LOCUS       sequence-38436-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Weinberg DE, Nakanishi K, Patel DJ, Bartel DP
  TITLE     The inside-out mechanism of dicers from budding yeasts.
  JOURNAL   Cell. 2011 Jul 22;146(2):262-76.
  PUBMED    21784247
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-22"; volume: "146(2)"; pages: "262-76"
COMMENT     Sequence Label: pRSF-KpDcr1deltaC(K217A)
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             104..397
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             516..527
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ataattttgt ttaactttaa taaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggtgc cgcgcggcag ccacatggca agcatgtcgg actcagaagt
      121 caatcaagaa gctaagccag aggtcaagcc agaagtcaag cctgagactc acatcaattt
      181 aaaggtgtcc gatggatctt cagagatctt cttcaagatc aaaaagacca ctcctttaag
      241 aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa atggactcct taagattctt
      301 gtacgacggt attagaatcc aagctgatca gacccctgaa gatttggaca tggaggataa
      361 cgatattatt gaggctcaca gagaacagat tggtggatcc aacgagttaa aagttagaga
      421 attttataga ctccacaatg cttgtgtgaa attgaaagag tctattaaat tgatttatga
      481 aaacccattg gtaactgacc aaaatgtttt gaatttggga actgcagaaa atactattga
      541 ttatacaatt ttaaacacac ctacactaaa tgttgctaaa actttactgg gcaatagata
      601 ttcattagat ctaatagatc tttttcaatc tcatgatttc aaagattcaa acactgatgt
      661 ggatatgttt attaaatatc ctgtggttta tgatgaaaat ttggaaaatt tagcatttat
      721 gcataaaagt tttccaaata tgaatgcaca tctaaatgat gctcaaaaga ctcaattatc
      781 taatgaaagg ttagaatttt tgggtgatag ttggctaggt gctttagttt catatattgt
      841 atacaccaga tttccttctg caaatgaagg tatgctttct ca
//
