LOCUS       sequence-38435-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Weinberg DE, Nakanishi K, Patel DJ, Bartel DP
  TITLE     The inside-out mechanism of dicers from budding yeasts.
  JOURNAL   Cell. 2011 Jul 22;146(2):262-76.
  PUBMED    21784247
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-22"; volume: "146(2)"; pages: "262-76"
COMMENT     Sequence Label: pRSF-KpDcr1deltaC(N184A)
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             103..396
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             515..526
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atattttgtt taactttaat aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc cacatggcaa gcatgtcgga ctcagaagtc
      121 aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta
      181 aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga
      241 aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg
      301 tacgacggta ttagaatcca agctgatcag acccctgaag atttggacat ggaggataac
      361 gatattattg aggctcacag agaacagatt ggtggatcca acgagttaaa agttagagaa
      421 ttttatagac tccacaatgc ttgtgtgaaa ttgaaagagt ctattaaatt gatttatgaa
      481 aacccattgg taactgacca aaatgttttg aatttgggaa ctgcagaaaa tactattgat
      541 tatacaattt taaacacacc tacactaaat gttgctaaaa ctttactggg caatagatat
      601 tcattagatc taatagatct ttttcaatct catgatttca aagattcaaa cactgatgtg
      661 gatatgttta ttaaatatcc tgtggtttat gatgaaaatt tggaaaattt agcatttatg
      721 cataaaagtt ttccaaatat gaatgcacat ctaaatgatg ctcaaaagac tcaattatct
      781 aatgaaaggt tagaattttt gggtgatagt tggctaggtg ctttagtttc atatattgta
      841 tacaccagat ttccttctgc aaatga
//
