ttacagtatg ccatcatagc ggccgcttac tagaacggta
     1741 ccaggaactt cgaagcagct ccagcctaca cgggagagtg ttcaccgaca aacaacagat
     1801 aaaacaaaag gcccagtctt ccgactgagc cttttgtttt atttgatgtc tggcagttcc
     1861 ccactcgcca gatttacgaa ggctatcagc agatgcctgg cagcgccgca tccatctcct
     1921 gcgcactctc cagaaacacc cgacgcagcc actggatgcc aggatcattg ctgcggtagc
     1981 gatgccactg catcatctgc cgcatctcgc ccagcgacag cggactctcc ttgatcacca
     2041 ccggccattg cggagccagc agctgcgcca gccgggcatg caccgtggcg atgcggtccg
     2101 tcccctgtac cagcgccagc gcagaagcga agctgaagct ggtcacttcc acccggcgcg
     2161 caaagcccag cttcctggcc atccacgcct ccaccgacga cgcattggcc ccaggcggca
     2221 ccatcaccac atggcctgag gccatgtagc gttccagcgt cagctcgcct tgcgccagcg
     2281 cactgtcgcg ccagaccacg cagacatgcc gttcgcggaa gacctcttcg gcaggatgat
     2341 cgggcgtgca gaattcctgc ggcaagacca gcaggtccac ctcggcccga tccagcgagc
     2401 gggtcgggtc ttgcacctgc ggcatcaggg caaagcggat gtgcttgccc tcggcgtgcg
     2461 cgcgcgccag cacccggggg atgaggacgg agagcgtaaa gtccgaaacc gagatgcgaa
     2521 actcgcgcgt ggactcggcc ggcacgaagg ccggcagcgc cgcgatggag ccatcgatac
     2581 gccgcagcac atcatgcacc gcatccttga gcacctcggc gcgcggcgtg ggctccatgc
     2641 gccggcccac ctggatcagc aattcatcat cgaaatactc gcgcagccgc gccagggcat
     2701 tgctcatggc cgactggctc agatggatct tttcggcggc gcggctgatg ctcatctccg
     2761 tgagcagtgc atccagggcg accagaagat tgaggtcgag cttgttgaaa cgcatggcgc
     2821 tggtctccgt tgttgtgctt gttcttgccg accctcggat agacgacgga tggggtggtc
     2881 aatgtattga tgccgtccat atcatgaatc aaaacaatcc atttgatcaa tatcaagctc
     2941 actcttaagc ttcactcatc cgctgcatgg ccccaccaga aagggctggc gcggcaagcc
     3001 ggcggcgcac tcgcactgga tgcgccgctg ttgagcctgg ccatgacaac gcgccgatag
     3061 cggccacacc ccgccaggca gggtaggaga caaggagaca gccatggata ctgagtgagt
     3121 cgacatcgtc tcgagtcgta atcggctaac aactgtcaac cttgcgttag cgtacagatg
     3181 ctcttgttca gaacgctcgg tcttgcacac cgggcgtttt ttctttgtga gtccacgaca
     3241 gtaagacggg taagcctgtt gatgataccg ctgccttact gggtgcatta gccagtctga
     3301 atgacctgtc acgggataat ccgaagtggt cagactggaa aatcagaggg caggaactgc
     3361 tgaacagcaa aaagtcagat agcaccacat agcagacccg ccataaaacg ccctgagaag
     3421 cccgtgacgg gcttttcttg tattatgggt agtttccttg catgaatcca taaaaggcgc
     3481 ctgtagtgcc atttaccccc attcactgcc agagccgtga gcgcagcgaa ctgaatgtca
     3541 cgaaaaagac agcgactcag gtgcctgatg gtcggagaca aaaggaatat tcagcgattt
     3601 gcccgagctt gcgagggtgc tacttaagcc tttagggttt taaggtctgt tttgtagagg
     3661 agcaaacagc gtttgcgaca tccttttgta atactgcgga actgactaaa gtagtgagtt
     3721 atacacaggg ctgggatcta ttctttttat ctttttttat tctttcttta ttctataaat
     3781 tataaccact tgaatataaa caaaaaaaac acacaaaggt ctagcggaat ttacagaggg
     3841 tctagcagaa tttacaagtt ttccagcaaa ggtctagcag aatttacaga tacccacaac
     3901 tcaaaggaaa aggactagta attatcattg actagcccat ctcaattggt atagtgatta
     3961 aaatcaccta gaccaattga gatgtatgtc tgaattagtt gttttcaaag caaatgaact
     4021 agcgattagt cgctatgact taacggagca tgaaaccaag ctaattttat gctgtgtggc
     4081 actactcaac cccacgattg aaaaccctac aaggaaagaa cggacggtat cgttcactta
     4141 taaccaatac gctcagatga tgaacatcag tagggaaaat gcttatggtg tattagctaa
     4201 agcaaccaga gagctgatga cgagaactgt ggaaatcagg aatcctttgg ttaaaggctt
     4261 tgagattttc cagtggacaa actatgccaa gttctcaagc gaaaaattag aattagtttt
     4321 tagtgaagag atattgcctt atcttttcca gttaaaaaaa ttcataaaat ataatctgga
     4381 acatgttaag tcttttgaaa acaaatactc tatgaggatt tatgagtggt tattaaaaga
     4441 actaacacaa aagaaaactc acaaggcaaa tatagagatt agccttgatg aatttaagtt
     4501 catgttaatg cttgaaaata actaccatga gtttaaaagg cttaaccaat gggttttgaa
     4561 accaataagt aaagatttaa acacttacag caatatgaaa ttggtggttg ataagcgagg
     4621 ccgcccgact gatacgttga ttttccaagt tgaactagat agacaaatgg atctcgtaac
     4681 cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa ataccaacaa ccattacatc
     4741 agattcctac ctacataacg gactaagaaa aacactacac gatgctttaa ctgcaaaaat
     4801 tcagctcacc agttttgagg caaaattttt gagtgacatg caaagtaagt atgatctcaa
     4861 tggttcgttc tcatggctca cgcaaaaaca acgaaccaca ctagagaaca tactggc
//
