LOCUS       sequence-384346-        1388 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Human N-Terminal 3X Flag CASP9 Catalytic Mutant in 
            Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Han J, Goldstein LA, Hou W, Watkins SC, Rabinowich H
  TITLE     Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal 
            transport.
  JOURNAL   Autophagy. 2021 Jun;17(6):1393-1409. doi: 
            10.1080/15548627.2020.1761742. Epub 2020 May 25.
  PUBMED    32397873
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2020.1761742"; journalName: "Autophagy"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "1393-1409"
COMMENT     Sequence Label: N-terminal 3X Flag Human CASP9 C287A Mutant
FEATURES             Location/Qualifiers
     source          1..1388
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..96
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(826..837)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 aagcttggta ccgagctcgg atccgccatg gactacaaag accatgacgg tgattataaa
       61 gatcatgaca tcgattacaa ggatgacgat gacaagatgg acgaagcgga tcggcggctc
      121 ctgcggcggt gccggctgcg gctggtggaa gagctgcagg tggaccagct ctgggacgcc
      181 ctgctgagcc gcgagctgtt caggccccat atgatcgagg acatccagcg ggcaggctct
      241 ggatctcggc gggatcaggc caggcagctg atcatagatc tggagactcg agggagtcag
      301 gctcttcctt tgttcatctc ctgcttagag gacacaggcc aggacatgct ggcttcgttt
      361 ctgcgaacta acaggcaagc agcaaagttg tcgaagccaa ccctagaaaa ccttacccca
      421 gtggtgctca gaccagagat tcgcaaacca gaggttctca gaccggaaac acccagacca
      481 gtggacattg gttctggagg atttggtgat gtcggtgctc ttgagagttt gaggggaaat
      541 gcagatttgg cttacatcct gagcatggag ccctgtggcc actgcctcat tatcaacaat
      601 gtgaacttct gccgtgagtc cgggctccgc acccgcactg gctccaacat cgactgtgag
      661 aagttgcggc gtcgcttctc ctcgctgcat ttcatggtgg aggtgaaggg cgacctgact
      721 gccaagaaaa tggtgctggc tttgctggag ctggcgcagc aggaccacgg tgctctggac
      781 tgctgcgtgg tggtcattct ctctcacggc tgtcaggcca gccacctgca gttcccaggg
      841 gctgtctacg gcacagatgg atgccctgtg tcggtcgaga agattgtgaa catcttcaat
      901 gggaccagct gccccagcct gggagggaag cccaagctct ttttcatcca ggccgcgggt
      961 ggggagcaga aagaccatgg gtttgaggtg gcctccactt cccctgaaga cgagtcccct
     1021 ggcagtaacc ccgagccaga tgccaccccg ttccaggaag gtttgaggac cttcgaccag
     1081 ctggacgcca tatctagttt gcccacaccc agtgacatct ttgtgtccta ctctactttc
     1141 ccaggttttg tttcctggag ggaccccaag agtggctcct ggtacgttga gaccctggac
     1201 gacatctttg agcagtgggc tcactctgaa gacctgcagt ccctcctgct tagggtcgct
     1261 aatgctgttt cggtgaaagg gatttataaa cagatgcctg gttgctttaa tttcctccgg
     1321 aaaaaacttt tctttaaaac atcataaggc cagggcccct caccctgcct tatcttgcag
     1381 tcgagtct
//
