LOCUS       sequence-384351-        5096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry reporter containing 13 copies of the p53-binding consensus 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, 
            Oguro H, Kanaujiya J, Zou J, Sebesta D, Pratt G, Cross E, Blockwick 
            J, Buxton P, Kinner-Bibeau L, Medura C, Tompkins C, Hughes S, 
            Santiana M, Faghri F, Nalls MA, Vitale D, Ballard S, Qi YA, Ramos 
            DM, Anderson KM, Stadler J, Narayan P, Papademetriou J, Reilly L, 
            Nelson MP, Aggarwal S, Rosen LU, Kirwan P, Pisupati V, Coon SL, 
            Scholz SW, Priebe T, Ottl M, Dong J, Meijer M, Janssen LJM, Lourenco
            VS, van der Kant R, Crusius D, Paquet D, Raulin AC, Bu G, Held A, 
            Wainger BJ, Gabriele RMC, Casey JM, Wray S, Abu-Bonsrah D, Parish 
            CL, Beccari MS, Cleveland DW, Li E, Rose IVL, Kampmann M, Calatayud 
            Aristoy C, Verstreken P, Heinrich L, Chen MY, Schule B, Dou D, 
            Holzbaur ELF, Zanellati MC, Basundra R, Deshmukh M, Cohen S, Khanna 
            R, Raman M, Nevin ZS, Matia M, Van Lent J, Timmerman V, Conklin BR, 
            Johnson Chase K, Zhang K, Funes S, Bosco DA, Erlebach L, Welzer M, 
            Kronenberg-Versteeg D, Lyu G, Arenas E, Coccia E, Sarrafha L, 
            Ahfeldt T, Marioni JC, Skarnes WC, Cookson MR, Ward ME, Merkle FT
  TITLE     A reference human induced pluripotent stem cell line for large-scale
            collaborative studies.
  JOURNAL   Cell Stem Cell. 2022 Dec 1;29(12):1685-1702.e22. doi: 
            10.1016/j.stem.2022.11.004.
  PUBMED    36459969
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2022.11"; journalName: "Cell Stem Cell"; date: 
            "2022-12-1- 1"; volume: "29"; issue: "12"; pages: "1685-1702.e22"
COMMENT     Sequence Label: PG13-mCherry
FEATURES             Location/Qualifiers
     source          1..5096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(421..437)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             491..1201
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(636..654)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     904..923
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          1332..1397
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1527..1547
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1972..2106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2009..2028)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2063..2082
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2103..2119)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2156..2174)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2195..2211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2195..2211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2208..2230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2219..2235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2243..2249,2250..2267,2268..2273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2288..2309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2426..2443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2597..3185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2677..2696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3356..4147,4148..4216))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3979..3998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4217..4321)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4347..4802)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4484..4505)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4696..4715
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4929..4951
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4943..4960
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4944..4960
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4967..4986
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4967..4985
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     5010..5026
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5011..5027
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccaggcaagt ccaggcaggc caggcaagtc caggcaggcc aggcaagtcc aggcaggcca
       61 ggcaagtcca ggcaggccag gcaagtccag gcaggccagg caagtccagg caggccaggc
      121 aagtccaggc agggccaggc aagtccaggc aggccaggca agtccaggca ggccaggcaa
      181 gtccaggcag gatcgaattc gatctgtttt ttttagtatt aagcagaggc cgggggcccc
      241 tggcctccgc ttactctgga gaaaaagaag agaggcattc cagaggcaac ttgtcaaaac
      301 aggactggcg ccttggaggc gctgtggggc cacccaaatt gatataatta agccccaacc
      361 gcctcttccc gcctcatttc agcctcacca ccctcgacaa gcttatcgat accgtcgggg
      421 gatccactag ttctagagcg gccgccaccg cggtggagct cgagcttggc attccggtac
      481 tgttggtaaa atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
      541 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
      601 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
      661 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
      721 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
      781 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
      841 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
      901 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
      961 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     1021 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     1081 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1141 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     1201 gaatgtaact gtattcagcg atgacgaaat tcttagctat tgtaatactc tagaggatct
     1261 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
     1321 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
     1381 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
     1441 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
     1501 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
     1561 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
     1621 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
     1681 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt
     1741 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta
     1801 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga
     1861 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     1921 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     1981 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2041 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     2101 tggatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
     2161 agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     2221 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2281 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2341 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2401 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2461 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2761 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3001 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4321 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     4381 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     4441 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     4501 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     4561 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     4621 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     4681 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     4741 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     4801 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     4861 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     4921 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     4981 atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatgc
     5041 caggcaagtc caggcaggcc aggcaagtcc aggcaggcca ggcaagtcca ggcagg
//
